High-pressure NMR spectroscopy for characterizing folding intermediates and denatured states of proteins

Methods ◽  
2004 ◽  
Vol 34 (1) ◽  
pp. 133-143 ◽  
Author(s):  
Y KAMATARI
Keyword(s):  
High Pressure ◽  
Nmr Spectroscopy ◽  
Folding Intermediates ◽  
Denatured States

Intermolecular Interactions of Xe Atoms Confined in One-dimensional Nanochannels of Tris(o-phenylenedioxy)cyclotriphosphazene as Studied by High-pressure 129Xe NMR

2003 ◽  
Vol 58 (12) ◽  
pp. 727-734 ◽  
Author(s):  
Hirokazu Kobayashi ◽  
Takahiro Ueda ◽  
Keisuke Miyakubo ◽  
Taro Eguchi
Keyword(s):  
High Pressure ◽  
Nmr Spectroscopy ◽  
Chemical Shift ◽  
Pressure Dependence ◽  
Average Distance ◽  
Chemical Shift Tensor ◽  
Axially Symmetric ◽  
One Dimensional ◽  
129Xe Nmr ◽  
The One

The pressure dependence of the 129Xe chemical shift tensor confined in the Tris(o-phenylenedioxy) cyclotriphosphazene (TPP) nanochannel was investigated by high-pressure 129Xe NMR spectroscopy. The observed 129Xe spectrum in the one-dimensional TPP nanochannel (0.45 nm in diameter) exhibits a powder pattern broadened by an axially symmetric chemical shift tensor. As the pressure increases from 0.02 to 7.0 MPa, a deshielding of 90 ppm is observed for the perpendicularcomponent of the chemical shift tensor δ⊥, whereas a deshielding of about 30 ppm is observed for the parallel one, δ‖. This suggests that the components of the chemical shift tensor, δ‖ and δ⊥, are mainly dominated by the Xe-wall and Xe-Xe interaction, respectively. Furthermore, the effect of helium, which is present along with xenon gas, on the 129Xe chemical shift is examined in detail. The average distance between the Xe atoms in the nanochannel is estimated to be 0.54 nm. This was found by using δ⊥ at the saturated pressure of xenon, and comparing the increment of the chemicalshift value in δ⊥ to that of a β -phenol/Xe compound.

scholarly journals Monitoring Unfolding of Titin I27 Single and Bi Domain with High-Pressure NMR Spectroscopy

2018 ◽  
Vol 115 (2) ◽  
pp. 341-352 ◽  
Author(s):  
Isaline Herrada ◽  
Philippe Barthe ◽  
Marisa Vanheusden ◽  
Karine DeGuillen ◽  
Léa Mammri ◽  
...  
Keyword(s):  
High Pressure ◽  
Nmr Spectroscopy

scholarly journals Species-specific Differences in the Intermediate States of Human and Syrian Hamster Prion Protein Detected by High Pressure NMR Spectroscopy

2007 ◽  
Vol 282 (31) ◽  
pp. 22689-22698 ◽  
Author(s):  
Werner Kremer ◽  
Norman Kachel ◽  
Kazuo Kuwata ◽  
Kazuyuki Akasaka ◽  
Hans Robert Kalbitzer
Keyword(s):  
High Pressure ◽  
Nmr Spectroscopy ◽  
Prion Protein ◽  
Syrian Hamster ◽  
Intermediate States ◽  
Species Specific
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