FimH adhesin from host unrestricted Salmonella Enteritidis binds to different glycoprotein ligands expressed by enterocytes from sheep, pig and cattle than FimH adhesins from host restricted Salmonella Abortus-ovis, Salmonella Choleraesuis and Salmonella Dublin

2013 ◽  
Vol 166 (3-4) ◽  
pp. 550-557 ◽  
Author(s):  
Krzysztof Grzymajło ◽  
Maciej Ugorski ◽  
Rafał Kolenda ◽  
Anna Kędzierska ◽  
Marta Kuźmińska-Bajor ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Salmonella Dublin ◽  
Salmonella Choleraesuis

scholarly journals The immunity produced by a rough Salmonella dublin variant against Salmonella typhimurium and Salmonella choleraesuis infection in guinea-pigs

1966 ◽  
Vol 64 (3) ◽  
pp. 357-359 ◽  
Author(s):  
H. Williams Smith ◽  
Sheila Halls
Keyword(s):  
Salmonella Typhimurium ◽  
Guinea Pigs ◽  
Harmful Effect ◽  
Salmonella Dublin ◽  
Salmonella Choleraesuis

Vaccination with a rough variant of Salmonella dublin had no observable harmful effect on guinea-pigs. It conferred a good immunity against S. typhimurium and S. choleraesuis var kunzendorf infections.

Simultaneous Detection of Multiple Salmonella Serovars from Milk and Chicken Meat by Real-Time PCR Using Unique Genomic Target Regions

2017 ◽  
Vol 80 (11) ◽  
pp. 1944-1957 ◽  
Author(s):  
Sayma Afroj ◽  
Khaled Aldahami ◽  
Gopal Reddy ◽  
Jean Guard ◽  
Abiodun Adesiyun ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Simultaneous Detection ◽  
Taqman Assay ◽  
Comparative Genomic ◽  
Pcr Assay ◽  
Salmonella Dublin ◽  
Milk Samples ◽  
Specificity And Sensitivity ◽  
Salmonella Serovars ◽  
Salmonella Heidelberg

ABSTRACT A novel genomic and plasmid target-based PCR platform was developed for the detection of Salmonella serovars Heidelberg, Dublin, Hadar, Kentucky, and Enteritidis. Unique genome loci were obtained through extensive genome mining of protein databases and comparative genomic analysis of these serovars. Assays targeting Salmonella serovars Hadar, Heidelberg, Kentucky, and Dublin had 100% specificity and sensitivity, whereas those for Salmonella Enteritidis had 97% specificity and 88% sensitivity. The limits of detection for Salmonella serovars Heidelberg, Kentucky, Hadar, Enteritidis, and Dublin were 12, 9, 40, 13, and 5,280 CFU, respectively. A sensitivity assay was also performed by using milk artificially inoculated with pooled Salmonella serovars, yielding a detection limit of 1 to10 CFU/25 mL of milk samples after enrichment. The minimum DNA detected using the multiplexed TaqMan assay was 75.8 fg (1.53 × 101 genomic equivalents [GE]) for Salmonella Heidelberg, 140.8 fg (2.8 × 101 GE) for Salmonella Enteritidis, and 3.48 pg (6.96 × 102 GE) for Salmonella Dublin. PCR efficiencies were 89.8% for Salmonella Heidelberg, 94.5% for Salmonella Enteritidis, and 75.5% for Salmonella Dublin. Four types of 30 pasteurized milk samples were tested negative by culture techniques and with a genus-specific Salmonella invA gene PCR assay. Among 30 chicken samples similarly tested, 12 (40%) were positive by both culture and the invA PCR. Testing of these 12 samples with the serovar-specific PCR assay detected single and mixed contamination with Salmonella Kentucky, Salmonella Enteritidis, and Salmonella Heidelberg. Five unique primers were designed and tested by multiplex conventional PCR in conjunction with the use of the multiplex TaqMan assay with three of the primers. The diagnostic assays developed in this study could be used as tools for routine detection of these five Salmonella serovars and for epidemiological investigations of foodborne disease outbreaks.

scholarly journals Interaction of Salmonella choleraesuis, Salmonella dublin and Salmonella typhimurium with porcine and bovine terminal ileum in vivo

Microbiology ◽  
1999 ◽  
Vol 145 (9) ◽  
pp. 2431-2441 ◽  
Author(s):  
Alex J. Bolton ◽  
Michael P. Osborne ◽  
Tim S. Wallis ◽  
John Stephen
Keyword(s):  
Salmonella Typhimurium ◽  
Terminal Ileum ◽  
Salmonella Dublin ◽  
Salmonella Choleraesuis

scholarly journals Bacteria Isolations from Broiler and Layer Chicks in Zambia

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Hetron Mweemba Munang’andu ◽  
Swithine Hameenda Kabilika ◽  
Oliver Chibomba ◽  
Musso Munyeme ◽  
Geoffrey Munkombwe Muuka
Keyword(s):  
Control Strategy ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Diagnostic Laboratory ◽  
Salmonella Gallinarum ◽  
Breeding Stock ◽  
Salmonella Dublin ◽  
Veterinary Research ◽  
Avian Diseases ◽  
Avian Disease

Chick mortality (CM) is one of the major constraints to the expansion of the poultry industry in Zambia. Of the 2,829 avian disease cases submitted to the national diagnostic laboratory based at the Central Veterinary Research Institute in Lusaka between 1995 and 2007, 34.39% (973/2,829) were from CM cases. The disease accounted for 40.2% (218,787/544,903) mortality in the affected flocks with 89.6% (196,112/218,787) of the affected birds dying within seven days. Major bacteria species involved wereEscherichia coli,Salmonella gallinarum, andProteus speciesbeing isolated from 84.58%, 46.15%, and 26.93% of the reported CM cases (n=973), respectively. Detection ofSalmonella typhimurium,Salmonella enteritidis, andSalmonella dublinindicates that poultry has the potential of transmitting zoonotic pathogenic bacteria to humans. The proportion ofSalmonella gallinarumreactors in the adult breeding stock was generally low (<0.5%) throughout the study period although its prevalence in CM cases was correlated (r=0.68,P<0.011) with seroprevalence of the same pathogen in the adult breeding stock. Given that the disease accounts for a large proportion of the avian diseases in Zambia as shown in the present study (34.39%,n=2,829), it is imperative that an effective disease control strategy aimed at reducing its occurrence should be developed.

Persistence and Growth of Different Salmonella Serovars on Pre- and Postharvest Tomatoes

2007 ◽  
Vol 70 (12) ◽  
pp. 2725-2731 ◽  
Author(s):  
X. SHI ◽  
A. NAMVAR ◽  
M. KOSTRZYNSKA ◽  
R. HORA ◽  
K. WARRINER
Keyword(s):  
Salmonella Enteritidis ◽  
Incubation Temperature ◽  
Narrow Range ◽  
Tomato Fruit ◽  
Diverse Range ◽  
Salmonella Dublin ◽  
Salmonella Newport ◽  
Salmonella Hadar ◽  
Salmonella Serovars ◽  
Salmonella Senftenberg

The interaction of a range of Salmonella serovars with pre- and postharvest tomatoes was evaluated. Serovars were selected on the basis of previous association in tomato-linked outbreaks of salmonellosis (Salmonella Javiana, Salmonella Montevideo, and Salmonella Newport) or those typically isolated from animal or clinical infections (Salmonella Dublin, Salmonella Enteritidis, Salmonella Hadar, Salmonella Infantis, Salmonella Typhimurium, and Salmonella Senftenberg). Salmonella serovars introduced onto the flowers of growing plants were recovered on and within the developing tomato fruit. Of all the Salmonella serovars tested, Montevideo appeared to be more adapted to survival within tomatoes and was recovered from 90% of the fruit screened. All of the Salmonella serovars could persist and grow when introduced onto unripened (green) tomato fruit. In general, growth (internal and external) was promoted at the high incubation temperature (25°C) and high relative humidity (95%), although this was serovar dependent. The growth and persistence of Salmonella introduced on and into ripened (red) tomatoes was serovar dependent. Salmonella serovars Enteritidis, Typhimurium, and Dublin were less adapted to grow in or on intact red tomatoes than were serovars Hadar, Montevideo, or Newport. The results illustrated that a diverse range of Salmonella serovars can become established within and/or on preharvest tomatoes. The majority of Salmonella can grow and become established both on and within unripened tomatoes, but growth on ripened fruit was serovar dependent. The results provide a possible explanation why only a narrow range of Salmonella serovars are associated with foodborne illness outbreaks linked to tomatoes.

scholarly journals Caracterização de sorotipos em linhagens do gênero Salmonella isoladas de diferentes afecções em animais domésticos

2010 ◽  
Vol 30 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Márcio G. Ribeiro ◽  
Marta C. Fernandes ◽  
Antonio C. Paes ◽  
Amanda K. Siqueira ◽  
José P.A.N. Pinto ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Salmonella Enteritidis ◽  
Salmonella Dublin ◽  
Salmonella Newport

Foram caracterizados os sorotipos, o perfil de sensibilidade microbiana e os achados clínico-epidemiológicos em 53 linhagens do gênero Salmonella isoladas de 41 cães, nove equinos e três bovinos, acometidos por diferentes manifestações clínicas entre 1997 e 2007. Salmonella Typhimurium (45,3%), Salmonella enterica (22,6%), Salmonella Enteritidis (7,5%), Salmonella enterica subsp enterica 4,5,12i (5,7%), Salmonella Newport (5,7%), Salmonella Dublin (3,8%), Salmonella Agona (3,8%), Salmonella Glostrup (3,8%), Salmonella Saintpaul (1,8%) foram os sorotipos encontrados. Ciprofloxacina (100,0%), norfloxacina (100,0%) e gentamicina (100,0%) foram os antimicrobianos mais efetivos, enquanto a maior resistência das linhagens foi observada para ceftiofur (28,5%) e florfenicol (7,0%). As linhagens foram isoladas de animais com enterite, infecção do trato urinário, septicemia, piometra, pneumonia e conjuntivite. Ressalta-se para o predomínio do sorovar Typhimurium nas diferentes manifestações da salmonelose nos animais. Destaca-se, também, a identificação de sorotipos nos animais que também são observados em casos de salmonelose em humanos

Activation of isolated heterophils from chicks stimulated in vivo with salmonella enteritidis-immune lymphokines

1996 ◽  
Vol 54 ◽  
pp. 760-761
Author(s):  
R. B. Moyes ◽  
R. E. Droleskey ◽  
M. H. Kogut ◽  
J. R. DeLoach
Keyword(s):  
Lamina Propria ◽  
Intestinal Mucosa ◽  
Salmonella Enteritidis ◽  
Intestinal Tract ◽  
Polymorphonuclear Cells ◽  
Subclinical Infection ◽  
Egg Products ◽  
Immune Lymphokines ◽  
Organ Invasion

Salmonella enteritidis (SE) is of great concern to the poultry industry due to the organism's ability to penetrate the intestinal mucosa of the laying hen and subsequently colonize the ovaries and yolk membrane. The resultant subclinical infection can lead to SE infection of raw eggs and egg products. Interference with the ability of the organism to invade has been linked to the activation and recruitment of inflammatory polymorphonuclear cells, heterophils, to the lamina propria of the intestinal tract.Recently it has been established that heterophil activation and increased resistance to SE organ invasion can be accomplished by the administration of SE-immune lymphokines (SE-ILK) obtained from supernatants of concanavalin-A stimulated SE immune T lymphocytes from SE hyperimmunized hens. Invasion of SE into the lamina propria provides a secondary signal for directing activated heterophils to the site of SE invasion.

Sweet's syndrome associated with Salmonella enteritidis infection

1999 ◽  
Vol 24 (3) ◽  
pp. 239-240 ◽  
Author(s):  
Flórez ◽  
Sánchez-Aguilar ◽  
Rosón ◽  
Prieto ◽  
Van den Eyden ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Sweet’S Syndrome ◽  
Sweet's Syndrome
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