A method for the improved resolution of basic amino acids on the automatic amino acid analyzer

1962 ◽  
Vol 9 ◽  
pp. 253-254 ◽  
Author(s):  
David R. Kominz
1973 ◽  
Vol 248 (7) ◽  
pp. 2387-2391 ◽  
Author(s):  
Gladys E. Deibler ◽  
Russell E. Martenson

1976 ◽  
Vol 72 (1-2) ◽  
pp. 305-309
Author(s):  
David E. Peterson ◽  
Nyles W. Charon ◽  
Russell C. Johnson

1965 ◽  
Vol 20 (4) ◽  
pp. 307-312 ◽  
Author(s):  
P. S. Chen ◽  
F. Hanimann

By using the automatic amino acid analyzer (model 120 B, Beckman) the free ninhydrin-positive components in the methanol extracts of Drosophila melanogaster were fractionated. Compared to the conventional paper partition chromatography the great advantage of this technique is the distinct separation of such amino acids like leucine, isoleucine, phenylalanine, valine, methionine, γ-amino-butyric acid as well as the basic components ornithine, lysine, histidine and arginine. Furthermore, the occurrence of phosphoserine, tyrosine phosphate, ethanolamine, phosphoethanolamine and glycerophosphoethanolamine was identified. The metabolic significance of these substances is discussed.


2013 ◽  
Vol 781-784 ◽  
pp. 1528-1533
Author(s):  
Yi Hua Zhang ◽  
Shun Sheng Chen ◽  
Wei Qiang Qiu ◽  
Shou Kun Cheng

The contents of free amino acids(FAAs) in Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis was analyzed by using the automatic amino acid analyzer in this study. The results show that the sequence from the highest to the lowest in total amount of FAAs is Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis. SPSS(19.0) results indicate that except for Cys, Lys and Thr, FAAs in these three shrimps are significant different (p<0.05), and all of them have a high concentration of Arg, Gly and Pro and a low concentration of Asp and Cys. The content of Gly in shrimp is higher than that of crab and oyster. Both Arg and Gly significantly contribute to the taste of the three shrimps, Glu and Pro play an important role in the flavor of Parapenaeopsis hardwickii and Penaeus vannamei, while not as significant as in the taste of Macrobrachium nipponensis. Composition modes of FAAs which make major contributions to flavor in marine shrimps, Parapenaeopsis hardwickii and Penaeus vannamei, are similar, but different from freshwater shrimp, Macrobrachium nipponensis.


1968 ◽  
Vol 48 (3) ◽  
pp. 349-354 ◽  
Author(s):  
F. J. Sowden ◽  
K. C. Ivarson

A system of separating asparagine, glutamine and citrulline from each other and from the other common amino acids, using lithium buffers and the Technicon amino acid analyzer and C2 resin in a 0.63 × 75-cm column, is described. The column was operated with a buffer flow rate of 37 ml/hour at 30 °C for the first 70 min, then at 50 °C. The buffer was 0.067 M in lithium citrate and adjusted to a pH of 2.80; 2% isopropanol was added to improve the separation of threonine and serine. The analysis was complete through citrulline in 4 hours. If a second buffer, pH 4.15, 0.25 N in lithium was added after 3.5 hours, most of the common acidic and neutral amino acids found in soil extracts were separated in 6 hours. Some data on the determination of asparagine and glutamine by amide hydrolysis is included.


1969 ◽  
Vol 101 (11) ◽  
pp. 1207-1213 ◽  
Author(s):  
K. Vick ◽  
W. A. Drew ◽  
J. Young ◽  
E. J. Eisenbraun

AbstractExtracts of free amino acids from six species of ants (Pogonomyrmex barbatus (F. Smith), P. occidentalis (Cresson), Tapinoma sessile (Say), Camponotus pennsylvanicus (DeGeer), Conomyrma pyramica (Roger), and Novomessor cockerelli (E. André) were analyzed by thin layer, one and two dimensional paper chromatography, and an automatic amino acid analyzer to determine the potential use of qualitative and (or) quantitative data of the free amino acid composition of ants in taxonomic studies. The results demonstrate that no significant qualitative differences exist, but that the automatic amino acid analyzer may be used to show significant quantitative differences in the free amino acid composition of the ants studied.


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