NCL-SG3: a human eccrine sweat gland cell line that retains the capacity for transepithelial ion transport

1989 ◽  
Vol 92 (2) ◽  
pp. 241-249
Author(s):  
C.M. Lee ◽  
J. Dessi
Keyword(s):  
Epithelial Cell ◽  
Ion Transport ◽  
Cell Line ◽  
Intermediate Filament ◽  
Primary Cultures ◽  
Simian Virus 40 ◽  
T Antigen ◽  
Sweat Glands ◽  
Epithelial Cell Line ◽  
Eccrine Sweat

An ion-transporting human epithelial cell line, NCL-SG3, has been established by simian virus 40 (SV40) infection of primary cultures from eccrine sweat glands. The line has been passaged 38 times (over 100 population doublings), has an aneuploid karyotype but has not undergone any ‘crisis’. The cells have retained epithelial morphology and expression of cytokeratin, the intermediate filament characteristic of epithelial cells. Approximately 85% of the population shows at least weak co-expression of vimentin, an intermediate filament associated with mesenchymal and some other non-epithelial cell types in vivo. In addition, SV40 large T-antigen is present, in a predominantly nuclear localization. Electrically resistant cell sheets are formed on dialysis tubing and cellulose-ester permeable supports. Electrogenic ion transport can be stimulated by the beta-adrenergic agonist isoproterenol (10(−6) M) and by lysylbradykinin (10(−7) M) but not by the cholinergic agonist carbachol at 10(−6) M).

scholarly journals Characterization of doxycycline-dependent inducible Simian Virus 40 large T antigen immortalized human conjunctival epithelial cell line

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222454
Author(s):  
Arisa Mitani ◽  
Takeshi Kobayashi ◽  
Yasuhito Hayashi ◽  
Natsuki Matsushita ◽  
Sachi Matsushita ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Epithelial Cell Line ◽  
Large T Antigen ◽  
Conjunctival Epithelial Cell

Extracellular ATP Modulates Ion Transport via P2Y Purinoceptors in a Middle-Ear Epithelial Cell Line

ORL ◽  
1997 ◽  
Vol 59 (3) ◽  
pp. 170-175 ◽  
Author(s):  
P.-T. Yen ◽  
P. Herman ◽  
T. van den Abbeele ◽  
C.-T. Tan ◽  
P. Bordure ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Ion Transport ◽  
Cell Line ◽  
Middle Ear ◽  
Extracellular Atp ◽  
Epithelial Cell Line

Vasoactive intestinal peptide stimulates protein phosphorylation in a colonic epithelial cell line

1987 ◽  
Vol 253 (3) ◽  
pp. G420-G424 ◽  
Author(s):  
J. A. Cohn
Keyword(s):  
Epithelial Cell ◽  
Ion Transport ◽  
Protein Phosphorylation ◽  
Cell Line ◽  
Vasoactive Intestinal Peptide ◽  
Epithelial Cell Line ◽  
Colonic Epithelial Cell ◽  
Ussing Chambers ◽  
Colonic Epithelial Cell Line ◽  
Stimulation Of

The T84 colonic epithelial cell line was used to examine protein phosphorylation during neurohumoral stimulation of ion transport. T84 cell monolayers grown on collagen-coated filters were mounted in Ussing chambers to measure ion transport stimulated by vasoactive intestinal peptide. Maximal stimulation of active secretion occurred after 8-10 min of stimulation. Protein phosphorylation events accompanying stimulated secretion were detected using two-dimensional gel electrophoresis to resolve phosphoproteins from monolayers previously labeled using 32Pi. Within 8 min of exposure to vasoactive intestinal peptide, several phosphorylation events were detected, including a two- to fivefold increase in 32P incorporation into four soluble proteins with apparent molecular weights of 17,000, 18,000, 23,000, and 37,000. The same phosphorylation response occurs in monolayers stimulated by dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP), suggesting that cAMP mediates these intracellular events. This study indicates that changes in protein phosphorylation accompany the secretory action of vasoactive intestinal peptide and suggests that T84 cells offer a useful model for studying the possibility that such phosphorylation events regulate enterocyte ion transport.

Immortalization of Rat Eustachian Tube Epithelial Cells by Adenovirus 12-Simian Virus 40 Hybrid Virus

2002 ◽  
Vol 111 (10) ◽  
pp. 919-925 ◽  
Author(s):  
Sunji Jin ◽  
Shigehiro Ueyama ◽  
Sung-Kyun Moon ◽  
Johng S. Rhim ◽  
Xin-Xing Gu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Line ◽  
Otitis Media ◽  
Eustachian Tube ◽  
Primary Cultures ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Male Rat ◽  
Positive Staining

The eustachian tube epithelial cells play an important role in the initial pathogenesis of otitis media. In order to study the role of the eustachian tube epithelial cells in the pathogenesis of otitis media, we have established a rat eustachian tube epithelial cell line. The cell line was derived by infecting primary cultures of eustachian tube epithelial cells with the adenovirus 12-simian virus 40 (Ad12-SV40) hybrid virus. The immortalized cells have retained the morphological characteristics of the parental cells and show positive staining with anti-cytokeratin antibodies (a marker for epithelial cells), but not with anti-vimentin antibodies (a fibroblast marker). The cells have been in continuous culture for more than 10 months and have undergone 38 passages. Western blotting and cell staining have confirmed the expression of the SV40 T antigen and p53. Chromosomal analysis indicates that the cell line is aneuploid and derived from male rat epithelial cells. Together, our results suggest that the cell line originated from eustachian tube epithelial cells from a male rat and was successfully immortalized by the Ad12-SV40 virus.

Establishment and characterization of a SV40 T-antigen immortalized human bronchial epithelial cell line

1992 ◽  
Vol 28 (7-8) ◽  
pp. 461-464 ◽  
Author(s):  
Joan H. Schiller ◽  
Chinghai Kao ◽  
Gerard Bittner ◽  
Chuck Harris ◽  
Terry D. Oberley ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Bronchial Epithelial Cell ◽  
T Antigen ◽  
Human Bronchial Epithelial Cell ◽  
Epithelial Cell Line ◽  
Sv40 T Antigen ◽  
Bronchial Epithelial
Sign in / Sign up

Export Citation Format

Share Document