High-performance thin-layer chromatography of 2,4-dinitrophenyl-amino acids on layers of RP-a8, RP-18 and ammonium tungstophosphate

1982 ◽  
Vol 235 (2) ◽  
pp. 411-416 ◽  
Author(s):  
Luciano Lepri ◽  
Pier Giorgio Desideri ◽  
Daniela Heimler
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Layer Chromatography ◽  
Ammonium Tungstophosphate

Effects of Echinostoma caproni miracidia dose on the amino acid contents of Biomphalaria glabrata as determined by high-performance thin-layer chromatography

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Mai Nguyen ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biomphalaria Glabrata ◽  
High Dose ◽  
Echinostoma Caproni ◽  
Amino Acid Contents ◽  
Layer Chromatography

AbstractThe effects of 5, 20, and 40 miracidia dose exposures of Echinostoma caproni on the amino acid contents of Biomphalaria glabrata were studied using high performance thin-layer chromatography-densitometry. Amino acids were identified and quantified in whole bodies of exposed snails and in the uninfected matched controls at 2 and 4 weeks post-exposure. Using cellulose layers with the mobile phase 2-butanol-pyridine-glacial acetic acid-deionized water (39:34:10:26) and ninhydrin detection reagent [2% ninhydrin in acetone-n-butanol (1:1)], five amino acids were identified, i.e., leucine/isoleucine, valine, alanine, glycine, and ornithine, by hRF value comparison and color differentiation. Quantitatively, there was a marked elevation in the amounts of four of these five amino acids (isoleucine/leucine, valine, alanine, and ornithine) across dose levels at 4 weeks post-infection (P<0.05). Elevation of the amino acid content in the high dose snail group suggested that some changes occurred in the amino acid metabolism of the snails in that group as a function of miracidia dose.

scholarly journals Amino acid and vitamin determinations by TLC/HPTLC: review of the current state

2012 ◽  
Vol 10 (3) ◽  
pp. 731-750 ◽  
Author(s):  
Ali Mohammad ◽  
Abdul Moheman ◽  
Gaber El-Desoky
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Tabular Form ◽  
Future Prospects ◽  
Pharmaceutical Samples ◽  
Current State ◽  
Layer Chromatography

AbstractSeveral methods to determine amino acids and vitamins in biological and pharmaceutical samples have been reported. Thin layer chromatography (TLC) finds its place when the relatively costly equipment required by other methods is unavailable. This review covers the 1991–2010 literature on TLC/HPTLC (high performance thin layer chromatography) amino acid and vitamin determinations. It gives an overview of the special features as well as the problems in TLC/HPTLC determinations of amino acids and vitamins. Various chromatographic systems useful in amino acid and vitamin identification, separation and quantitation of are presented in tabular form. Future prospects of TLC/HPTLC for amino acid and vitamin determinations are also discussed.

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