scholarly journals The calmodulin-binding domain mediates the self-association of the plasma membrane Ca2+ pump.

1991 ◽  
Vol 266 (1) ◽  
pp. 22-27
Author(s):  
T Vorherr ◽  
T Kessler ◽  
F Hofmann ◽  
E Carafoli
Keyword(s):  
Plasma Membrane ◽  
Binding Domain ◽  
The Self ◽  
Calmodulin Binding ◽  
Self Association

scholarly journals The Calmodulin Binding Domain of the Plasma Membrane Ca2+ Pump Interacts Both with Calmodulin and with Another Part of the Pump

1989 ◽  
Vol 264 (21) ◽  
pp. 12313-12321 ◽  
Author(s):  
A Enyedi ◽  
T Vorherr ◽  
P James ◽  
D J McCormick ◽  
A G Filoteo ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Binding Domain ◽  
Calmodulin Binding

Interaction of calmodulin with the calmodulin binding domain of the plasma membrane calcium pump

Biochemistry ◽  
1990 ◽  
Vol 29 (2) ◽  
pp. 355-365 ◽  
Author(s):  
Thomas Vorherr ◽  
Peter James ◽  
Joachim Krebs ◽  
Agnes Enyedi ◽  
Daniel J. McCormick ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Binding Domain ◽  
Calcium Pump ◽  
Calmodulin Binding ◽  
Plasma Membrane Calcium Pump

scholarly journals Protein Kinase C Phosphorylates Plasma Membrane Ca2+Pump Isoform 4a at Its Calmodulin Binding Domain

1999 ◽  
Vol 274 (1) ◽  
pp. 527-531 ◽  
Author(s):  
Anil K. Verma ◽  
Katalin Paszty ◽  
Adelaida G. Filoteo ◽  
John T. Penniston ◽  
Agnes Enyedi
Keyword(s):  
Plasma Membrane ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Binding Domain ◽  
Calmodulin Binding ◽  
Kinase C

scholarly journals Plasma Membrane Calcium Pump Isoform 4a Has a Longer Calmodulin-Binding Domain Than 4b

1996 ◽  
Vol 271 (7) ◽  
pp. 3714-3718 ◽  
Author(s):  
Anil K. Verma ◽  
Agnes Enyedi ◽  
Adelaida G. Filoteo ◽  
Emanuel E. Strehler ◽  
John T. Penniston
Keyword(s):  
Plasma Membrane ◽  
Binding Domain ◽  
Calcium Pump ◽  
Calmodulin Binding ◽  
Plasma Membrane Calcium Pump

scholarly journals At-ACA8 Encodes a Plasma Membrane-Localized Calcium-ATPase of Arabidopsis with a Calmodulin-Binding Domain at the N Terminus

2000 ◽  
Vol 123 (4) ◽  
pp. 1495-1506 ◽  
Author(s):  
Maria Cristina Bonza ◽  
Piero Morandini ◽  
Laura Luoni ◽  
Markus Geisler ◽  
Michael Gjedde Palmgren ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Calcium Atpase ◽  
Binding Domain ◽  
Calmodulin Binding ◽  
N Terminus

scholarly journals Protein 4.1R self-association: identification of the binding domain

2006 ◽  
Vol 400 (3) ◽  
pp. 457-465
Author(s):  
Carmen M. Pérez-Ferreiro ◽  
Eva Lospitao ◽  
Isabel Correas
Keyword(s):  
Plasma Membrane ◽  
Core Region ◽  
Binding Domain ◽  
Intramolecular Interactions ◽  
Actin Network ◽  
Protein 4.1 ◽  
The Core ◽  
Self Association ◽  
Protein 4.1R

Erythroid protein 4.1 (4.1R) stabilizes the spectrin–actin network and anchors it to the plasma membrane. To contribute to the characterization of non-erythroid protein 4.1R, we used sedimentation, pull-down and co-immunoprecipitation assays to investigate the ability of protein 4.1R to establish inter-/intra-molecular associations. We demonstrated that the small 4.1R isoforms of 60 kDa (4.1R60), but not the larger isoforms of 80 and 135 kDa (4.1R80 and 4.1R135), were self-associated, and that a domain contained in all 4.1R isoforms, the core region, was responsible for 4.1R self-association. Results from denaturing–renaturing experiments, in which an initially non-self-associated 4.1R80 isoform became self-associated, suggested that an initially hidden core region was subsequently exposed. This hypothesis was supported by results from pull-down assays, which showed that the core region interacted with the N-terminal end of the FERM (4.1, ezrin, radixin, moesin) domain that is present in 4.1R80 and 4.1R135 isoforms but absent from 4.1R60 isoforms. Consistently, 4.1R80 isoforms bound neither to each other nor to 4.1R60 isoforms. We propose that 4.1R60 isoforms are constitutively self-associated, whereas 4.1R80 and 4.1R135 self-association is prevented by intramolecular interactions.

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