scholarly journals Detection of toxigenic Vibrio cholerae by PCR

2011 ◽  
Vol 5 (1) ◽  
pp. 18-21
Author(s):  
Majeed Arsheed Sabbah ◽  
Bilal Kamil Sulaiman ◽  
Kifah, A. Jasim ◽  
Mohammod M. Farhan
Keyword(s):  
Cholera Toxin B Subunit ◽  
Specific Primers ◽  
Toxin B ◽  
E Coli ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Conventional Culture ◽  
Major Virulence Factor ◽  
Ctxb Gene ◽  
Direct Use

holera toxin (CT) is a major virulence factor of V. cholerae causing water diarrhea. The detection of CT-producing V. cholerae using conventional culture-, biochemical- and immunological-based assays is time-consuming, laborious, and requiring more than three days perform. In this work a specific primers for ctxB gene were used for detection of V. cholera in water samples. Few colonies of V. cholera were suspended in water and used as a template in PCR reaction for the detection of ctxB gene. The 391-bp sequence of a gene that codes for the cholera toxin B subunit was amplified by PCR. Direct use of V. cholerae pure culture for PCR replaces the need for DNA extraction or boiling. Increase the concentration of MgCl2 enhances the efficiency of amplification. The specificity of the assay was determined to be specific for V. cholerae but not for, vibrio related bacteria, E. coli, Non-Agglutinable (NAG) V. cholerae, and Aeromonas sp.

scholarly journals The B subunits of cholera and Escherichia coli heat-labile toxins enhance the immune responses in mice orally immunised with a recombinant live P-fimbrial vaccine for avian pathogenic E. coli

2014 ◽  
Vol 62 (3) ◽  
pp. 293-303
Author(s):  
In-Gyeong Oh ◽  
Chetan Jawale ◽  
John Lee
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Cholera Toxin B Subunit ◽  
Adjuvant Effect ◽  
Toxin B ◽  
E Coli ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Heat Labile ◽  
Enterotoxin B

This study aimed to investigate the adjuvant effect of recombinant attenuatedSalmonellaexpressing cholera toxin B subunit (CTB) andEscherichia coliheat-labile enterotoxin B subunit (LTB) for the P-fimbriae subunit-based vaccine of avian pathogenicE. coli(APEC) in a murine model. The PapA-specific sIgA and IgG responses were significantly enhanced after immunisation with theSalmonella-PapA vaccine in the presence of CTB or LTB. The group immunised with theSalmonella-LTB strain promoted Th1-type immunity, whereas that immunised with theSalmonella-CTB strain produced Th2-type immunity. We concluded that bothSalmonella-CTB and -LTB strains can enhance the immune response to PapA, and that the LTB strain may be a more effective adjuvant for APEC vaccination, which requires higher Th1-type immunity for protection. Thus, our findings provide evidence that immunisation with an adjuvant, LTB, is one of the strategies of developing effective vaccines against P-fimbriated APEC.

Immunogenicity of a Cholera Toxin B Subunit Porphyromonas gingivalis Fimbrial Antigen Fusion Protein Expressed in E. coli

2008 ◽  
Vol 41 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Tae-Geum Kim ◽  
Nguyen-Xuan Huy ◽  
Mi-Young Kim ◽  
Dong-Keun Jeong ◽  
Yong-Suk Jang ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cholera Toxin ◽  
Porphyromonas Gingivalis ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
E Coli ◽  
Cholera Toxin B ◽  
B Subunit

Gene fusion of cholera toxin B subunit with HBV PreS epitope and overexpression in E. coli

Vaccine ◽  
1992 ◽  
Vol 10 (4) ◽  
pp. 282
Author(s):  
Shi Chengua ◽  
Chao Chen ◽  
Zhang Jingshen ◽  
Ma Quingjun
Keyword(s):  
Cholera Toxin ◽  
Gene Fusion ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
E Coli ◽  
Cholera Toxin B ◽  
B Subunit

scholarly journals Spray-Dried Formulation of Epicertin, a Recombinant Cholera Toxin B Subunit Variant That Induces Mucosal Healing

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 576
Author(s):  
Micaela A. Reeves ◽  
Joshua M. Royal ◽  
David A. Morris ◽  
Jessica M. Jurkiewicz ◽  
Nobuyuki Matoba ◽  
...  
Keyword(s):  
Gastric Acid ◽  
Cholera Toxin ◽  
Oral Formulation ◽  
Size Exclusion ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Enteric Coated ◽  
Spray Dried

Epicertin (EPT) is a recombinant variant of the cholera toxin B subunit, modified with a C-terminal KDEL endoplasmic reticulum retention motif. EPT has therapeutic potential for ulcerative colitis treatment. Previously, orally administered EPT demonstrated colon epithelial repair activity in dextran sodium sulfate (DSS)-induced acute and chronic colitis in mice. However, the oral dosing requires cumbersome pretreatment with sodium bicarbonate to conserve the acid-labile drug substance while transit through the stomach, hampering its facile application in chronic disease treatment. Here, we developed a solid oral formulation of EPT that circumvents degradation in gastric acid. EPT was spray-dried and packed into enteric-coated capsules to allow for pH-dependent release in the colon. A GM1-capture KDEL-detection ELISA and size-exclusion HPLC indicated that EPT powder maintains activity and structural stability for up to 9 months. Capsule disintegration tests showed that EPT remained encapsulated at pH 1 but was released over 180 min at pH 6.8, the approximate pH of the proximal colon. An acute DSS colitis study confirmed the therapeutic efficacy of encapsulated EPT in C57BL/6 mice upon oral administration without gastric acid neutralization pretreatment compared to vehicle-treated mice (p < 0.05). These results provide a foundation for an enteric-coated oral formulation of spray-dried EPT.

Sign in / Sign up

Export Citation Format

Share Document