Pretein kinase C isoforms α, βII and δ are expressed in gallbladder epithelium during gallstone formation

2001 ◽  
Vol 120 (5) ◽  
pp. A386-A386 ◽  
Author(s):  
S NARINS ◽  
E PARK ◽  
X SU ◽  
P SMITH ◽  
M ABEDIN
Keyword(s):  
Gallstone Formation ◽  
Gallbladder Epithelium ◽  
Kinase C

Pretein kinase C isoforms α, βII and δ are expressed in gallbladder epithelium during gallstone formation

2001 ◽  
Vol 120 (5) ◽  
pp. A386
Author(s):  
Seth C. Narins ◽  
Eun H. Park ◽  
Xuefeng Su ◽  
Peter R. Smith ◽  
Mohammad Z. Abedin
Keyword(s):  
Gallstone Formation ◽  
Gallbladder Epithelium ◽  
Kinase C

scholarly journals Histopathological changes in gallbladder mucosa associated with cholelithiasis

1970 ◽  
Vol 2 (3) ◽  
pp. 224-225 ◽  
Author(s):  
R Baidya ◽  
B Sigdel ◽  
NL Baidya
Keyword(s):  
Retrospective Study ◽  
Intestinal Metaplasia ◽  
Gastrointestinal Disorder ◽  
Gallstone Formation ◽  
Epithelial Hyperplasia ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Gallbladder Epithelium ◽  
Gallbladder Mucosa ◽  
Gross Examination

Background: Gallstone is a very common gastrointestinal disorder. It is known to produce histopathological changes in the gallbladder. It is also one of the predisposing factors for the development of cancer of gallbladder. Materials and Methods: This was a retrospective study carried out in the Department of Histopathology, B and B Hospital during a period of 1 year from April 2010 to May 2011. The study included 396 cases of cholecystectomy specimens. Results: On gross examination, outer gallbladder surface was congested in 116 patients (28%), wall thickness was increased in 181 (45.7%) and mucosal abnormalities were present in 126 (31.6%) patients. At microscopy, epithelial hyperplasia was observed in 183 (46.2%), intestinal metaplasia in 112 (28.2%), dysplasia in 5 (1.3%) and cholesterolosis in 56 (14%) patients. Conclusion: The pathological changes of the gallbladder epithelium may play an important role in the process of gallstone formation. DOI: http://dx.doi.org/10.3126/jpn.v2i3.6027 JPN 2012; 2(3): 224-225

Protein kinase C-α regulation of gallbladder Na+ transport becomes progressively more dysfunctional during gallstone formation

2005 ◽  
Vol 146 (4) ◽  
pp. 227-237 ◽  
Author(s):  
Seth C. Narins ◽  
Ramugounder Ramakrishnan ◽  
Eun H. Park ◽  
Paul B. Bolno ◽  
David A. Haggerty ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Gallstone Formation ◽  
Na Transport ◽  
Kinase C

Enhanced fluid transport across gallbladder mucosa in experimental cholelithiasis.

1978 ◽  
Vol 234 (6) ◽  
pp. E575
Author(s):  
S P Lee
Keyword(s):  
Cell Proliferation ◽  
Guinea Pig ◽  
Fluid Transport ◽  
Stone Formation ◽  
Gallstone Formation ◽  
Gallbladder Epithelium ◽  
Histological Features ◽  
Gallbladder Mucosa ◽  
Fluid Transfer

Fluid transport of the gallbladder has been studied in two models of experimental cholelithiasis: dihydrocholesterol-induced gallstones in the rabbit and lincomycin-induced gallstones in the guinea pig. Using the noneverted explained gallbladder of the rabbit and the guinea pig, the transport of luminal to serosal fluid has been quantitated before, during, and after stone formation. The everted gallbladder preparation of the rabbit has also been used to measure fluid transport before and during gallstone formation. In both models, an increased fluid transport was observed in the phase of gallstone induction and a return to normal after stones were formed. This abnormality preceded the appearance of conventional histological features of cholecystitis. There was also a coincidental increase in glycoprotein production from and cell proliferation of the gallbladder epithelium. This enhancement of fluid transfer may play a contributing role in the genesis of gallstones.

An Immunohistochemical Study of Osteopontin in Pigment Gallstone Formation

2010 ◽  
Vol 76 (1) ◽  
pp. 91-95
Author(s):  
Motohiro Imano ◽  
Takao Satou ◽  
Tatsuki Itoh ◽  
Yoshifumi Takeyama ◽  
Atsushi Yasuda ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Matrix Protein ◽  
Bone Matrix ◽  
Gallstone Formation ◽  
Gallbladder Wall ◽  
Gallbladder Epithelium ◽  
Reticular Pattern ◽  
The Matrix ◽  
Pigment Gallstone

Mucin glycoproteins from the gallbladder epithelium are thought to contribute to the matrix or nucleus of gallstones and other biomineralization systems. The involved acidic glycoproteins have been reported in bile and gallstones. In addition, osteopontin (Opn) is a noncollagenous acidic bone matrix glycoprotein that possesses calcium-binding properties. To investigate the role of Opn in pigment gallstone formation, the involvement of Opn in pigment gallstone formation was studied immunohistochemically in the gallbladder wall and in the stones. Staining for Opn was strongly positive in the epithelium of stone-laden gallbladders and in their stones. The stone-laden gallbladders were infiltrated by macrophages, which intensely stained for Opn. Sections of the pigment stones, under low magnification, showed a lamellar pattern of Opn immunolabeling and showed a reticular pattern under high magnification. Our results indicate that Opn, an acidic glycoprotein from the gallbladder epithelium, seems to be involved in lithiasis. Opn from macrophages and/or the epithelium seems to help form the matrix protein.

Primary culture of human gallbladder epithelium: A new tool for studying gallstone formation

1998 ◽  
Vol 114 ◽  
pp. A533
Author(s):  
KE Morgenstern ◽  
GB Balsara ◽  
ZR Abedin ◽  
JJ Roslyn ◽  
MZ Abedin ◽  
...  
Keyword(s):  
Primary Culture ◽  
Gallstone Formation ◽  
Gallbladder Epithelium ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium

CFTR is expressed by gallbladder epithelium: A potential regulator of chloride and mucus secretion during gallstone formation

1998 ◽  
Vol 114 ◽  
pp. A532-A533 ◽  
Author(s):  
K.E. Morgenstern ◽  
Z.R. Abedin ◽  
P.R. Smith ◽  
A.J. Moser ◽  
M.Z. Abedin ◽  
...  
Keyword(s):  
Gallstone Formation ◽  
Mucus Secretion ◽  
Gallbladder Epithelium

Ultrastructural localization of secretory glycoconjugates in the gallbladder epithelium of the Richardson’s ground squirrel before and during cholesterol gallstone formation

1990 ◽  
Vol 48 (3) ◽  
pp. 926-927
Author(s):  
Brian R. MacPherson
Keyword(s):  
Ground Squirrel ◽  
Physiological Response ◽  
Potential Role ◽  
Cholesterol Gallstone ◽  
Ultrastructural Localization ◽  
Gallstone Formation ◽  
Ground Squirrels ◽  
Crystal Nucleation ◽  
Gallbladder Epithelium ◽  
Critical Feature

Hypersecretion of mucin is a critical feature of gallbladder epithelial cells in ground squirrels fed a 2% cholesterol-enriched diet for the induction of cholesterol gallstone formation. The timing of this physiological response indicates a potential role for mucin as an initiator of cholesterol crystal nucleation from supersaturated bile. There is little information regarding the specific type of glycoprotein secreted by gallbladder epithelia and routine light microscope histochemistry proved unproductive for detection of specific glycoprotein types being synthesized as the intracellular accumulations were not large enough to permit visualization of a colored precipitate. Ultrastructural histochemistry was utilized to differentiate between the two main mucin types secreted by the gastrointestinal tract - sulphated and sialomucins.Two ultrastructural methods utilizing the histochemical basis of the high and low iron diamine techniques were used. The high iron diamine (HID) technique imparts density to sulphated complex carbohydrates while the low iron diamine (LID) method imparts density to acidic glycoconjugates.

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