[18] Separation of retinyl esters and their geometric isomers by isocratic adsorption high-performance liquid chromatography

Author(s):  
Hans K. Biesalski
1986 ◽  
Vol 64 (9) ◽  
pp. 2066-2067 ◽  
Author(s):  
Andrew T. C. Tsin ◽  
Santiago T. Morales Jr. ◽  
Janie M. Flores

Using high-performance liquid chromatography, the quantity of retinol, retinal, and retinyl esters as well as their 3,4-didehydro derivatives were measured in several body compartments and in the remainder of the body of 11 goldfish. Eighty-six percent of these retinoids and 3,4-didehydroretinoids existed as retinyl and 3,4-didehydroretinyl esters, 10% as retinal and 3,4-didehydroretinal, and 4% as retinol and 3,4-didehydroretinol. Most (94%) of these retinoids and 3,4-didehydroretinoids were found in ocular tissues and high ratios of 3,4-didehydroretinoids/retinoids were observed in most tissue extracts. These data suggest that in the goldfish ocular tissues are important locations of retinoid and 3,4-didehydroretinoid metabolism, which favors the formation and (or) storage of 3,4-didehydroretinoids.


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