Semi-preparative high-performance liquid chromatography and spectroscopic characterisation of eight geometric isomers of leukotriene A methyl ester

1981 ◽  
Vol 214 (2) ◽  
pp. 249-256 ◽  
Author(s):  
S.W. McKay ◽  
D.N.B. Mallen ◽  
P.R. Shrubsall ◽  
J.M. Smith ◽  
S.R. Baker ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Methyl Ester ◽  
High Performance ◽  
Geometric Isomers ◽  
Spectroscopic Characterisation

scholarly journals Identification of ecdysonoic acid and 20-hydroxyecdysonoic acid isolated from developing eggs of Schistocerca gregaria and pupae of Spodoptera littoralis

1983 ◽  
Vol 213 (1) ◽  
pp. 261-265 ◽  
Author(s):  
R E Isaac ◽  
N P Milner ◽  
H H Rees
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Methyl Ester ◽  
Spodoptera Littoralis ◽  
High Performance ◽  
Fast Atom Bombardment ◽  
Schistocerca Gregaria ◽  
Methyl Esters ◽  
Desert Locust

Ecdysonoic acid and 20-hydroxyecdysonoic acid have been purified from developing eggs of the desert locust, Schistocerca gregaria, by high performance liquid chromatography (h.p.l.c.), and their structures were determined by p.m.r. spectroscopy and fast atom bombardment mass spectrometry of the free and methyl ester derivatives. 20-Hydroxyecdysonoic acid was also characterized from Spodoptera littoralis pupae. The occurrence of both 20-hydroxyecdysonoic acid and ecdysonoic acid in Sp. littoralis pupae was also established by h.p.l.c. comparison of the 3H-labelled acids formed from [3H]ecdysone and of their methyl esters with the corresponding substances from Sch. gregaria. The significance of ecdysteroid acids as products of ecdysteroid inactivation is discussed.

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