Antibiotic resistance to clarithromycin and point mutation in the 23S rRNA gene in Helicobacter pylori strains isolated from Korea

2001 ◽  
Vol 120 (5) ◽  
pp. A592-A592
Author(s):  
J KIM ◽  
Y YANG ◽  
K CHUNG ◽  
J PARK ◽  
B YOO
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Point Mutation ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

Antibiotic resistance to clarithromycin and point mutation in the 23S rRNA gene in Helicobacter pylori strains isolated from Korea

2001 ◽  
Vol 120 (5) ◽  
pp. A592
Author(s):  
Jae Gyu Kim ◽  
Yool Hee Yang ◽  
Kyu Chung ◽  
Jungwon Park ◽  
Byung Cheol Yoo
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Point Mutation ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

Low prevalence of clarithromycin-resistant Helicobacter pylori isolates with A2143G point mutation in the 23S rRNA gene in North India

2016 ◽  
Vol 6 ◽  
pp. 39-43 ◽  
Author(s):  
Valentina Gehlot ◽  
Shweta Mahant ◽  
Asish Kumar Mukhopadhyay ◽  
Kunal Das ◽  
Jawed Alam ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Point Mutation ◽  
North India ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene ◽  
Low Prevalence

scholarly journals High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population

2020 ◽  
Vol 8 (7) ◽  
pp. 1062
Author(s):  
Dashdorj Azzaya ◽  
Boldbaatar Gantuya ◽  
Khasag Oyuntsetseg ◽  
Duger Davaadorj ◽  
Takashi Matsumoto ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Gene Mutations ◽  
23S Rrna ◽  
Rrna Gene ◽  
Resistance Mutations ◽  
Standard Triple Therapy ◽  
23S Rrna Gene ◽  
H Pylori ◽  
Next Generation Sequencing Ngs

Mongolia has a high prevalence of Helicobacter pylori infection and the second highest incidence of gastric cancer worldwide. Thus, investigating the prevalence of antibiotic resistance and its underlying genetic mechanism is necessary. We isolated 361 H. pylori strains throughout Mongolia. Agar dilution assays were used to determine the minimum inhibitory concentrations of five antibiotics; amoxicillin, clarithromycin, metronidazole, levofloxacin, and minocycline. The genetic determinants of antibiotic resistance were identified with next-generation sequencing (NGS) and the CLC Genomics Workbench. The resistance to metronidazole, levofloxacin, clarithromycin, amoxicillin, and minocycline was 78.7%, 41.3%, 29.9%, 11.9% and 0.28%, respectively. Multidrug resistance was identified in 51.3% of the isolates investigated which were further delineated into 9 antimicrobial resistance profiles. A number of known antibiotic resistance mutations were identified including rdxA, frxA (missense, frameshift), gyrA (N87K, A88P, D91G/N/Y), 23S rRNA (A2143G), pbp1A (N562Y), and 16S rRNA (A928C). Furthermore, we detected previously unreported mutations in pbp1A (L610*) and the 23S rRNA gene (A1410G, C1707T, A2167G, C2248T, and C2922T). The degree of antibiotic resistance was high, indicating the insufficiency of standard triple therapy in Mongolia.

scholarly journals Distribution of Antibiotic MICs for Helicobacter pylori Strains over a 16-Year Period in Patients from Seoul, South Korea

2004 ◽  
Vol 48 (12) ◽  
pp. 4843-4847 ◽  
Author(s):  
Jung Mogg Kim ◽  
Joo Sung Kim ◽  
Hyun Chae Jung ◽  
Nayoung Kim ◽  
Young-Jeon Kim ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
South Korea ◽  
Clinical Problem ◽  
23S Rrna ◽  
Rrna Gene ◽  
Primary Resistance ◽  
The Past ◽  
23S Rrna Gene ◽  
H Pylori

ABSTRACT Recently, the development of antibiotic resistance emerged as a significant clinical problem in the eradication of Helicobacter pylori. We investigated the MICs of antibiotics for 135 H. pylori isolates from adults in Seoul, South Korea, over the past 16 years. The MICs of amoxicillin, clarithromycin, metronidazole, tetracycline, azithromycin, and ciprofloxacin increased from 1987 to 2003. Rates of primary resistance to clarithromycin increased from 2.8% in 1994 to 13.8% in 2003. The A2144G mutation was frequently observed in the 23S rRNA gene in clarithromycin-resistant isolates. The increase in resistance to clarithromycin seems to result in a decrease in eradication efficacy for H. pylori. These results suggest that the MICs of several antibiotics for H. pylori have increased over the past 16 years in Seoul.

Identification of a 23S rRNA Gene Mutation in Clarithromycin-Resistant Helicobacter pylori

Helicobacter ◽  
1996 ◽  
Vol 1 (4) ◽  
pp. 227-228 ◽  
Author(s):  
Gregory G. Stone ◽  
Dee Shortridge ◽  
Robert K. Flamm ◽  
James Versalovic ◽  
Jill Beyer ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gene Mutation ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene

scholarly journals Genotypic Characterization of Clarithromycin-Resistant and -Susceptible Helicobacter pylori Strains from the Same Patient Demonstrates Existence of Two Unrelated Isolates

1998 ◽  
Vol 36 (9) ◽  
pp. 2730-2731 ◽  
Author(s):  
Ge Wang ◽  
Qin Jiang ◽  
Diane E. Taylor
Keyword(s):  
Helicobacter Pylori ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Pulsed Field Gel Electrophoresis ◽  
23S Rrna ◽  
Rrna Gene ◽  
Clarithromycin Resistance ◽  
23S Rrna Gene ◽  
Mode Of Development

Clarithromycin-susceptible and clarithromycin-resistantHelicobacter pylori isolates from the same patient were investigated for the mode of development and mechanism of clarithromycin resistance. The clarithromycin-resistant strain UA1182 harbors homozygous A-to-G mutations at position 2143 in both copies of the 23S rRNA gene and has a phenotype of resistance to clarithromycin and clindamycin but no significant resistance to streptogramin B. Pulsed-field gel electrophoresis patterns of NruI- andNotI-digested genomic DNA from the Clas and Clar isolates demonstrated that they are genetically distinct, suggesting that the development of clarithromycin resistance is not from the mutation of the existing Clas strain but from a completely new strain.

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