Anti-IgG type test to assay circulating immune complexes from polyethylene glycol precipitates compared with C1q binding and Raji cell tests

1984 ◽  
Vol 17 (6) ◽  
pp. 341-344 ◽  
Author(s):  
Stanley S. Levinson ◽  
Janice Goldman
Keyword(s):  
Polyethylene Glycol ◽  
Immune Complexes ◽  
Raji Cell ◽  
Circulating Immune Complexes ◽  
Type Test

Erroneous results with routine laboratory testing for immunoglobulins due to interference from circulating immune complexes in a case of hyperviscosity syndrome associated with autoimmune disease.

1988 ◽  
Vol 34 (4) ◽  
pp. 784-787 ◽  
Author(s):  
S S Levinson ◽  
M Perry ◽  
J Goldman ◽  
L E Nathan
Keyword(s):  
Polyethylene Glycol ◽  
Immune Complexes ◽  
Accurate Determination ◽  
Raji Cell ◽  
Circulating Immune Complexes ◽  
Binding Assays ◽  
Hyperviscosity Syndrome ◽  
Rheumatoid Factor Activity ◽  
High Concentrations ◽  
Very High

Abstract A patient with the hyperviscosity syndrome exhibited very high concentrations of intermediate to small circulating immune complexes (CIC), involving 40-50% of the IgG present, with IgG rheumatoid factor activity. We demonstrate that precipitation of CIC by polyethylene glycol in the reaction mixture caused interference with nephelometric methods for measuring IgM and IgA, and that failure of immunoglobulins to migrate, owing to molecular interactions, caused interference with radial immunodiffusion methods. Semiquantitative values for immunoglobulins were difficult to interpret on immunoelectrophoresis. As a result, IgM and IgA could only be quantitatively estimated by an end-point nephelometric approach that included a serum blank. Immunoelectrophoresis indicated that a large proportion of the immunoglobulins behaved as aggregates. Immunofixation electrophoresis did not reveal the presence of aggregates. The polyethylene glycol-IgG test provided an accurate assessment of the CIC concentration; the Raji cell and C1q-binding assays did not. Evidently, special techniques may be necessary for accurate determination of immunoglobulin concentrations when CIC concentrations are very high.

scholarly journals Influence of Melatonin on the state of immunity at psoriasis

2009 ◽  
Vol 8 (4) ◽  
pp. 148-151
Author(s):  
V. S. Dmitruk ◽  
L. V. Striga
Keyword(s):  
Immune System ◽  
Polyethylene Glycol ◽  
Immune Complexes ◽  
Combined Treatment ◽  
Circulating Immune Complexes ◽  
Selective Precipitation ◽  
Exogenous Melatonin ◽  
Immunomodulatory Action ◽  
Immunofluorescent Method

The influence of the chronopharmacological scheme of treatment with the use of exogenous Melatonin in the form of the Melaksen drug on the immunity status of psoriasis patients has been studied. The study of the immunity status consisted in determination of the main Manchini classes of immunoglobulins A, M, G, the level of circulating immune complexes by the method of selective precipitation in 3% polyethylene glycol. In addition, we determined the subpopulation of lymphocytes carrying CD3, CD4, CD8, CD16, CD 25, and CD72 antigens by the immunofluorescent method. In has been found that Melatonin in psoriasis patients exerts the immunomodulatory action on the immune system at the combined treatment (due to an increase in the number of -lymphocytes with cytotoxic-suppressive properties at obvious signs of immune disbalance in the form of the increased ratio of Т CD4+ and CD8+).

scholarly journals Method for the determination of immune complexes by reaction with polyethylene glycol

1982 ◽  
Vol 63 (2) ◽  
pp. 10-13
Author(s):  
B. A. Molotilov ◽  
A. N. Mayansky ◽  
N. D. Pozdnyak ◽  
L. Ch. Samerkhanova
Keyword(s):  
Polyethylene Glycol ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Clinical Laboratory ◽  
Circulating Immune Complexes ◽  
Healthy Donors ◽  
Highly Sensitive ◽  
Photoelectric Colorimeter ◽  
Health And Disease

A study of circulating immune complexes was carried out using a reaction with polyethylene glycol. The method turned out to be simple, highly sensitive and affordable for any clinical laboratory with a photoelectric colorimeter. Analysis of the survey data of 115 healthy donors, 63 patients with rheumatoid arthritis and 16 patients with systemic lupus erythematosus made it possible to establish the level of circulating immune complexes in health and disease. The circulating immune complexes were studied in patients with rheumatism and chronic tonsillitis. To assess the results of the reaction, human aggregated gamma globulin (manufactured by Kazan NIIEM) was used.

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