Application of high-performance liquid chromatography coupled to nuclear magnetic resonance spectrometry, mass spectrometry and bioassay for the determination of active saponins from Bacopa monniera Wettst.

1999 ◽  
Vol 847 (1-2) ◽  
pp. 109-116 ◽  
Author(s):  
T Renukappa ◽  
G Roos ◽  
I Klaiber ◽  
B Vogler ◽  
W Kraus
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
High Performance ◽  
Bacopa Monniera ◽  
Nuclear Magnetic Resonance Spectrometry ◽  
Magnetic Resonance Spectrometry

scholarly journals Transformation of Amoxapine byCunninghamella elegans

2000 ◽  
Vol 66 (8) ◽  
pp. 3646-3649 ◽  
Author(s):  
Joanna D. Moody ◽  
Donglu Zhang ◽  
Thomas M. Heinze ◽  
Carl E. Cerniglia
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
High Performance ◽  
Tricyclic Antidepressant ◽  
Cunninghamella Elegans ◽  
Toxicological Evaluation

ABSTRACT We examined Cunninghamella elegans to determine its ability to transform amoxapine, a tricyclic antidepressant belonging to the dibenzoxazepine class of drugs. Approximately 57% of the exogenous amoxapine was metabolized to three metabolites that were isolated by high-performance liquid chromatography and were identified by nuclear magnetic resonance and mass spectrometry as 7-hydroxyamoxapine (48%),N-formyl-7-hydroxyamoxapine (31%), andN-formylamoxapine (21%). 7-Hydroxyamoxapine, a mammalian metabolite with biological activity, now can be produced in milligram quantities for toxicological evaluation.

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