M1954 A Telomerase-Immortalized, Non-Neoplastic, Human Barrett's Cell Line As Model of Ion Transport and Barrier Function of Native Barrett's Specialized Columnar Epithelium

2008 ◽  
Vol 134 (4) ◽  
pp. A-438
Author(s):  
Biljana Jovov ◽  
Geraldine S. Orlando ◽  
Karen L. Brown ◽  
Roy C. Orlando
Keyword(s):  
Ion Transport ◽  
Cell Line ◽  
Barrier Function ◽  
Columnar Epithelium ◽  
Specialized Columnar Epithelium

Ion transport and barrier function in a telomerase-immortalized human nondysplastic, Barrett's cell line (BAR-T)

2009 ◽  
Vol 22 (5) ◽  
pp. 386-395 ◽  
Author(s):  
Biljana Jovov ◽  
Geraldine S. Orlando ◽  
Nelia A. Tobey ◽  
Karen L. Brown ◽  
Zorka Djukic ◽  
...  
Keyword(s):  
Ion Transport ◽  
Cell Line ◽  
Barrier Function

NCL-SG3: a human eccrine sweat gland cell line that retains the capacity for transepithelial ion transport

1989 ◽  
Vol 92 (2) ◽  
pp. 241-249
Author(s):  
C.M. Lee ◽  
J. Dessi
Keyword(s):  
Epithelial Cell ◽  
Ion Transport ◽  
Cell Line ◽  
Intermediate Filament ◽  
Primary Cultures ◽  
Simian Virus 40 ◽  
T Antigen ◽  
Sweat Glands ◽  
Epithelial Cell Line ◽  
Eccrine Sweat

An ion-transporting human epithelial cell line, NCL-SG3, has been established by simian virus 40 (SV40) infection of primary cultures from eccrine sweat glands. The line has been passaged 38 times (over 100 population doublings), has an aneuploid karyotype but has not undergone any ‘crisis’. The cells have retained epithelial morphology and expression of cytokeratin, the intermediate filament characteristic of epithelial cells. Approximately 85% of the population shows at least weak co-expression of vimentin, an intermediate filament associated with mesenchymal and some other non-epithelial cell types in vivo. In addition, SV40 large T-antigen is present, in a predominantly nuclear localization. Electrically resistant cell sheets are formed on dialysis tubing and cellulose-ester permeable supports. Electrogenic ion transport can be stimulated by the beta-adrenergic agonist isoproterenol (10(−6) M) and by lysylbradykinin (10(−7) M) but not by the cholinergic agonist carbachol at 10(−6) M).

scholarly journals Characterization of the ion transport responses to ADH in the MDCK-C7 cell line

2000 ◽  
Vol 439 (5) ◽  
pp. 610-617 ◽  
Author(s):  
T.F. Lahr ◽  
R.D. Record ◽  
D.K. Hoover ◽  
C.L. Hughes ◽  
B.L. Blazer-Yost
Keyword(s):  
Ion Transport ◽  
Cell Line

Extracellular ATP Modulates Ion Transport via P2Y Purinoceptors in a Middle-Ear Epithelial Cell Line

ORL ◽  
1997 ◽  
Vol 59 (3) ◽  
pp. 170-175 ◽  
Author(s):  
P.-T. Yen ◽  
P. Herman ◽  
T. van den Abbeele ◽  
C.-T. Tan ◽  
P. Bordure ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Ion Transport ◽  
Cell Line ◽  
Middle Ear ◽  
Extracellular Atp ◽  
Epithelial Cell Line

Vasoactive intestinal peptide stimulates protein phosphorylation in a colonic epithelial cell line

1987 ◽  
Vol 253 (3) ◽  
pp. G420-G424 ◽  
Author(s):  
J. A. Cohn
Keyword(s):  
Epithelial Cell ◽  
Ion Transport ◽  
Protein Phosphorylation ◽  
Cell Line ◽  
Vasoactive Intestinal Peptide ◽  
Epithelial Cell Line ◽  
Colonic Epithelial Cell ◽  
Ussing Chambers ◽  
Colonic Epithelial Cell Line ◽  
Stimulation Of

The T84 colonic epithelial cell line was used to examine protein phosphorylation during neurohumoral stimulation of ion transport. T84 cell monolayers grown on collagen-coated filters were mounted in Ussing chambers to measure ion transport stimulated by vasoactive intestinal peptide. Maximal stimulation of active secretion occurred after 8-10 min of stimulation. Protein phosphorylation events accompanying stimulated secretion were detected using two-dimensional gel electrophoresis to resolve phosphoproteins from monolayers previously labeled using 32Pi. Within 8 min of exposure to vasoactive intestinal peptide, several phosphorylation events were detected, including a two- to fivefold increase in 32P incorporation into four soluble proteins with apparent molecular weights of 17,000, 18,000, 23,000, and 37,000. The same phosphorylation response occurs in monolayers stimulated by dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP), suggesting that cAMP mediates these intracellular events. This study indicates that changes in protein phosphorylation accompany the secretory action of vasoactive intestinal peptide and suggests that T84 cells offer a useful model for studying the possibility that such phosphorylation events regulate enterocyte ion transport.

Barrier function of microvessels and roles of glial cell line-derived neurotrophic factor in the rat testis

2002 ◽  
Vol 35 (3) ◽  
pp. 139-145 ◽  
Author(s):  
Y. Kamimura ◽  
Hideki Chiba ◽  
H. Utsumi ◽  
Tomoko Gotoh ◽  
Hirotoshi Tobioka ◽  
...  
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Barrier Function ◽  
Rat Testis

PAR-2-mediated control of barrier function and motility differs between early and late phases of postinfectious gut dysfunction in the rat

2013 ◽  
Vol 304 (4) ◽  
pp. G390-G400 ◽  
Author(s):  
Juan A. Fernández-Blanco ◽  
Morley D. Hollenberg ◽  
Vicente Martínez ◽  
Patri Vergara
Keyword(s):  
Ion Transport ◽  
Rat Model ◽  
Barrier Function ◽  
Early Phase ◽  
Trichinella Spiralis ◽  
Late Phase ◽  
Organ Bath ◽  
Motor Responses ◽  
Gut Dysfunction

Proteinase-activated receptor-2 (PAR-2) and mast cell (MC) mediators contribute to inflammatory and functional gastrointestinal disorders. We aimed to characterize jejunal PAR-2-mediated responses and the potential MC involvement in the early and late phases of a rat model of postinfectious gut dysfunction. Jejunal tissues of control and Trichinella spiralis-infected (14 and 30 days postinfection) rats, treated or not with the MC stabilizer, ketotifen, were used. Histopathology and immunostaining were used to characterize inflammation, PAR-2 expression, and mucosal and connective tissue MCs. Epithelial barrier function (hydroelectrolytic transport and permeability) and motility were assessed in vitro in basal conditions and after PAR-2 activation. Intestinal inflammation on day 14 postinfection (early phase) was significantly resolved by day 30 (late phase) although MC counts and epithelial permeability remained increased. PAR-2-mediated ion transport (Ussing chambers, in vitro) and epithelial surface PAR-2 expression were reduced in the early phase, with a trend toward normalization during the late phase. In control conditions, PAR-2 activation (organ bath) induced biphasic motor responses (relaxation followed by excitation). At 14 days postinfection, spontaneous contractility and PAR-2-mediated relaxations were enhanced; motor responses were normalized on day 30. Postinfectious changes in PAR-2 functions were not affected by ketotifen treatment. We concluded that, in the rat model of Trichinella spiralis infection, alterations of intestinal PAR-2 function and expression depend on the inflammatory phase considered. A lack of a ketotifen effect suggests no interplay between MCs and PAR-2-mediated motility and ion transport alterations. These observations question the role of MC mediators in PAR-2-modulating postinfectious gut dysfunction.

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