Ultrastructure of the sporozoite and the sporocyst wall of the coccidian species Adelina triboliiBhatia, 1937 within the course of an autoinfection

A parasitological survey was conducted at some zoos in the states of São Paulo and Paraná, Brazil, from 2009 to 2011. Several groups of birds were surveyed for fecal samples, but the most important was Psittacidae. Among the parasites, Eimeria (coccidian) and Capillaria, Ascaridia and Heterakis (nematodes) were observed in almost one third of the samples. Presence of a rich parasite fauna associated with captive birds seems to be an effect of captivity, since data on free-ranging birds indicate few or virtually no parasites at all. The discovery of new coccidian species during this survey reveals the need of more research on the subject as even well-known bird species have unknown parasites, but caution must be exercised in order to avoid descriptions of pseudoparasites.

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Isospora troglodytes n. sp. (Apicomplexa: Eimeriidae), a new coccidian species from wrens of Costa Rica

Parasitology Research ◽

10.1007/s00436-011-2691-y ◽

2011 ◽

Vol 110 (5) ◽

pp. 1723-1725 ◽

Cited By ~ 3

Author(s):

Shamus P. Keeler ◽

Michael J. Yabsley ◽

Julie M. Fox ◽

Sabrina N. McGraw ◽

Sonia M. Hernandez

Keyword(s):

Costa Rica ◽

Coccidian Species

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The ruby-crowned tanager Tachyphonus coronatus Vieillot, 1822 (Passeriformes: Thraupidae) as a new host for Isospora ramphoceli Berto, Flausino, Luz, Ferreira, Lopes, 2010 in Brazil

Acta Parasitologica ◽

10.1515/ap-2017-0038 ◽

2017 ◽

Vol 62 (2) ◽

Author(s):

Mariana Borges Rodrigues ◽

Irlane Faria de Pinho ◽

Lidiane Maria da Silva ◽

Bruno doBomfim Lopes ◽

Hermes Ribeiro Luz ◽

...

Keyword(s):

Protected Area ◽

Current Work ◽

Southeastern Brazil ◽

New Host ◽

Parque Nacional Do Itatiaia ◽

Coccidian Species ◽

High Degree

AbstractDespite 12 coccidian species had been recorded from passerines of the Thraupidae family, none of them has been reported in the Parque Nacional do Itatiaia, in Southeastern Brazil. This locality is a protected area with a high degree of vulnerability, and is considered a "conservation island" of biodiversity. The aim of the current work was describe

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Isospora machadoae sp. nov. (Protozoa: Apicomplexa: Eimeriidae), a new coccidian species from white-necked thrushes Turdus albicolis (Passeriformes: Turdidae) of South America

Zoologia (Curitiba) ◽

10.3897/zoologia.35.e24570 ◽

2018 ◽

Vol 35 ◽

pp. 1-4 ◽

Cited By ~ 1

Author(s):

Irlane F. de Pinho ◽

Lidiane M. da Silva ◽

Mariana B. Rodrigues ◽

Bruno do B. Lopes ◽

Mariana S. Oliveira ◽

...

Keyword(s):

South America ◽

Clinical Signs ◽

Refractile Body ◽

Southeastern Brazil ◽

Ecological Niches ◽

Sporocyst Residuum ◽

Polar Granules ◽

Parque Nacional Do Itatiaia ◽

Coccidian Species ◽

Oocyst Residuum

A new coccidian species parasitizing white-necked thrushes Turdus albicollis Vieillot, 1818 is described from the Parque Nacional do Itatiaia, in Southeastern Brazil. Isospora machadoae sp. nov. has oocysts that are sub-spherical, 22.2 × 21.2 µm, with bilayered wall, ~1.3 μm thick. Outer layer is rough with micropyle and micropyle cap. Oocyst residuum is absent, but one or two polar granules are present. Sporocysts are ellipsoidal, 13.3 × 9.7 µm. The Stieda body is flattened to half-moon-shaped and substieda body rounded. Sporocyst residuum is present, composed of scattered spherules of different sizes. Sporozoites are vermiform with a refractile body and a nucleus. These parasitized thrushes had no apparent clinical signs of coccidiosis or high densities of oocysts in feces. This condition may be associated with a specific low pathogenicity of I. machadoae sp. nov. and/or with the conserved habitat of these birds, which ensures the ecological niches and thus the immunocompetence to wildlife.

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Effect of household bleach on the structure of the sporocyst wall of Toxoplasma gondii

Parasite ◽

10.1051/parasite/2021066 ◽

2021 ◽

Vol 28 ◽

pp. 68

Author(s):

Aurélien Dumètre ◽

Jitender P. Dubey ◽

David J.P. Ferguson

Keyword(s):

Toxoplasma Gondii ◽

Outer Layer ◽

Wall Layer ◽

Cross Link ◽

Oocyst Wall ◽

Maclura Pomifera ◽

Transmission Electron ◽

Household Bleach ◽

Sporocyst Wall ◽

Molecular Nature

Toxoplasma gondii oocysts are responsible for food- and water-borne infections in humans worldwide. They are resistant to common chemical disinfectants, including chlorinated products, presumably due to the structure and molecular nature of the oocyst wall but also the sporocyst wall. In this study, we used fluorescence microscopy and transmission electron microscopy to characterise the structure of both the oocyst and sporocyst walls, exposed to household bleach. Bleach removed the outer layer of the oocyst wall and the outer layer of the wall of sporocysts exposed due to rupture of the oocyst wall. The loss of the outer sporocyst wall layer was associated with a decrease in its autofluorescence, which can be linked to the degradation of dityrosine cross-link proteins, and loss of Maclura pomifera lectin-reactive glycoproteins. This study suggests that the inner layers of the oocyst and sporocyst walls are the main structures responsible for the resistance of the parasite to household bleach.

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Isospora ramphoceli n. sp. (Apicomplexa: Eimeriidae) from the Brazilian tanager (Aves: Passeriformes: Thraupidae) Ramphocelus bresilius dorsalis Sclater, 1855

Zootaxa ◽

10.11646/zootaxa.2650.1.5 ◽

2010 ◽

Vol 2650 (1) ◽

pp. 57 ◽

Cited By ~ 4

Author(s):

BRUNO PEREIRA BERTO ◽

WALTER FLAUSINO ◽

HERMES RIBEIRO LUZ ◽

ILDEMAR FERREIRA ◽

CARLOS WILSON GOMES LOPES

Keyword(s):

Polar Granule ◽

Refractile Body ◽

Stieda Body ◽

Sporocyst Residuum ◽

Coccidian Species ◽

Oocyst Residuum

In the current study, a new coccidian species (Protozoa: Apicomplexa: Eimeriidae) collected from the Brazilian tanager Ramphocelus bresilius dorsalis Sclater, 1855 is reported from Brazil. Isospora ramphoceli n. sp. has oocysts which are spherical to sub-spherical, 23.7 × 22.8 μ m, with smooth, bilayered wall, ~1.2 μ m thick. Micropyle, oocyst residuum and polar granule are absent. Sporocysts are ellipsoidal or slightly ovoidal, 16.0 × 11.4 µm. Stieda body knob-like and prominent and substieda body large and homogeneous. Sporocyst residuum is composed of granules of different sizes. Sporozoites are vermiform with one refractile body and a nucleus. This is the sixth description of an isosporoid coccidium infecting R. b. dorsalis and the twelfth description from Thraupidae.

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Transformation of oocysts from several coccidian species by heat treatment

Zeitschrift für Parasitenkunde Parasitology Research ◽

10.1007/bf00931810 ◽

1989 ◽

Vol 75 (4) ◽

pp. 264-267 ◽

Cited By ~ 7

Author(s):

T. Matsui ◽

T. Morii ◽

T. Iijima ◽

F. Kobayashi ◽

T. Fujino

Keyword(s):

Heat Treatment ◽

Coccidian Species

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Ultrastructure of the life-cycle stages of Goussia janae (Apicomplexa, Eimeriidae), with X-ray microanalysis of accompanying precipitates

Canadian Journal of Zoology ◽

10.1139/z92-320 ◽

1992 ◽

Vol 70 (12) ◽

pp. 2382-2397 ◽

Cited By ~ 5

Author(s):

Julius Lukeš ◽

Vladimír Starý

Keyword(s):

Life Cycle ◽

Host Cell ◽

Intestinal Epithelium ◽

Parasitophorous Vacuole ◽

Oocyst Wall ◽

Cell Cytoplasm ◽

X Ray ◽

Host Cell Cytoplasm ◽

Life Cycle Stages ◽

Sporocyst Wall

The ultrastructural features of the life-cycle stages of Goussia janae from the intestinal epithelium of the dace Leuciscus leuciscus and chub L. cephalus are described. All merogonial, gamogonial, and early sporogonial stages were localized in the microvillar region in an intracellular and extracytoplasmic position, covered by closely apposed enterocyte and parasitophorous vacuole membranes. Two types of location in the host cell were observed: (i) a more frequent "monopodial" type with a single zone of attachment to the host-cell cytoplasm, and (ii) a "spider-like" type with several isolated zones of attachment. Merozoites were formed by either ecto- or endo-merogony. Microgamonts produced elongated biflagellate microgametes at their periphery. The oocyst wall, produced exclusively by the parasite, was formed at the end of the intracellular phase of the life cycle. Exogenous sporulation resulted in the formation of elongated sporocysts with a thin sporocyst wall bearing a longitudinal suture accompanied by a narrow membranaceous veil. In the cytoplasm and cytoplasmic and parasitophorous vacuoles of the parasite, fine, dense precipitates were present. X-ray microanalysis of these precipitates from osmicated and non-osmicated samples revealed high levels of Ca and P, indicating the possible presence of hydroxyapatite.

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