Human endometrial stromal cells improve embryo quality by enhancing the expression of insulin-like growth factors and their receptors in cocultured human preimplantation embryos

1999 ◽  
Vol 71 (2) ◽  
pp. 361-367 ◽  
Author(s):  
Hung-Ching Liu ◽  
Zhi-Ying He ◽  
Carol A Mele ◽  
Lucinda L Veeck ◽  
Owen Davis ◽  
...  
Keyword(s):  
Growth Factors ◽  
Stromal Cells ◽  
Embryo Quality ◽  
Preimplantation Embryos ◽  
Endometrial Stromal Cells ◽  
Human Preimplantation Embryos ◽  
Insulin Like Growth Factors

scholarly journals High-quality human preimplantation embryos stimulate endometrial stromal cell migration via secretion of microRNA hsa-miR-320a

2020 ◽  
Author(s):  
Robbert P. Berkhout ◽  
Remco Keijser ◽  
Sjoerd Repping ◽  
Cornelis B. Lambalk ◽  
Gijs B. Afink ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Micro Rna ◽  
Human Reproduction ◽  
Limiting Factors ◽  
Preimplantation Embryos ◽  
Success Rates ◽  
High Quality ◽  
Endometrial Stromal Cells ◽  
Altered Expression ◽  
Human Preimplantation Embryos

AbstractImplantation failure is one of the major success limiting factors in human reproduction. Despite, the mechanisms that determine successful human embryo implantation remain largely unknown. We here show that high-quality human preimplantation embryos secrete soluble signaling factors, including micro RNA (miRNA) hsa-miR-320a, that promote migration of human endometrial stromal cells (hESCs). By using miRNA mimics and inhibitors, we demonstrate that hsa-miR-320a alone can stimulate migration of decidualized hESCs, accurately resembling the response typically triggered only by high-quality embryos. Transcriptome analysis further demonstrated that this effect is very likely mediated via altered expression of genes involved in cell adhesion and cytoskeleton organization. In conclusion, by secreting hsa-miR-320a, high-quality human preimplantation embryos directly influence endometrial stromal cells, most likely to prime the endometrium at the implantation site for successful implantation. Together, our results indicate that hsa-miR-320a may be a promising target to further increase success rates in assisted reproduction.

scholarly journals Response of Bipotential Human Marrow Stromal Cells to Insulin-Like Growth Factors: Effect on Binding Protein Production, Proliferation, and Commitment to Osteoblasts and Adipocytes1

Endocrinology ◽  
1999 ◽  
Vol 140 (11) ◽  
pp. 5036-5044 ◽  
Author(s):  
Thierry Thomas ◽  
Francesca Gori ◽  
Thomas C. Spelsberg ◽  
Sundeep Khosla ◽  
B. Lawrence Riggs ◽  
...  
Keyword(s):  
Growth Factors ◽  
Stromal Cells ◽  
Protein Production ◽  
Binding Protein ◽  
Marrow Stromal Cells ◽  
Insulin Like Growth Factors
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