scholarly journals Laser cross-linking of proteins to nucleic acids. I. Examining physical parameters of protein-nucleic acid complexes

1993 ◽  
Vol 268 (21) ◽  
pp. 15712-15720
Author(s):  
J.W. Hockensmith ◽  
W.L. Kubasek ◽  
W.R. Vorachek ◽  
P.H. von Hippel
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Cross Linking ◽  
Physical Parameters ◽  
Protein Nucleic Acid

scholarly journals Bifunctional cross-linking approaches for mass spectrometry-based investigation of nucleic acids and protein-nucleic acid assemblies

Methods ◽  
2018 ◽  
Vol 144 ◽  
pp. 64-78 ◽  
Author(s):  
M. Scalabrin ◽  
S.M. Dixit ◽  
M.M. Makshood ◽  
C.E. Krzemien ◽  
Daniele Fabris
Keyword(s):  
Mass Spectrometry ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Cross Linking ◽  
Protein Nucleic Acid

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

Schematic diagrams of nucleic acids and protein–nucleic acid complexes

1989 ◽  
Vol 22 (6) ◽  
pp. 569-571 ◽  
Author(s):  
V. I. Lesk ◽  
A. M. Lesk
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Complex Structures ◽  
Acid Complex ◽  
Protein Nucleic Acid ◽  
Schematic Diagrams

Simplified representations of components of nucleic acids have been designed and implemented as programs integrated with other software that draws schematic diagrams of proteins. Examples illustrating the structures of oligonucleotides, tRNA and a protein–nucleic acid complex indicate the utility of these representations for making intelligible illustrations of complex structures containing nucleic acids.

Covalent Binding of Modified Nucleic Acids to Proteins as a Method for Investigation of Specific Protein—Nucleic Acid Interactions

ChemInform ◽  
2005 ◽  
Vol 36 (28) ◽  
Author(s):  
T. S. Zatsepin ◽  
N. G. Dolinnaya ◽  
E. A. Kubareva ◽  
M. G. Ivanovskaya ◽  
V. G. Metelev ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Covalent Binding ◽  
Specific Protein ◽  
Modified Nucleic Acids ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

Covalent binding of modified nucleic acids to proteins as a method for investigation of specific protein–nucleic acid interactions

2005 ◽  
Vol 74 (1) ◽  
pp. 77-95 ◽  
Author(s):  
Timofei S Zatsepin ◽  
N G Dolinnaya ◽  
Elena A Kubareva ◽  
Marina G Ivanovskaya ◽  
V G Metelev ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Covalent Binding ◽  
Specific Protein ◽  
Modified Nucleic Acids ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

α-S-Cysteinylthymine: a model for protein-nucleic acid cross-linking

Biochemistry ◽  
1976 ◽  
Vol 15 (3) ◽  
pp. 463-467 ◽  
Author(s):  
Helen M. Berman ◽  
David E. Zacharias ◽  
Horace L. Carrell ◽  
A. J. Varghese
Keyword(s):  
Nucleic Acid ◽  
Cross Linking ◽  
Protein Nucleic Acid
Sign in / Sign up

Export Citation Format

Share Document