New Approach to Achieve High-Level Secretory Expression of Heterologous Proteins by Using Tat Signal Peptide

2009 ◽  
Vol 16 (6) ◽  
pp. 706-710 ◽  
Author(s):  
Yu-Dong Li ◽  
Zhan Zhou ◽  
Long-Xian Lv ◽  
Xiao-Ping Hou ◽  
Yong-Quan Li
Keyword(s):  
Signal Peptide ◽  
Heterologous Proteins ◽  
Secretory Expression ◽  
New Approach ◽  
High Level

High-Level Secretory Expression and Purification of Recombinant Human Interleukin 1 Beta in Pichia pastoris

2016 ◽  
Vol 23 (8) ◽  
pp. 763-769 ◽  
Author(s):  
Pengfei Li ◽  
Ganggang Yang ◽  
Xiaofang Geng ◽  
Jinbao Shi ◽  
Bin Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Expression And Purification ◽  
Secretory Expression ◽  
High Level

scholarly journals Molecular Recognition: Perspective and a New Approach

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2757
Author(s):  
W. Rudolf Seitz ◽  
Casey J. Grenier ◽  
John R. Csoros ◽  
Rongfang Yang ◽  
Tianyu Ren
Keyword(s):  
Molecular Recognition ◽  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Binding Kinetics ◽  
Molecularly Imprinted ◽  
New Approach ◽  
Imprinted Polymers ◽  
High Affinity ◽  
Water Blocking ◽  
High Level

This perspective presents an overview of approaches to the preparation of molecular recognition agents for chemical sensing. These approaches include chemical synthesis, using catalysts from biological systems, partitioning, aptamers, antibodies and molecularly imprinted polymers. The latter three approaches are general in that they can be applied with a large number of analytes, both proteins and smaller molecules like drugs and hormones. Aptamers and antibodies bind analytes rapidly while molecularly imprinted polymers bind much more slowly. Most molecularly imprinted polymers, formed by polymerizing in the presence of a template, contain a high level of covalent crosslinker that causes the polymer to form a separate phase. This results in a material that is rigid with low affinity for analyte and slow binding kinetics. Our approach to templating is to use predominantly or exclusively noncovalent crosslinks. This results in soluble templated polymers that bind analyte rapidly with high affinity. The biggest challenge of this approach is that the chains are tangled when the templated polymer is dissolved in water, blocking access to binding sites.

4D Einstein equations in a general gauge Kaluza–Klein space

2015 ◽  
Vol 12 (03) ◽  
pp. 1550036
Author(s):  
Aurel Bejancu ◽  
Constantin Călin
Keyword(s):  
Einstein Equations ◽  
Tensor Fields ◽  
New Approach ◽  
Higher Dimensional ◽  
High Level ◽  
General Gauge ◽  
Kaluza Klein

Using the new approach on higher-dimensional Kaluza–Klein theories developed by the first author, we obtain the 4D Einstein equations on a (4 + n)D relativistic gauge Kaluza–Klein space. Adapted frame and coframe fields, adapted tensor fields, and the Riemannian adapted connection, have a fundamental role in the study. The high level of generality of the study, enables us to recover several results from earlier papers on this matter.

ACRIPPER: A New Associative Classification Based on RIPPER Algorithm

2021 ◽  
Vol 20 (01) ◽  
pp. 2150013
Author(s):  
Mohammed Abu-Arqoub ◽  
Wael Hadi ◽  
Abdelraouf Ishtaiwi
Keyword(s):  
Decision Making ◽  
Class Imbalance ◽  
The Other ◽  
Associative Classification ◽  
Rule Based ◽  
New Approach ◽  
New Novel ◽  
Accuracy Measure ◽  
High Level ◽  
Substantial Interest

Associative Classification (AC) classifiers are of substantial interest due to their ability to be utilised for mining vast sets of rules. However, researchers over the decades have shown that a large number of these mined rules are trivial, irrelevant, redundant, and sometimes harmful, as they can cause decision-making bias. Accordingly, in our paper, we address these challenges and propose a new novel AC approach based on the RIPPER algorithm, which we refer to as ACRIPPER. Our new approach combines the strength of the RIPPER algorithm with the classical AC method, in order to achieve: (1) a reduction in the number of rules being mined, especially those rules that are largely insignificant; (2) a high level of integration among the confidence and support of the rules on one hand and the class imbalance level in the prediction phase on the other hand. Our experimental results, using 20 different well-known datasets, reveal that the proposed ACRIPPER significantly outperforms the well-known rule-based algorithms RIPPER and J48. Moreover, ACRIPPER significantly outperforms the current AC-based algorithms CBA, CMAR, ECBA, FACA, and ACPRISM. Finally, ACRIPPER is found to achieve the best average and ranking on the accuracy measure.

scholarly journals A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Cheng Cheng ◽  
Shanshan Wu ◽  
Lupeng Cui ◽  
Yulu Wu ◽  
Tianyue Jiang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion System ◽  
Heterologous Proteins ◽  
Secretory Expression

High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase

2012 ◽  
Vol 47 (1) ◽  
pp. 151-155 ◽  
Author(s):  
Sufang Zhang ◽  
Fan Yang ◽  
Qian Wang ◽  
Yanyan Hua ◽  
Zongbao K. Zhao
Keyword(s):  
Kluyveromyces Marxianus ◽  
Secretory Expression ◽  
High Level

scholarly journals HISTORY EDUCATION IN CURRICULUM 2013: A NEW APPROACH TO TEACHING HISTORY

2013 ◽  
Vol 14 (1) ◽  
pp. 163 ◽  
Author(s):  
Said Hamid Hasan
Keyword(s):  
Lesson Plan ◽  
Student Learning Outcomes ◽  
Historical Events ◽  
Paper Test ◽  
New Approach ◽  
Teaching History ◽  
Assessment Of Student Learning ◽  
Pencil And Paper ◽  
Amount Of Knowledge ◽  
High Level

There has been so many findings and thoughts indicating that teaching history is dominated by what teachers tell of what happened in the past as it is written in the textbooks or reference books. Students listen, read, and memorize the narration and the more details the student could memorize the higher mark she/he will get. The plan for teaching history, syllabus or lesson plan, shows a high level of consistency amongst the learning objectives, teaching processes, and assessment of student learning outcomes. Memorize of historical facts and the reproduction of historical narratives as indication of student’s understanding of historical events, the transmission of information from teacher to student to realize the objectives, and the use of pencil and paper test to assess student level of achievement are the common practices in school from primary to secondary education. It would be no surprise if teaching history at the higher level of education would follow the same path. Public still consider a good scholar or historian in this case, is measured by the amount of knowledge she/he can memorize and she/he should be able to answer some many questions of the facts of historical events. In fact, there is no question of how many a student has in her/his memory apart from what is questioned.

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