Genome editing of a hybridoma cell line via the CRISPR/Cas9 system: A new approach for constitutive high-level expression of heterologous proteins in eukaryotic system

2021 ◽  
pp. 110286
Author(s):  
Nicoletta Schibeci Natoli Scialli ◽  
Barbara Colitti ◽  
Luigi Bertolotti ◽  
Giulia Pezzoni ◽  
Eugenio Martignani ◽  
...  
Keyword(s):  
Cell Line ◽  
Genome Editing ◽  
Heterologous Proteins ◽  
High Level Expression ◽  
New Approach ◽  
System A ◽  
High Level

scholarly journals Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines

Blood ◽  
1986 ◽  
Vol 68 (5) ◽  
pp. 1101-1107 ◽  
Author(s):  
AJ Furley ◽  
BR Reeves ◽  
S Mizutani ◽  
LJ Altass ◽  
SM Watt ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Myeloid Cell ◽  
Cell Receptor ◽  
High Level Expression ◽  
Igh Genes ◽  
T Cell Receptor Beta ◽  
Molecular Phenotypes ◽  
High Level ◽  
Receptor Beta

The cell line KG1 derived from a patient with erythroleukemia in myeloblastic relapse has the composite phenotype and functional repertoire of myeloblasts. In marked contrast, its subline KG1a has lost myeloid features, acquired new karyotypic markers, and has three characteristics associated with immature T cells: low-level expression of the T cell receptor beta mRNA (but not alpha) transcribed from a germline gene; high-level expression of T3 delta mRNA and intracellular, but not cell surface, T3 protein; and expression of the CD7/gp40 T cell-associated membrane antigen. Both KG1 and KG1a transcribe unrearranged IgH genes. These data suggest that either the KG1 cell line was derived from a common myeloid-lymphoid progenitor or that the KG1a subline phenotype is aberrant.

scholarly journals LCR/MEL: A versatile system for high-level expression of heterologous proteins in erythroid cells

1992 ◽  
Vol 20 (5) ◽  
pp. 997-1003 ◽  
Author(s):  
M. Needham ◽  
C. Gooding ◽  
K. Hudson ◽  
M. Antoniou ◽  
F. Grosveld ◽  
...  
Keyword(s):  
Heterologous Proteins ◽  
Erythroid Cells ◽  
High Level Expression ◽  
High Level ◽  
In Erythroid Cells

High-Fidelity Translation of Recombinant Human Hemoglobin in Escherichia coli

1998 ◽  
Vol 64 (5) ◽  
pp. 1589-1593 ◽  
Author(s):  
Michael J. Weickert ◽  
Izydor Apostol
Keyword(s):  
Escherichia Coli ◽  
Error Rates ◽  
High Fidelity ◽  
Human Hemoglobin ◽  
Heterologous Proteins ◽  
Expression Systems ◽  
High Level Expression ◽  
E Coli ◽  
Translation Error ◽  
High Level

ABSTRACT Coexpression of di-α-globin and β-globin in Escherichia coli in the presence of exogenous heme yielded high levels of soluble, functional recombinant human hemoglobin (rHb1.1). High-level expression of rHb1.1 provides a good model for measuring mistranslation in heterologous proteins. rHb1.1 does not contain isoleucine; therefore, any isoleucine present could be attributed to mistranslation, most likely mistranslation of one or more of the 200 codons that differ from an isoleucine codon by 1 bp. Sensitive amino acid analysis of highly purified rHb1.1 typically revealed ≤0.2 mol of isoleucine per mol of hemoglobin. This corresponds to a translation error rate of ≤0.001, which is not different from typical translation error rates found for E. coli proteins. Two different expression systems that resulted in accumulation of globin proteins to levels equivalent to ∼20% of the level of E. colisoluble proteins also resulted in equivalent translational fidelity.

High-level expression and purification of the human bradykinin B2 receptor in a tetracycline-inducible stable HEK293S cell line

2007 ◽  
Vol 55 (2) ◽  
pp. 300-311 ◽  
Author(s):  
Paméla Camponova ◽  
Stéphanie Baud ◽  
Hélène Mattras ◽  
Isabelle Duroux-Richard ◽  
Jean-Claude Bonnafous ◽  
...  
Keyword(s):  
Cell Line ◽  
Expression And Purification ◽  
High Level Expression ◽  
Bradykinin B2 Receptor ◽  
High Level

New Approach to Achieve High-Level Secretory Expression of Heterologous Proteins by Using Tat Signal Peptide

2009 ◽  
Vol 16 (6) ◽  
pp. 706-710 ◽  
Author(s):  
Yu-Dong Li ◽  
Zhan Zhou ◽  
Long-Xian Lv ◽  
Xiao-Ping Hou ◽  
Yong-Quan Li
Keyword(s):  
Signal Peptide ◽  
Heterologous Proteins ◽  
Secretory Expression ◽  
New Approach ◽  
High Level

scholarly journals Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines

Blood ◽  
1986 ◽  
Vol 68 (5) ◽  
pp. 1101-1107 ◽  
Author(s):  
AJ Furley ◽  
BR Reeves ◽  
S Mizutani ◽  
LJ Altass ◽  
SM Watt ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Myeloid Cell ◽  
Cell Receptor ◽  
High Level Expression ◽  
Igh Genes ◽  
T Cell Receptor Beta ◽  
Molecular Phenotypes ◽  
High Level ◽  
Receptor Beta

Abstract The cell line KG1 derived from a patient with erythroleukemia in myeloblastic relapse has the composite phenotype and functional repertoire of myeloblasts. In marked contrast, its subline KG1a has lost myeloid features, acquired new karyotypic markers, and has three characteristics associated with immature T cells: low-level expression of the T cell receptor beta mRNA (but not alpha) transcribed from a germline gene; high-level expression of T3 delta mRNA and intracellular, but not cell surface, T3 protein; and expression of the CD7/gp40 T cell-associated membrane antigen. Both KG1 and KG1a transcribe unrearranged IgH genes. These data suggest that either the KG1 cell line was derived from a common myeloid-lymphoid progenitor or that the KG1a subline phenotype is aberrant.

High-level expression of cytokine-induced neutrophil chemoattractant (CINC) by a metastatic rat cell line: Purification and production of blocking antibodies

1993 ◽  
Vol 156 (2) ◽  
pp. 421-427 ◽  
Author(s):  
Arthur J. Wittwer ◽  
Linda S. Carr ◽  
John Zagorski ◽  
Gregory J. Dolecki ◽  
Barbara A. Crippes ◽  
...  
Keyword(s):  
Cell Line ◽  
High Level Expression ◽  
High Level ◽  
Blocking Antibodies
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