Ionenaustausch-chromatographische Untersuchungen über die freien Aminosäuren und deren Derivate in Drosophila melanogaster

1965 ◽  
Vol 20 (4) ◽  
pp. 307-312 ◽  
Author(s):  
P. S. Chen ◽  
F. Hanimann
Keyword(s):  
Amino Acids ◽  
Drosophila Melanogaster ◽  
Amino Acid ◽  
Partition Chromatography ◽  
Automatic Amino Acid Analyzer ◽  
Amino Butyric Acid ◽  
Amino Acid Analyzer ◽  
Tyrosine Phosphate ◽  
Distinct Separation ◽  
Metabolic Significance

By using the automatic amino acid analyzer (model 120 B, Beckman) the free ninhydrin-positive components in the methanol extracts of Drosophila melanogaster were fractionated. Compared to the conventional paper partition chromatography the great advantage of this technique is the distinct separation of such amino acids like leucine, isoleucine, phenylalanine, valine, methionine, γ-amino-butyric acid as well as the basic components ornithine, lysine, histidine and arginine. Furthermore, the occurrence of phosphoserine, tyrosine phosphate, ethanolamine, phosphoethanolamine and glycerophosphoethanolamine was identified. The metabolic significance of these substances is discussed.

Quantitative decarboxylation of [ 14 C]amino acids using automatic amino acid analyzer

1976 ◽  
Vol 72 (1-2) ◽  
pp. 305-309
Author(s):  
David E. Peterson ◽  
Nyles W. Charon ◽  
Russell C. Johnson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Automatic Amino Acid Analyzer ◽  
Amino Acid Analyzer

Comparative Analysis of Free Amino Acids in Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis

2013 ◽  
Vol 781-784 ◽  
pp. 1528-1533
Author(s):  
Yi Hua Zhang ◽  
Shun Sheng Chen ◽  
Wei Qiang Qiu ◽  
Shou Kun Cheng
Keyword(s):  
Amino Acids ◽  
Comparative Analysis ◽  
Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Penaeus Vannamei ◽  
High Concentration ◽  
Automatic Amino Acid Analyzer ◽  
Low Concentration ◽  
Amino Acid Analyzer

The contents of free amino acids(FAAs) in Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis was analyzed by using the automatic amino acid analyzer in this study. The results show that the sequence from the highest to the lowest in total amount of FAAs is Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis. SPSS(19.0) results indicate that except for Cys, Lys and Thr, FAAs in these three shrimps are significant different (p<0.05), and all of them have a high concentration of Arg, Gly and Pro and a low concentration of Asp and Cys. The content of Gly in shrimp is higher than that of crab and oyster. Both Arg and Gly significantly contribute to the taste of the three shrimps, Glu and Pro play an important role in the flavor of Parapenaeopsis hardwickii and Penaeus vannamei, while not as significant as in the taste of Macrobrachium nipponensis. Composition modes of FAAs which make major contributions to flavor in marine shrimps, Parapenaeopsis hardwickii and Penaeus vannamei, are similar, but different from freshwater shrimp, Macrobrachium nipponensis.

DETERMINATION OF ASPARAGINE, GLUTAMINE AND CITRULLINE IN AQUEOUS SOIL EXTRACTS WITH AN AUTOMATIC AMINO ACID ANALYZER

1968 ◽  
Vol 48 (3) ◽  
pp. 349-354 ◽  
Author(s):  
F. J. Sowden ◽  
K. C. Ivarson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
The Other ◽  
Automatic Amino Acid Analyzer ◽  
Soil Extracts ◽  
Amide Hydrolysis ◽  
Amino Acid Analyzer ◽  
Lithium Citrate ◽  
The Common

A system of separating asparagine, glutamine and citrulline from each other and from the other common amino acids, using lithium buffers and the Technicon amino acid analyzer and C2 resin in a 0.63 × 75-cm column, is described. The column was operated with a buffer flow rate of 37 ml/hour at 30 °C for the first 70 min, then at 50 °C. The buffer was 0.067 M in lithium citrate and adjusted to a pH of 2.80; 2% isopropanol was added to improve the separation of threonine and serine. The analysis was complete through citrulline in 4 hours. If a second buffer, pH 4.15, 0.25 N in lithium was added after 3.5 hours, most of the common acidic and neutral amino acids found in soil extracts were separated in 6 hours. Some data on the determination of asparagine and glutamine by amide hydrolysis is included.

CHEMOTAXONOMIC STUDIES OF ANTS: FREE AMINO ACIDS

1969 ◽  
Vol 101 (11) ◽  
pp. 1207-1213 ◽  
Author(s):  
K. Vick ◽  
W. A. Drew ◽  
J. Young ◽  
E. J. Eisenbraun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Free Amino Acid ◽  
Acid Composition ◽  
Free Amino Acids ◽  
Free Amino ◽  
Automatic Amino Acid Analyzer ◽  
Free Amino Acid Composition ◽  
Amino Acid Analyzer

AbstractExtracts of free amino acids from six species of ants (Pogonomyrmex barbatus (F. Smith), P. occidentalis (Cresson), Tapinoma sessile (Say), Camponotus pennsylvanicus (DeGeer), Conomyrma pyramica (Roger), and Novomessor cockerelli (E. André) were analyzed by thin layer, one and two dimensional paper chromatography, and an automatic amino acid analyzer to determine the potential use of qualitative and (or) quantitative data of the free amino acid composition of ants in taxonomic studies. The results demonstrate that no significant qualitative differences exist, but that the automatic amino acid analyzer may be used to show significant quantitative differences in the free amino acid composition of the ants studied.

scholarly journals Determination of Methylated Basic Amino Acids with the Amino Acid Analyzer

1973 ◽  
Vol 248 (7) ◽  
pp. 2387-2391 ◽  
Author(s):  
Gladys E. Deibler ◽  
Russell E. Martenson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Basic Amino Acids ◽  
Amino Acid Analyzer

scholarly journals Evaluation of Amino Acid and Vitamin B2 and E Composition of Genetically Modified Antidwarf Mosaic Maize by Automatic Amino acid Analyzer and HPLC

2013 ◽  
Vol 25 (6) ◽  
pp. 3525-3526 ◽  
Author(s):  
Pingmei Yan ◽  
Qing Wang ◽  
Xiaoyan Yan ◽  
Xiao-Hui Chang ◽  
Jian-Zhong Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetically Modified ◽  
Vitamin B2 ◽  
Automatic Amino Acid Analyzer ◽  
Amino Acid Analyzer

scholarly journals AMINO ACID COMPOSITION OF LEAVES OF THREE SPECIES OF ARTEMISIA L. GROWING IN THE ELTON REGION

2021 ◽  
pp. 219-225
Author(s):  
Галина Табаленкова
Keyword(s):  
Amino Acids ◽  
Czech Republic ◽  
Amino Acid ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Buffer System ◽  
Amino Acid Analyzer ◽  
Growing Conditions ◽  
High Level

The results of studies of the qualitative composition and quantitative content of amino acids (AAs) in the leaves of three plant species of the genus Artemisia, widespread in the Elton region, were presented. Protein AAs were determined on an AAA T-339 amino acid analyzer (Czech Republic) after hydrolysis of a sample in 6N HCl at 105 °C for 24 h, free AAs – on an AAA-400 amino acid analyzer (Czech Republic) in a lithium buffer system. The protein AAs amount varied from 66 mg / g in A. lerchiana to 113 mg / g dry weight in A. santonica. 17 AAs were found in composition of these species, aspartic and glutamic acids were dominant. The content of free AAs varied from 4.4 mg / g in A. santonica to 8.3 mg / g dry weight in A. pauciflora. 14 AAs have been identified, among them proline was the predominant free AA. The share of proline was 75-81% of the total free AAs. Among the minor components, 3-4 compounds with a content above 2% dominated. The free AAs contain 3 non-proteinogenic ones (ornithine, citruline, and γ-aminobutyric acid). A. lerchiana and A. pauciflora species were similar in protein and free amino acids, probably due to the same growing conditions. A high level of free proline, together with a complex of biologically active substances in Artemisia species, which grow abundantly in the Elton region, allow to consider the possibility of their use as a medicinal raw material.

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