A transgenic reporter under control of an es1 promoter/enhancer marks wound epidermis and apical epithelial cap during tail regeneration in Xenopus laevis tadpole

Unlike mammals, Xenopus laevis tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key regenerative pathways, signaling to progenitors to reconstitute lost tissue. These findings reveal the cellular mechanism through which ROCs form the wound epidermis and ensure successful regeneration.

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Patterns of Mitosis and Activation of the Map-Kinase Cascade during Tadpole Tail Regeneration in the Refractory Period of Xenopus laevis Development

Russian Journal of Developmental Biology ◽

10.1134/s1062360418050028 ◽

2018 ◽

Vol 49 (5) ◽

pp. 260-263

Author(s):

A. S. Ivanova ◽

G. V. Ermakova ◽

A. G. Zaraisky ◽

M. B. Tereshina

Keyword(s):

Xenopus Laevis ◽

Map Kinase ◽

Refractory Period ◽

Tail Regeneration ◽

Map Kinase Cascade ◽

Tadpole Tail ◽

Kinase Cascade

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Expression of the Ca2+-binding protein, parvalbumin, during embryonic development of the frog, Xenopus laevis

The Journal of Cell Biology ◽

10.1083/jcb.104.4.841 ◽

1987 ◽

Vol 104 (4) ◽

pp. 841-847 ◽

Cited By ~ 17

Author(s):

BK Kay ◽

AJ Shah ◽

WE Halstead

Keyword(s):

Xenopus Laevis ◽

Fusion Protein ◽

Binding Protein ◽

Expression Library ◽

Simple Method ◽

High Affinity ◽

Tadpole Tail ◽

Developing Muscle ◽

Beta Galactosidase ◽

Xenopus Laevis Tadpole

A cDNA segment encoding the Ca2+-binding protein, parvalbumin, was isolated with the use of antibodies, from a lambda gtll expression library of Xenopus laevis tadpole poly(A)+ RNAs. The bacterially expressed beta-galactosidase-parvalbumin fusion protein of one lambda recombinant shows high affinity 45Ca2+ binding. The sequence of the tadpole parvalbumin is highly similar to previously characterized beta-parvalbumins of other organisms. Data from protein and RNA blotting experiments demonstrate that parvalbumin is absent in oocytes, eggs, and early staged embryos, and only becomes expressed during embryogenesis at the time of myogenesis. The protein can be detected in individual developing muscle cells and in muscle fibers of tadpole tail muscles. A simple method is also described for the isolation of neural tube-notochord-somite complexes from Xenopus embryos.

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