glass surfaces
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Author(s):  
Jianxiong Hao ◽  
Junyi Zhang ◽  
Xueqi Zheng ◽  
Dandan Zhao

Abstract In the present study, the bactericidal efficacy of slightly acidic electrolyzed water (SAEW) against L. monocytogenes planktonic cells and biofilm on food-contact surfaces including stainless steel and glass was systematically evaluated. The results showed that SAEW (pH of 5.09 and available chlorine concentration (ACC) of 60.33 mg/L) could kill L. monocytogenes on food-contact surfaces completely in 30 s, whose disinfection efficacy is equal to that of NaClO solutions (pH of 9.23 and ACC of 253.53 mg/L). The results showed that long exposure time and high ACC contributed to the enhancement of the disinfection efficacy of SAEW on L. monocytogenes on food-contact surfaces. Moreover, the log reduction of SAEW treatment presented an increasing tendency within the prolonging of treatment time when SAEW was used to remove the L. monocytogenes biofilm formed on stainless steel and glass surfaces, which suggested that SAEW could remove L. monocytogenes biofilm effectively and its disinfection efficacy is equal to (in case of stainless steel) or higher than (in case of glass) that of high ACC of NaClO solutions. In addition, the results of the crystal violet staining and scanning electron microscopy (SEM) also demonstrated that SAEW treatment could remove the L. monocytogenes biofilm on food-contact surfaces.


2022 ◽  
Author(s):  
Vasco Rodrigues ◽  
Sarah Taheraly ◽  
Mathieu Maurin ◽  
Mabel San-Roman ◽  
Emma Granier ◽  
...  

A defining feature of HIV-1 replication in macrophages is that viral assembly occurs at the limiting membrane of a compartment often named VCC (virus-containing compartments) that is connected to the extracellular medium. The newly formed viral progeny pinches of the membrane and accumulates in the lumen of the VCC. While HIV budding has been extensively studied, very little is known about how viral particles present in the lumen of VCC are released in the extracellular medium. Here we show that the actin dynamics are critical for this process by combining ultrastructural analyses, time-lapse microscopy and perturbations of the actin cytoskeleton. We found that jasplakinolide, which stabilizes actin fibres, inhibited viral release from HIV-1-infected macrophages, but not from infected HeLa cells. Furthermore, in jasplakinolide-treated macrophages, VCC became scattered and no longer co-localized with the integrin CD18, nor the phosphorylated form of the focal adhesion kinase PYK2. Inhibition of PYK2 activity in infected macrophages promoted intracellular retention of viral particles in VCC that were no longer connected to the plasma membrane. Finally, we stimulated the rapid release of viral particles from the VCC by subjecting infected macrophages to frustrated phagocytosis. As macrophages spread on IgG-coated glass surfaces, VCC rapidly migrated to the basal membrane and released their viral content in the extracellular medium, which required their association with CD18 and the actin cytoskeleton. These results highlight that VCC trafficking and virus release are intimately linked to the reorganization of the macrophage actin cytoskeleton in response to external physical cues, suggesting that it might be regulated in tissues by the mechanical stress to which these cells are exposed.


2021 ◽  
Author(s):  
Isadonna Tengganu ◽  
Neil Karerakattil ◽  
Swarup Dey ◽  
Devika Kishnan ◽  
Rizal Hariadi

In vitro gliding assay is a well-established assay for determining the activity of protein motors, such as actin-associated myosins and microtubule-associated kinesins and dyneins. In one of the conventional methods, protein motors are immobilized onto a nitrocellulose-coated coverslip and it propels actin filaments in the presence of ATP. Gliding assays also serve as the foundation for protein-motor-based nanotechnological devices such as biosensing and sorting. However, the preparation of nitrocellulose-coated coverslips is time-consuming and produces rough surfaces. Furthermore, the nitrocellulose film exhibits high background autofluorescence, which can be a problem in single-molecule measurements. Here, we investigated the use of hexamethyldisilazane (HMDS) to study actomyosin function and characterized its physical properties on glass coverslips and glass capillary tubes. We showed that the total preparation time to coat a coverslip with HMDS is <30 minutes, which is 1 order of magnitude faster than the >12-hour protocol for coating glass surfaces with nitrocellulose. In contrast to nitrocellulose film, HMDS vapor deposition is effortless and provides an atomically flat surface with low autofluorescence. In addition, HMDS does not interfere with myosin function, which is indicated by the similar actin gliding speed when compared with nitrocellulose. Our results show that HMDS vapor deposition is a more favorable surface treatment to nitrocellulose for in vitro gliding assay.


2021 ◽  
Vol 5 (4) ◽  
pp. 34-41
Author(s):  
Sergei Poroshin ◽  
Victoriya Usik ◽  
Ihor Bielikov

The subject of research in the article is the methodology for the examination of small-volume premises with a predominance of speech content. The aim of the work is to analyze all stages of acoustic expertise for meeting rooms, conference rooms, press centers, to determine the volume and sequence of the stages, taking into account the specific restrictions and conditions that arise in small rooms. All stages of acoustic expertise are considered in the work on examples of real premises of meeting rooms, conference halls, press centers. The sequence of stages of the examination, when it was carried out for premises of small volumes, did not undergo any changes, compared to the sequence that is used for spectator halls. The main distinctive feature of the first stage of acoustic examination in small rooms with a predominance of speech content is the analysis of the structures of the reverberation process in listening places in order to identify the drawbacks of the formation of a diffuse field, instead of checking the geometry of the wall and ceiling panel walls using geometric theory. A feature of the second stage is the development of recommendations for improving the sound-absorbing properties of enclosing surfaces and eliminating the effect of multiple re-reflections of sound energy between parallel surfaces through the use of partial replacement of surface geometry, work with suspended ceiling structures and the use of sound-absorbing curtains (to correct the properties of glass surfaces). The third and fourth stages of the examination remained unchanged.


Author(s):  
Moshe Shirav ◽  
Gustavo Haquin

In most epidemiological studies, contemporary radon measurements are used as a proxy for radon concentrations during the latency period even though extreme changes in radon levels may have occurred. Airborne radon decay products are deposited and implanted through alpha recoil into exposed glass surfaces, providing a measure of time-integrated retrospective radon concentration in the environment in which the glass has been located.


Author(s):  
Nguyễn Hoàng Ly ◽  
Phuong‐Dong Nguyen ◽  
Sang Jun Son ◽  
Cheolmin Lee ◽  
Soonmin Jang ◽  
...  

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