Combination of zetaprep mass ion-exchange media and high-performance cation-exchange chromatography for the purification of high-purity monoclonal antibodies

1987 ◽  
Vol 397 ◽  
pp. 313-320 ◽  
Author(s):  
A. Jungbauer ◽  
F. Unterluggauer ◽  
F. Steindl ◽  
F. Rüker ◽  
H. Katinger
Keyword(s):  
Monoclonal Antibodies ◽  
Ion Exchange ◽  
Cation Exchange ◽  
High Purity ◽  
High Performance ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography

Effect of temperature on quantifying glycated (glycosylated) hemoglobin by cation-exchange chromatography.

1985 ◽  
Vol 31 (1) ◽  
pp. 114-117 ◽  
Author(s):  
R Flückiger ◽  
T Woodtli
Keyword(s):  
Ionic Strength ◽  
Cation Exchange ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Operating Temperature ◽  
Glycosylated Hemoglobin ◽  
Regression Line ◽  
Exchange Chromatography ◽  
Effect Of Temperature ◽  
Cation Exchange Chromatography

Abstract As a consequence of nonideal chromatographic conditions, values for stable glycated hemoglobin (HbA1c) determined by cation-exchange chromatography in a commercial minicolumn system (y) or by "high-performance" liquid chromatography (x) differ markedly, yielding the regression line y = 0.82x + 0.6. With use of the protocol specified by the manufacturer, 20% of the HbA1c peak is not collected in the HbA1c fraction. Increasing the ionic strength of the eluting buffer by increasing the operating temperature to 28 degrees C increases the rate of elution from the minicolumn, making results of the two methods more closely comparable (y = 0.98x - 0.22). Because at a given pH the elution volume is determined primarily by the ionic strength, close limits on the composition of the eluting buffer are set by the temperature-dependence of its ionic strength. At a specified temperature and pH the position of a peak can be judged to within a volume of 1 mL if the conductivity of the eluent does not vary by more than +/- 0.05 mS.

Cation exchange chromatography performed in overloaded mode is effective in removing viruses during the manufacturing of monoclonal antibodies

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Yumiko Masuda ◽  
Masashi Tsuda ◽  
Chie Hashikawa‐Muto ◽  
Yusuke Takahashi ◽  
Koichi Nonaka ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography

scholarly journals The use of native cation-exchange chromatography to study aggregation and phase separation of monoclonal antibodies

2010 ◽  
Vol 19 (6) ◽  
pp. 1191-1204 ◽  
Author(s):  
Shuang Chen ◽  
Hollis Lau ◽  
Yan Brodsky ◽  
Gerd R. Kleemann ◽  
Ramil F. Latypov
Keyword(s):  
Monoclonal Antibodies ◽  
Phase Separation ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography
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