Comment on “Modelling the PAO–GAO competition: Effects of carbon source, pH and temperature” by Lopez-Vazquez, C.M., Oehmen, A., Hooijmans, C.M., Brdjanovic, D., Gijzen, H.J., Yuan, Z., van Loosdrecht, M.C.M. Water Res. (2009)

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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Metabolic flux analysis of Escherichia coli K-12 based on 13C-labeling experiments with measurement of intracellular metabolite concentrations for carbon source-limited and nitrogen source-limited chemostat cultures

Metabolic Engineering ◽

10.1016/s1096-7176(03)00045-4 ◽

2003 ◽

Author(s):

J Zhu

Keyword(s):

Escherichia Coli ◽

Carbon Source ◽

Nitrogen Source ◽

Metabolic Flux Analysis ◽

Metabolic Flux ◽

Flux Analysis ◽

Intracellular Metabolite ◽

Chemostat Cultures ◽

Metabolite Concentrations ◽

K 12

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Research on the expansion mode of the spatial structure of the land used for carbon source and sink at urban fringe areas: a case of Laibin city

Advanced Civil, Urban and Environmental Engineering ◽

10.2495/cuee140391 ◽

2015 ◽

Author(s):

J. Zhao ◽

M. L. Qin ◽

J. Ya ◽

H. Li

Keyword(s):

Carbon Source ◽

Spatial Structure ◽

Urban Fringe ◽

Source And Sink

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Utilization of sulfate-reducing bacteria with fermented soybeans as a carbon source for the removal of sulfate in acidic wastewaters

Advances in Earth and Environmental Sciences ◽

10.2495/icesep130971 ◽

2013 ◽

Author(s):

Guijuan Ji ◽

Guoyan Zhang ◽

Shujie Zhao ◽

Ning Li ◽

Yuguo Cui ◽

...

Keyword(s):

Carbon Source ◽

Sulfate Reducing Bacteria ◽

Sulfate Reducing ◽

Reducing Bacteria

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Enantioselective Multi-Component Cyclopropane Synthesis Enabled by Cu-Catalyzed Cyclopropene Carbometallation with Organoboron Reagent

10.26434/chemrxiv.7011548 ◽

2018 ◽

Author(s):

Zhanyu Li ◽

Mengru Zhang ◽

Yu Zhang ◽

Shuang Liu ◽

Jinbo Zhao ◽

...

Keyword(s):

Carbon Source ◽

Stereoselective Synthesis ◽

Organometallic Reagents ◽

Preliminary Results ◽

Quaternary Center ◽

Chiral Centers ◽

Substituted Cyclopropanes ◽

Proof Of Principle

Deployment of organoboron in lieu of the strongly basic organometallic reagents as carbon source in Cu-catalyzed cyclopropene carbometallation opens unprecedented three- component reactivity for stereoselective synthesis of poly-substituted cyclopropanes. A proof-of-principle demonstration of this novel carbometallation strategy is presented herein for a highly convergent access to poly-substituted aminocyclopropane framework via carboamination. Preliminary results on asymmetric desymmetrization with commercial bisphosphine ligands attained high levels of enantioselection, offering a straightforward access to enantioenriched aminocyclopropanes bearing all-three chiral centers, including an all-carbon quaternary center. This strategy may underpin a host of novel synthetic protocols for poly-substituted cyclopropanes.

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