Influence of down-regulation of caspase-3 by siRNAs on sodium-butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin

2005 ◽  
Vol 7 (5-6) ◽  
pp. 457-466 ◽  
Author(s):  
Yun Hee Sung ◽  
Su-Jeong Hwang ◽  
Gyun Min Lee
Keyword(s):  
Cell Death ◽  
Sodium Butyrate ◽  
Chinese Hamster Ovary ◽  
Caspase 3 ◽  
Apoptotic Cell ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Apoptotic Cell Death ◽  
Down Regulation ◽  
Ovary Cells

scholarly journals Co-expression of MG29 and Ryanodine Receptor Leads to Apoptotic Cell Death

2004 ◽  
Vol 279 (19) ◽  
pp. 19387-19390 ◽  
Author(s):  
Zui Pan ◽  
Yutaka Hirata ◽  
Ramakrishnan Y. Nagaraj ◽  
Jiying Zhao ◽  
Miyuki Nishi ◽  
...  
Keyword(s):  
Cell Death ◽  
Ryanodine Receptor ◽  
Apoptotic Cell ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Bilayer Membrane ◽  
Apoptotic Cell Death ◽  
Functional Interaction ◽  
Release Channel ◽  
Intracellular Ca

Perturbation of intracellular Ca2+homeostasis has been shown to regulate the process of cell proliferation and apoptosis. Our previous studies show that mitsugumin 29 (MG29), a synaptophysin-related protein localized in the triad junction of skeletal muscle, serves an essential role in muscle Ca2+signaling by regulating the process of store-operated Ca2+entry. Here we report a functional interaction between MG29 and the ryanodine receptor (RyR)/Ca2+release channel. The purified MG29 protein enhances activity of the RyR/Ca2+release channel incorporated into the lipid bilayer membrane. Co-expression of MG29 and RyR in Chinese hamster ovary cells leads to apoptotic cell death resulting from depletion of intracellular Ca2+stores, despite neither protein expression alone exhibits any significant effect on cell viability. In transient expression studies, the presence of RyR in the endoplasmic reticulum leads to retention of MG29 from the plasma membrane into the intracellular organelles. This functional interaction between MG29 and RyR could have important implications in the Ca2+signaling processes of muscle cells. Our data also show that perturbation of intracellular Ca2+homeostasis can serve as a key signal in the initiation of apoptosis.

Sign in / Sign up

Export Citation Format

Share Document