Humoral and T cell-mediated immune responses to bivalent killed bovine viral diarrhea virus vaccine in beef cattle

2008 ◽  
Vol 122 (1-2) ◽  
pp. 8-15 ◽  
Author(s):  
Ratree Platt ◽  
Christopher Coutu ◽  
Todd Meinert ◽  
James A. Roth
Keyword(s):  
T Cell ◽  
Immune Responses ◽  
Beef Cattle ◽  
Virus Vaccine ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Quillaja brasiliensis saponins induce robust humoral and cellular responses in a bovine viral diarrhea virus vaccine in mice

2016 ◽  
Vol 45 ◽  
pp. 1-8 ◽  
Author(s):  
Samuel Paulo Cibulski ◽  
Fernando Silveira ◽  
Gustavo Mourglia-Ettlin ◽  
Thais Fumaco Teixeira ◽  
Helton Fernandes dos Santos ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Bovine Viral Diarrhea Virus ◽  
Cellular Responses ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

scholarly journals Priming with DNA encoding E2 and boosting with E2 protein formulated with CpG oligodeoxynucleotides induces strong immune responses and protection from Bovine viral diarrhea virus in cattle

2006 ◽  
Vol 87 (10) ◽  
pp. 2971-2982 ◽  
Author(s):  
Rong Liang ◽  
Jan V. van den Hurk ◽  
Lorne A. Babiuk ◽  
Sylvia van Drunen Littel-van den Hurk
Keyword(s):  
Immune Responses ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
E2 Protein ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Boost Vaccination ◽  
Viral Excretion ◽  
Protein Boost ◽  
Viral Diarrhea Virus

The objective of this study was to develop an optimal vaccination strategy for Bovine viral diarrhea virus (BVDV). The E2 protein of BVDV plays a major protective role against BVDV infection. In order to be able to compare DNA, protein and DNA prime–protein boost regimens, a plasmid was constructed encoding a secreted form of the NADL strain E2 protein (pMASIA-tPAsΔE2). Furthermore, a pure secreted recombinant ΔE2 (rΔE2) protein was produced. The rΔE2 protein was formulated with a combination of Emulsigen and CpG oligodeoxynucleotide. Groups of calves were immunized with pMASIA-tPAsΔE2 or with rΔE2, or first with pMASIA-tPAsΔE2 and then with rΔE2. To evaluate the protection against BVDV, calves were challenged with BVDV strain NY-1 after the last immunization. Although all immunized calves developed humoral and cellular immune responses, the antibody responses in the DNA prime–protein boost group were stronger than those elicited by either the DNA vaccine or the protein vaccine. In particular, E2-specific antibody titres were enhanced significantly after boosting the ΔE2 DNA-primed calves with rΔE2 protein. Moreover, protection against BVDV challenge was obtained in the calves treated with the DNA prime–protein boost vaccination regimen, as shown by a significant reduction in weight loss, viral excretion and lymphopenia, compared with the unvaccinated calves and the animals immunized with the DNA or protein only. These results demonstrate the advantage of a DNA prime–protein boost vaccination approach in an outbred species.

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