scholarly journals AN APPARATUS AND METHOD FOR THE DETERMINATION OF THE RESPIRATORY QUOTIENT OF SMALL ANIMALS

1927 ◽  
Vol 73 (2) ◽  
pp. 499-506
Author(s):  
Laurence G. Wesson
Keyword(s):  
Respiratory Quotient ◽  
Small Animals

Variation in the respiratory quotient of birds and implications for indirect calorimetry using measurements of carbon dioxide production

1995 ◽  
Vol 198 (1) ◽  
pp. 213-219 ◽  
Author(s):  
G Walsberg ◽  
B Wolf
Keyword(s):  
Carbon Dioxide ◽  
Power Consumption ◽  
Indirect Calorimetry ◽  
Respiratory Quotient ◽  
Current Knowledge ◽  
Bird Species ◽  
Passer Domesticus ◽  
Carbon Dioxide Production ◽  
Accurate Estimation

Determination of animal power consumption by indirect calorimetry relies upon accurate estimation of the thermal equivalent of oxygen consumed or carbon dioxide produced. This estimate is typically based upon measurement or assumption of the respiratory quotient (RQ), the ratio of CO2 produced to O2 consumed. This ratio is used to indicate the mixture of lipids, carbohydrates and proteins in the metabolic substrate. In this analysis, we report the RQ for two bird species, Passer domesticus and Auriparus flaviceps, under several dietary and fasting regimes. RQ commonly differed substantially from those typically assumed in studies of energy metabolism and often included values below those explainable by current knowledge. Errors that could result from these unexpected RQ values can be large and could present the primary limit to the accuracy of power consumption estimates based upon measurement of carbon dioxide production.

Improved liquid-chromatographic determination of caffeine in plasma.

1980 ◽  
Vol 26 (9) ◽  
pp. 1351-1354 ◽  
Author(s):  
J Blanchard ◽  
J D Mohammadi ◽  
K A Conrad
Keyword(s):  
Plasma Sample ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Small Sample ◽  
Small Animals ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We describe a rapid, specific, and sensitive liquid-chromatographic micro-method for caffeine in plasma. Each plasma sample can be assayed within about 15 min of its receipt. Samples are denatured with acetonitrile, centrifuged, and the supernate is chromatographed on a reversed-phase column. Only 100 microL of plasma is required, and concentrations as low as 0.3 mg/L can be measured accurately. Other xanthines and their metabolites do not interfere. The small sample required makes the procedure ideally suited for measuring caffeine in the plasma of infants and small animals as well as adults.

Spectrophotofluorometric Assay for Isoniazid and Acetyl Isoniazid in Plasma Adapted to Pediatric Studies

1977 ◽  
Vol 23 (4) ◽  
pp. 745-748 ◽  
Author(s):  
William A Olson ◽  
Peter G Dayton ◽  
Zafar H Israili ◽  
Albert W Pruitt
Keyword(s):  
Clinical Application ◽  
Small Animals ◽  
Micro Scale ◽  
Acid Labile

Abstract We modified the micro-scale spectrophotofluorometric method of Miceli et al. [Biochem. Med. 12, 348 (1975)] for the assay of "apparent" isoniazid (isoniazid plus acid-labile hydrazones) to improve its clinical application. We also adapted the method for determination of acetyl isoniazid. Data are presented showing how long plasma containing isoniazid may validly be stored. The applicability of the method was demonstrated in studies on children and small animals.

Serial determination of lung volume in small animals by nitrogen washout

1985 ◽  
Vol 59 (1) ◽  
pp. 205-210 ◽  
Author(s):  
T. A. Standaert ◽  
W. A. LaFramboise ◽  
R. E. Tuck ◽  
D. E. Woodrum
Keyword(s):  
Lung Volume ◽  
Small Animals ◽  
Calibration Data ◽  
Water Displacement ◽  
Lung Capacity ◽  
Serial Determination ◽  
Residual Capacity ◽  
Volume Measurements

This report describes the design of an apparatus and the procedures used to serially measure the total lung capacity and the functional residual capacity of small animals utilizing the N2-washout technique. The calibration data indicate that the technique is accurate to within 1 ml and has a variance of less than 5%. The in vivo lung volume measurements of rats were validated by comparing them with values obtained with a water-displacement technique; the means were within 0.3 ml. Examples of the precision and changes in lung volume of animals during studies are included to demonstrate the reliability and usefulness of the technique.

Determination of sulfobromophthalein storage and exertory rate in small animals

1967 ◽  
Vol 22 (6) ◽  
pp. 1151-1155 ◽  
Author(s):  
C. D. Klaassen ◽  
G. L. Plaa
Keyword(s):  
Small Animals
Sign in / Sign up

Export Citation Format

Share Document