Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil

2012 ◽  
Vol 163 (2) ◽  
pp. 667-671 ◽  
Author(s):  
A. Henzel ◽  
M. Sá e Silva ◽  
S. Luo ◽  
L.T. Lovato ◽  
R. Weiblen
Keyword(s):  
Capsid Protein ◽  
Protein Gene ◽  
Phylogenetic Analyses ◽  
Rio Grande ◽  
Feline Calicivirus ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Capsid Protein Gene

scholarly journals Detection and Phylogenetic Analyses of Taura Syndrome Virus from Archived Davidson’s-Fixed Paraffin-Embedded Shrimp Tissue

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1030 ◽  
Author(s):  
Lauren Marie Ochoa ◽  
Roberto Cruz-Flores ◽  
Arun K. Dhar
Keyword(s):  
Capsid Protein ◽  
Protein Gene ◽  
Rna Virus ◽  
Phylogenetic Analyses ◽  
Animal Health ◽  
Disease Spread ◽  
Diagnostic Tools ◽  
Taura Syndrome Virus ◽  
Capsid Protein Gene ◽  
Geographical Regions

Taura syndrome is a World Organization for Animal Health (OIE)-listed disease of marine shrimp that is caused by Taura syndrome virus (TSV), a single-stranded RNA virus. Here we demonstrate the utility of using 15-year-old archived Davidson’s-fixed paraffin-embedded (DFPE) shrimp tissues for TSV detection and phylogenetic analyses. Total RNA was isolated from known TSV-infected DFPE tissues using three commercially available kits and the purity and ability to detect TSV in the isolated RNA were compared. TSV was successfully detected through RT-qPCR in all the tested samples. Among the TSV-specific primers screened through RT-PCR, primer pair TSV-20 for the RNA-dependent RNA polymerase (RdRp), primers TSV-15 and TSV-16 for the capsid protein gene VP2 and primers TSV-5 for the capsid protein gene VP1 amplified the highest number of samples. To assess the phylogenetic relation among different TSV isolates, the VP1 gene was amplified and sequenced in overlapping segments. Concatenated sequences from smaller fragments were taken for phylogenetic analyses. The results showed that the TSV isolates from this study generally clustered with homologous isolates from the corresponding geographical regions indicating RNA derived from DFPE tissues can be used for pathogen detection and retrospective analyses. The ability to perform genomic characterization from archived tissue will expedite pathogen discovery, development of diagnostic tools and prevent disease spread in shrimp and potentially other aquaculture species worldwide.

scholarly journals Identification and sequence determination of the capsid protein gene of feline calicivirus

1992 ◽  
Vol 122 (3-4) ◽  
pp. 223-235 ◽  
Author(s):  
M. J. Carter ◽  
I. D. Milton ◽  
P. C. Turner ◽  
J. Meanger ◽  
M. Bennett ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Protein Gene ◽  
Feline Calicivirus ◽  
Sequence Determination ◽  
Capsid Protein Gene

Tropicoporus stratificans sp. nov. (Hymenochaetales, Basidiomycota) from southern Brazil

Phytotaxa ◽  
2016 ◽  
Vol 245 (2) ◽  
pp. 144 ◽  
Author(s):  
Gilberto Coelho ◽  
ANDRESSA DE OLIVEIRA SILVEIRA ◽  
ZAIDA INÊS ANTONIOLLI ◽  
EUGENE YURCHENKO
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
Phylogenetic Analyses ◽  
Wood Decay ◽  
Rio Grande ◽  
New Taxon ◽  
Nrdna Its ◽  
Decayed Wood ◽  
Southern Brazil ◽  
Rio Grande Do Sul

Tropicoporus is a recently proposed genus that was segregated from Inonotus s.l. and includes species with important wood decay and medicinal functions. The description of T. stratificans sp. nov. is based on more than 10 specimens collected on fallen decayed wood in subtropical forests of Rio Grande do Sul State. The species is recognized by its resupinate, poroid, golden brown, perennial basidiomes that become multi-layered, dimitic hyphal system, abundant hymenial setae, and subglobose to ellipsoid, brownish-yellow to ferruginous-brown basidiospores. Based on nrDNA ITS sequences, Bayesian and maximum likelihood phylogenetic analyses of Tropicoporus nested the new taxon in the T. linteus clade. A description and illustrations of the new species are provided.

A 12-year study of fungal infections in Rio Grande do Sul, Southern Brazil

2019 ◽  
Vol 36 (2) ◽  
pp. 55-60 ◽  
Author(s):  
Vanessa da Silva Fay ◽  
Tatiana Schäffer Gregianini ◽  
Ana Beatriz Gorini da Veiga ◽  
Stela Maris Bottin Gonçalves ◽  
Diana Mara Rodrigues ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Rio Grande ◽  
Southern Brazil ◽  
Rio Grande Do Sul

Molecular Detection of Trypanosoma kaiowa in Tabanus triangulum (Diptera: Tabanidae) from the Coastal Plain of Rio Grande do Sul, Southern Brazil

2021 ◽  
Author(s):  
Gratchela D. Rodrigues ◽  
Eduardo Blodorn ◽  
Ândrio Zafalon-Silva ◽  
William Domingues ◽  
Roberta Marques ◽  
...  
Keyword(s):  
Coastal Plain ◽  
Molecular Detection ◽  
Rio Grande ◽  
Southern Brazil ◽  
Rio Grande Do Sul

scholarly journals Habitat use patterns of franciscana dolphins (Pontoporia blainvillei) off southern Brazil in relation to water depth

2009 ◽  
Vol 89 (5) ◽  
pp. 943-949 ◽  
Author(s):  
Daniel Danilewicz ◽  
Eduardo R. Secchi ◽  
Paulo H. Ott ◽  
Ignacio B. Moreno ◽  
Manuela Bassoi ◽  
...  
Keyword(s):  
Habitat Use ◽  
Rio Grande ◽  
Limiting Factors ◽  
Small Scale ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Use Patterns ◽  
Management Procedures ◽  
By Catch ◽  
Pontoporia Blainvillei

The patterns of habitat use by the franciscana dolphins (Pontoporia blainvillei) along its distribution are poorly known. This study investigates the patterns of habitat use with respect to depth for 181 individuals of different age, size, gender and reproductive condition off Rio Grande do Sul, southern Brazil. The results reveal that franciscanas are very homogeneously distributed according to depth. Individuals from all lengths utilize nearly the entire range of depths of the surveyed area. Larger or older animals do not use deeper waters than younger animals, indicating that body size and age are not limiting factors for franciscanas that occupy deeper or offshore waters. Gestation seems to not cause a change in the distribution of females. Although the sex-ratio of the overall data did not vary from 1:1 in different depth intervals, a small-scale comparison between the northern and southern coast demonstrated the existence of some kind of sexual segregation. Franciscana by-catch in Rio Grande do Sul is not sustainable and it is agreed that management procedures are needed. Nevertheless, the available data on species distribution do not allow the designing of a protected area in order to minimize the by-catches of a particular sex/reproductive class.

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