An experimental study has been performed for a buoyant jet diffusion flame, which was observed to oscillate at different frequencies spatially. The flame dynamics and structure were visualized by a commercial digital camera and a high-speed camera. Mixed fuel of methane and propane at certain proportion was found to generate very different vortex shedding behaviours. As a result, the flickering frequency of a methane/propane 1:1 mixture can be half of that of typically observed for pure methane or propane flame. The distance between adjacent flame puffs or the size of vortical structures in the reacting flow field, which can be modified by the fuel composition, was identified to be the key factor that affects the flickering frequencies. Repeated tests confirmed that mixed fuel at certain proportions can have a significant effect on the flame flickering frequency through the modification of vortex structure and dynamics.