Orexin A-like immunoreactivity in the hypothalamus and thalamus of the Syrian hamster (Mesocricetus auratus) and Siberian hamster (Phodopus sungorus), with special reference to circadian structures

SummaryThe Deleted in AZoospermia (DAZ) gene family regulates the development, maturation and maintenance of germ cells and spermatogenesis in mammals. The DAZ family consists of two autosomal genes, Boule and Dazl (Daz-like), and the Daz gene on chromosome Y. The aim of this study was to analyze the localization of DAZL and BOULE during testicular ontogeny of the seasonal-breeding Syrian hamster, Mesocricetus auratus. We also evaluated the testicular expression of DAZ family genes under short- or long-photoperiod conditions. In the pre-pubertal and adult testis, DAZL protein was found mainly in spermatogonia. BOULE was found in the spermatogonia from 20 days of age and during the pre-pubertal and adult period it was also detected in spermatocytes and round spermatids. DAZL and BOULE expression in spermatogonia was strictly nuclear only in 20-day-old hamsters. We also detected the novel mRNA and protein expression of BOULE in Leydig cells. In adult hamsters, Dazl expression was increased in regressed testis compared with non-regressed testis and DAZL protein expression was restricted to primary spermatocytes in regressed testis. These results show that DAZL and BOULE are expressed in spermatogonia at early stages in the Syrian hamster, then both proteins translocate to the cytoplasm when meiosis starts. In the adult regressed testis, the absence of DAZL in spermatogonia might be related to the decrease in germ cell number, suggesting that DAZ gene family expression is involved in changes in seminiferous epithelium during photoregression.

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Photoperiodic and ovarian steroid regulation of histone deacetylase 1 , 2 , and 3 in Siberian hamster ( Phodopus sungorus ) reproductive tissues

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2016.12.008 ◽

2017 ◽

Vol 246 ◽

pp. 194-199 ◽

Cited By ~ 6

Author(s):

Eloise W.J. Lynch ◽

Christopher S. Coyle ◽

Tyler J. Stevenson

Keyword(s):

Histone Deacetylase ◽

Phodopus Sungorus ◽

Ovarian Steroid ◽

Siberian Hamster ◽

Histone Deacetylase 1 ◽

Reproductive Tissues ◽

Steroid Regulation

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Spontaneous glomerular basement membrane changes in the golden Syrian hamster (Mesocricetus auratus): a light and electron microscope study

Laboratory Animals ◽

10.1258/002367778781088530 ◽

1978 ◽

Vol 12 (4) ◽

pp. 207-211 ◽

Cited By ~ 5

Author(s):

E. A. E. Van Marck ◽

W. Jacob ◽

A. M. Deelder ◽

P. L. J. Gigase

Keyword(s):

Electron Microscope ◽

Basement Membrane ◽

Glomerular Basement Membrane ◽

Syrian Hamster ◽

Microscope Study ◽

Electron Microscope Study ◽

Mesocricetus Auratus ◽

Golden Syrian Hamster ◽

Membrane Changes ◽

Apparently Healthy

Changes in apparently healthy hamsters, consistent with proteinuria, are reported, but no IgG deposits or amyloid in the glomeruli were detected. Further investigation is required into the significance and the aetiology of these, as yet, obscure alterations.

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