Chemoattractant agents and nerve growth factor stimulate human spermatozoal reactive oxygen species generation

Group 2 allergen of Dermatophagoides pteronyssinus 2 (Der p2) induces airway inflammation without protease activity, and elevated nerve growth factor (NGF) levels are also found in this inflammation. How the allergen Der p2 regulates NGF release via reactive oxygen species (ROS) to induce inflammation remains unclear. In the present study, intratracheal administration of Der p2 to mice led to inflammatory cell infiltration, mucus gland hyperplasia, and NGF upregulation in the bronchial epithelium, as well as elevated ROS and NGF production in bronchoalveolar lavage fluids. In addition, Der p2 caused fibrocyte accumulation and mild fibrosis. p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) inhibitors inhibited Der p2-induced NGF release in LA4 lung epithelial cells and MLg lung fibroblasts. Pretreatment with an antioxidant, tiron, reduced the Der p2-induced ROS production, NGF expression and release, p38 MAPK or JNK phosphorylation, and airway inflammation. These results suggest that Der p2 allergen-induced airway inflammation and elevated NGF release were through increasing ROS production and a MAPK-dependent pathway. The use of an antioxidant, tiron, may provide a new therapeutic modality for the treatment of allergic asthma.

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Recruitment of Nox4 to a Plasma Membrane Scaffold Is Required for Localized Reactive Oxygen Species Generation and Sustained Src Activation in Response to Insulin-like Growth Factor-I

Journal of Biological Chemistry ◽

10.1074/jbc.m113.456046 ◽

2013 ◽

Vol 288 (22) ◽

pp. 15641-15653 ◽

Cited By ~ 31

Author(s):

Gang Xi ◽

Xin-Chun Shen ◽

Christine Wai ◽

David R. Clemmons

Keyword(s):

Reactive Oxygen Species ◽

Plasma Membrane ◽

Growth Factor ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Oxygen Species ◽

Insulin Like Growth Factor ◽

Factor I ◽

Growth Factor I ◽

Membrane Scaffold

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Faculty Opinions recommendation of Architecture of succinate dehydrogenase and reactive oxygen species generation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1012126.185766 ◽

2003 ◽

Author(s):

Tracy Palmer

Keyword(s):

Reactive Oxygen Species ◽

Succinate Dehydrogenase ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Oxygen Species

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Роль оксида азота в реализации антиоксидантного эффекта неопиатного аналога лей-энкефалина в сердце новорожденных белых крыс

Dalʹnevostočnyj medicinskij žurnal ◽

10.35177/1994-5191-2019-3-61-64 ◽

2019 ◽

pp. 61-64

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Postnatal Period ◽

Oxide System ◽

No Synthase ◽

Albino Rats ◽

Oxygen Species ◽

Control Parameters ◽

Synthase Inhibitor

The eff ect of the non-opiate analog of leu-enkephalin (peptide NALE: Phe – D – Ala – Gly – Phe – Leu – Arg) on the reactive oxygen species generation in the heart of albino rats in the early postnatal period was studied. Peptide NALE was administered intraperitoneally in the dose of 100 μ/kg daily from 2 to 6 days of life. Reactive oxygen species generation was assessed by chemiluminescence in the heart homogenates of 7-day-old animals. Decreasing of reactive oxygen species generation nearly by 30 % and an increasing in antioxidant system activity by the 20-27 %, compared with the control parameters, were found. The antioxidant eff ect of peptide NALE is associated with the presence of the amino acid Arg in the structure of the peptide. An analogue of NALE peptide, devoid of Arg (peptide Phe – D – Ala – Gly – Phe – Leu – Gly), had a signifi cant lower antioxidant eff ect. The NO-synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) in the dose 50 mg/kg, administered with NALE peptide, reduced the severity of the NALE antioxidant eff ect. The results of the study suggest that the pronounced antioxidant eff ect of NALE peptide in the heart of albino rats, at least in part, is due to the interaction with the nitric oxide system.

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