Transformation of Amoxapine byCunninghamella elegans

ABSTRACT We examined Cunninghamella elegans to determine its ability to transform amoxapine, a tricyclic antidepressant belonging to the dibenzoxazepine class of drugs. Approximately 57% of the exogenous amoxapine was metabolized to three metabolites that were isolated by high-performance liquid chromatography and were identified by nuclear magnetic resonance and mass spectrometry as 7-hydroxyamoxapine (48%),N-formyl-7-hydroxyamoxapine (31%), andN-formylamoxapine (21%). 7-Hydroxyamoxapine, a mammalian metabolite with biological activity, now can be produced in milligram quantities for toxicological evaluation.

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High performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy and mass spectrometry applied to plant products: Identification of ecdysteroids fromSilene otites

Chromatographia ◽

10.1007/bf02467609 ◽

1999 ◽

Vol 49 (7-8) ◽

pp. 374-378 ◽

Cited By ~ 39

Author(s):

I. D. Wilson ◽

E. D. Morgan ◽

R. Lafont ◽

J. P. Shockcor ◽

J. C. Lindon ◽

...

Keyword(s):

Mass Spectrometry ◽

Nuclear Magnetic Resonance ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Magnetic Resonance ◽

Magnetic Resonance Spectroscopy ◽

Nuclear Magnetic Resonance Spectroscopy ◽

High Performance ◽

Resonance Spectroscopy ◽

Plant Products

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Authentic compound-free strategy for simultaneous determination of primary coumarins in Peucedani Radix using offline high performance liquid chromatography–nuclear magnetic resonance spectroscopy–tandem mass spectrometry

Acta Pharmaceutica Sinica B ◽

10.1016/j.apsb.2018.01.005 ◽

2018 ◽

Vol 8 (4) ◽

pp. 645-654 ◽

Cited By ~ 7

Author(s):

Yao Liu ◽

Qingqing Song ◽

Wenjing Liu ◽

Peng Li ◽

Jun Li ◽

...

Keyword(s):

Mass Spectrometry ◽

Nuclear Magnetic Resonance ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Magnetic Resonance ◽

High Performance ◽

Resonance Spectroscopy ◽

Tandem Mass ◽

Authentic Compound

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Fungal Biotransformation of 6-Nitrochrysene

Applied and Environmental Microbiology ◽

10.1128/aem.64.8.3106-3109.1998 ◽

1998 ◽

Vol 64 (8) ◽

pp. 3106-3109 ◽

Cited By ~ 16

Author(s):

Jairaj V. Pothuluri ◽

John B. Sutherland ◽

James P. Freeman ◽

Carl E. Cerniglia

Keyword(s):

Nuclear Magnetic Resonance ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Magnetic Resonance ◽

High Performance ◽

Environmental Contaminant ◽

Cunninghamella Elegans ◽

Spectral Techniques ◽

Mass Spectral ◽

Uv Visible

ABSTRACT The fungus Cunninghamella elegans was used to biotransform 6-nitrochrysene, a mutagen that is a widespread environmental contaminant. After 6 days, 74% of the3H-labeled 6-nitrochrysene added had been metabolized to two isomeric sulfate conjugates. These conjugates were separated by high-performance liquid chromatography and identified by UV-visible, 1H nuclear magnetic resonance, and mass spectral techniques as 6-nitrochrysene 1-sulfate and 6-nitrochrysene 2-sulfate.

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