scholarly journals Inhibitory effect of regucalcin on Ca2+ - dependent protein kinase activity in rat brain cytosol: Involvement of endogenous regucalcin

2000 ◽  
Vol 82 ◽  
pp. 71
Author(s):  
Yasuko Hanahisa ◽  
Toshie Hamano ◽  
Masayoshi Yamaguchi
Keyword(s):  
Protein Kinase ◽  
Rat Brain ◽  
Kinase Activity ◽  
Inhibitory Effect ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Brain Cytosol

Ca2+ and lipolysis in adipocytes from exercise-trained rats

1994 ◽  
Vol 77 (6) ◽  
pp. 2618-2624 ◽  
Author(s):  
T. Izawa ◽  
T. Komabayashi
Keyword(s):  
Protein Kinase ◽  
Adrenocorticotropic Hormone ◽  
Kinase Activity ◽  
Inhibitory Effect ◽  
Protein Kinase Activity ◽  
Dibutyryl Camp ◽  
Inhibitory Effects ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein

The effects of Ca2+ on lipolysis and protein kinase activity in adipocytes from exercise-trained rats were investigated. Chronic exercise significantly increased lipolytic responses to norepinephrine and dibutyryl adenosine 3′,5′-cyclic monophosphate (cAMP). The inhibitory effects of N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide (W-7), a calumodulin inhibitor, on norepinephrine- and dibutyryl cAMP-stimulated lipolysis were significantly greater in trained than in sedentary rats. Training did not alter cAMP-dependent protein kinase activity. However, the inhibitory effect of W-7 on cAMP-dependent protein kinase activity was much greater in trained than in sedentary rats. The basal intracellular free Ca2+ concentration ([Ca2+]i) was significantly higher in trained than in sedentary rats. The rapid and transient increases in [Ca2+]i due to adrenocorticotropic hormone and phenylephrine from basal levels were significantly lower in trained than in sedentary rats. However, the higher basal [Ca2+]i level in trained rats led to increases in sustained [Ca2+]i levels after stimulation. We concluded that in trained rats the regulation of protein kinase activity by cAMP depends to a greater degree on Ca(2+)-calmodulin complex than it does in sedentary rats and that training alters adipocyte intracellular Ca2+ homeostasis, including [Ca2+]i responsiveness to hormones.

Interaction of acetylcholine and epinephrine on heart cyclic AMP-dependent protein kinase

1978 ◽  
Vol 234 (4) ◽  
pp. H432-H438
Author(s):  
S. L. Keely ◽  
T. M. Lincoln ◽  
J. D. Corbin
Keyword(s):  
Protein Kinase ◽  
Kinase Activity ◽  
Inhibitory Effect ◽  
Contractile Force ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Kinase Activation ◽  
Camp Dependent Protein Kinase ◽  
Cholinergic Agent ◽  
Dependent Protein

In the isolated perfused rat heart, epinephrine produced a rapid, concentration-dependent increase in cyclic adenosine 3',5'-monophosphate (cAMP), activation of cAMP-dependent protein kinase, activation of phosphorylase, and increase in contractile force. At epinephrine concentrations of 1 micron or less, acetylcholine antagonized all these beta-adrenergic effects and also increased cyclic guanosine 3',5'-monophosphate (cGMP) levels. When used alone, acetylcholine produced a rapid elevation of cGMP and markedly diminished contractile force but did not significantly lower basal cAMP levels or cAMP-dependent protein kinase activity. The data suggest that changes in cAMP-dependent protein kinase activity can explain the antagonism of epinephrine-induced activation of phosphorylase by acetylcholine, but cannot completely account for the inhibitory effect of the cholinergic agent on contractile force.

Calcium/Ganglioside-Dependent Protein Kinase Activity in Rat Brain Membrane

1985 ◽  
Vol 44 (4) ◽  
pp. 1229-1234 ◽  
Author(s):  
James R. Goldenring ◽  
Laura C. Otis ◽  
Robert K. Yu ◽  
Robert J. DeLorenzo
Keyword(s):  
Protein Kinase ◽  
Rat Brain ◽  
Kinase Activity ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Brain Membrane ◽  
Dependent Protein
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