scholarly journals Effects of geraniin on the morphology and phagocytic activity of mouse peritoneal macrophages

1989 ◽  
Vol 49 ◽  
pp. 265
Author(s):  
Tang Fang ◽  
Yumiko Ushio ◽  
Hiroyoshi Konishi ◽  
Takuo Okuda ◽  
Hiroko Abe
Keyword(s):  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages

scholarly journals Interferon suppresses pinocytosis but stimulates phagocytosis in mouse peritoneal macrophages: related changes in cytoskeletal organization.

1984 ◽  
Vol 98 (4) ◽  
pp. 1328-1341 ◽  
Author(s):  
E Wang ◽  
J Michl ◽  
L M Pfeffer ◽  
S C Silverstein ◽  
I Tamm
Keyword(s):  
Horseradish Peroxidase ◽  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Cytochalasin D ◽  
Interferon Treatment ◽  
Cytoskeletal Organization ◽  
Sheep Erythrocytes ◽  
Beta Interferon ◽  
10 Nm Filaments ◽  
Mouse Peritoneal Macrophages

Treatment of thioglycolate-elicited macrophages with mouse beta-interferon markedly reduces pinocytosis of horseradish peroxidase and fluorescein isothiocyanate (FITC)-dextran but stimulates phagocytosis of IgG-coated sheep erythrocytes. Experiments with FITC-dextran have revealed that the overall decrease in pinocytosis is due to a nearly complete inhibition of pinocytosis in a large fraction of interferon-treated macrophages. In the remaining cells pinocytosis continues at a rate similar to that in untreated control cells. A considerable reduction in the number of cells pinocytosing FITC-dextran was observed within 12 h from the beginning of interferon treatment. Measurement of the overall level of pinocytic activity with horseradish peroxidase showed a progressive decline through 72 h of treatment. In the interferon-sensitive subpopulation, there were marked changes in cytoskeletal organization. Microtubules and 10-nm filaments were aggregated in the perinuclear region while most of the peripheral cytoplasm became devoid of these cytoskeletal structures as observed by fluorescence and electron microscopy. In addition, interferon treatment of macrophages appeared to disrupt the close topological association between bundles of 10-nm filaments and organelles such as mitochondria, lysosomes, and elements of the Golgi apparatus and endoplasmic reticulum. Such alterations in the distribution of microtubules and 10-nm filaments were not seen in the interferon-insensitive subpopulation. We have investigated the mechanism of the interferon-induced enhancement of phagocytic activity by binding IgG-coated sheep erythrocytes to mouse peritoneal macrophages at 4 degrees C and then initiating a synchronous round of ingestion by warming the cells to 37 degrees C. Thioglycolate-elicited macrophages that had been treated with mouse beta-interferon ingested IgG-coated erythrocytes faster and to a higher level than control cells in a single round of phagocytosis. In interferon-treated cultures, phagocytic cups became evident within 30 s of the shift of cultures from 4 degrees to 37 degrees C, whereas in control cultures, they appeared in 2 min. Cytochalasin D, an inhibitor of actin assembly and polymerization, abolished phagocytic activity in both control and beta-interferon-treated macrophages. However, to inhibit phagocytosis completely in thioglycolate-elicited interferon-treated macrophages, twice as much cytochalasin D was required in the treated as in control cultures.(ABSTRACT TRUNCATED AT 400 WORDS)

The effect of bilirubin on the fc receptor expression and phagocytic activity of mouse peritoneal macrophages

1985 ◽  
Vol 30 (4) ◽  
pp. 373-380 ◽  
Author(s):  
V. Větvička ◽  
I. Miler ◽  
P. Šíma ◽  
L. Táborský ◽  
L. Fornůsbk
Keyword(s):  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Fc Receptor ◽  
Receptor Expression ◽  
Mouse Peritoneal Macrophages

scholarly journals Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity

2021 ◽  
Vol 19 (1) ◽  
pp. 338-346
Author(s):  
Ying Wang ◽  
Yin-He Yang ◽  
Qing Luo ◽  
Yuan Hu ◽  
Qian Lu ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
High Performance ◽  
Synergistic Effects ◽  
Folk Medicine ◽  
Total Content ◽  
Monosaccharide Composition ◽  
Peritoneal Macrophages ◽  
Composition Analysis ◽  
Phagocytic Ability ◽  
Mouse Peritoneal Macrophages

Abstract Blaps rynchopetera Fairmaire has a long history of use as a folk medicine in China for treating fever, cough, gastritis, boils, and tumors. In the present study, the content analyses, monosaccharide composition analyses, and the macrophage phagocytic activity of rynchopetera polysaccharides (RPS) were reported. B. rhynchoptera is rich in polysaccharides (content value 3.97%). Through PMP (1-phenyl-3-methyl-5-pyrazolone) pre-column derivatization and high performance liquid chromatography (HPLC) testing, the results showed that RPS consist of 8 known monosaccharides, including D-mannose (Man), Rhamnose (Rha), D-glucuronic acid (GlcUA), D-galacturonic acid (GalUA), D-glucose (Glc), D-galactose (Gal), Arabinose (Ara), and Fucose (Fuc), with the total content of 171.70 mg g−1 and Glc has the highest content of 45.40 mg g−1. The phagocytic ability of mouse peritoneal macrophages was investigated after RPS stimulating alone and combined with lipopolysaccharide (LPS). RPS played an important role in the engulfment of mouse peritoneal macrophages and can significantly enhance the phagocytic ability of macrophages. However, no synergistic effects were observed when RPS combined with LPS.

3. Morphological parameters of the effect of interferon on mouse peritoneal macrophages

1982 ◽  
Vol 299 (1094) ◽  
pp. 131-133 ◽  
Keyword(s):  
Light Microscopy ◽  
Phagocytic Activity ◽  
Quantitative Data ◽  
Phase Contrast ◽  
Peritoneal Macrophages ◽  
Mouse Fibroblast ◽  
Morphological Parameters ◽  
Glass Surfaces ◽  
Mouse Peritoneal Macrophages

Several macrophage functions are modulated by treatment with homologous interferon (IFN). For example, phagocytic activity of mouse peritoneal macrophages (MPM) is enhanced by moderate concentrations of mouse fibroblast interferon (MuIFN-P) (Rollag & Degré 1981). Spreading of freshly seeded macrophages on glass surfaces is stimulated by macrophage-activating agents (Mörland & Kaplan 1977), IFN inducers (Rabinovitch et al . 1977) and IFN (Schultz et al . 1978). We report here quantitative data on effect of MuIFN-β on the spreading of MPM in vitro . Cells were seeded on glass surfaces in Eagle’s MEM, and spreading was examined after incubation at 37 °C for various periods by phase-contrast light microscopy (p.c.m.). Cells, fixed in 2.5% glutaraldehyde, were scored as round or spread, at least 200 cells in each preparation (Rabinovitch & De Stefano 1973).

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