scholarly journals Purification of the HhaII restriction endonuclease from an overproducer Escherichia coli clone.

1985 ◽  
Vol 260 (28) ◽  
pp. 15339-15344 ◽  
Author(s):  
S Kelly ◽  
R Kaddurah-Daouk ◽  
H O Smith
Keyword(s):  
Escherichia Coli ◽  
Restriction Endonuclease

scholarly journals Pressure Induced SOS Response in Escherichia coli Involves Mrr Restriction Endonuclease Dissociation

2018 ◽  
Vol 114 (3) ◽  
pp. 152a
Author(s):  
Anais Bourges ◽  
Oscar E. Torres M. ◽  
Anirban Ghosh ◽  
Wubishet Tadesse ◽  
Gilles Labesse ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Restriction Endonuclease ◽  
Sos Response

Cloning and identification of the product of the dnaE gene of Escherichia coli

1982 ◽  
Vol 152 (1) ◽  
pp. 351-356
Author(s):  
M M Welch ◽  
C S McHenry
Keyword(s):  
Escherichia Coli ◽  
Restriction Endonuclease ◽  
Dna Polymerase ◽  
Alpha Subunit ◽  
Temperature Sensitive ◽  
Restriction Map ◽  
Dna Polymerase Iii ◽  
Restriction Endonuclease Site ◽  
E Coli ◽  
A Site

We successively subcloned the dnaE gene of Escherichia coli into pBR322, resulting in a plasmid that contains 4.6 kilobases of E. coli DNA. This plasmid can complement a dnaE temperature-sensitive mutation. A restriction map of the dnaE gene and the surrounding 10.7-kilobase region of the E. coli chromosome was determined. A unique HindIII restriction endonuclease site within the cloned segment of DNA was identified as a site required for expression of the dnaE gene. By using the maxicell plasmid-directed protein synthesizing system, we demonstrated that dnaE codes for the alpha subunit of DNA polymerase III.

Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes

1977 ◽  
Vol 117 (1) ◽  
pp. 175-193 ◽  
Author(s):  
Geoffrey J. Childs ◽  
Hisako Ohtsubo ◽  
Eiichi Ohtsubo ◽  
Frank Sonnenberg ◽  
Martin Freundlich
Keyword(s):  
Escherichia Coli ◽  
Restriction Endonuclease ◽  
Escherichia Coli K12 ◽  
Restriction Endonuclease Mapping ◽  
Endonuclease Mapping
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