Evaluation of the suitability of six host genes as internal control in real-time RT-PCR assays in chicken embryo cell cultures infected with infectious bursal disease virus

2005 ◽  
Vol 110 (3-4) ◽  
pp. 155-165 ◽  
Author(s):  
Y LI ◽  
D BANG ◽  
K HANDBERG ◽  
P JORGENSEN ◽  
M ZHANG
Keyword(s):  
Cell Cultures ◽  
Internal Control ◽  
Infectious Bursal Disease Virus ◽  
Embryo Cell ◽  
Infectious Bursal Disease ◽  
Rt Pcr ◽  
Bursal Disease ◽  
Pcr Assays ◽  
Host Genes ◽  
Chicken Embryo Cell

A Practical Tissue Sampling Method Using Ordinary Paper for Molecular Detection of Infectious Bursal Disease Virus RNA by RT-PCR

2006 ◽  
Vol 50 (4) ◽  
pp. 556-560 ◽  
Author(s):  
Min Thein Maw ◽  
Tsuyoshi Yamaguchi ◽  
Christopher J. Kasanga ◽  
Kaori Terasaki ◽  
Hideto Fukushi
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Sampling Method ◽  
Molecular Detection ◽  
Infectious Bursal Disease ◽  
Tissue Sampling ◽  
Rt Pcr ◽  
Virus Rna ◽  
Bursal Disease

scholarly journals Inhibition of infectious bursal disease virus infection by artificial microRNAs targeting chicken heat-shock protein 90

2012 ◽  
Vol 93 (4) ◽  
pp. 876-879 ◽  
Author(s):  
Weifeng Yuan ◽  
Xinyu Zhang ◽  
Xiaoli Xia ◽  
Huaichang Sun
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Infectious Bursal Disease Virus ◽  
Heat Shock Protein 90 ◽  
Infectious Bursal Disease ◽  
Cellular Receptor ◽  
Receptor Complex ◽  
Rt Pcr ◽  
Transfected Cells ◽  
Bursal Disease

Infectious bursal disease virus (IBDV) causes an important disease in young chickens. Chicken heat-shock protein 90 (cHsp90) has been shown to be a functional component of the cellular receptor complex for IBDV infection. This study demonstrates the inhibitory effect of vector-expressed anti-cHsp90α microRNA (miRNA) on IBDV infection. The reporter vectors pcHsp90α-EGFP and pcHsp90β-EGFP were constructed to facilitate effective miRNA selection. Two anti-cHsp90α and one anti-cHsp90β miRNA-expression vectors were constructed for a stable transfection study. Poly(A)-tailed RT-PCR detected sequence-specific miRNA transcription in transfected cells. Semiquantitative RT-PCR showed inhibition of cHsp90 transcription in transfected cells. A virus-titration assay showed that the anti-cHsp90α miRNA, but not the anti-cHsp90β miRNA, had inhibitory effects on IBDV infection. These results suggest that cHsp90α is a functional component of the cellular receptor complex for IBDV infection, and that anti-cHsp90α miRNA could be used as an anti-IBDV reagent.

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