scholarly journals Tracking cell cholesterol with cholesterol oxidase.

1992 ◽  
Vol 33 (3) ◽  
pp. 315-321
Author(s):  
Y Lange
Keyword(s):  
Biochemistry ◽  
1977 ◽  
Vol 16 (21) ◽  
pp. 4708-4715 ◽  
Author(s):  
Norman F. Moore ◽  
Eric J. Patzer ◽  
Yechezkel Barenholz ◽  
Robert R. Wagner

1979 ◽  
Vol 254 (11) ◽  
pp. 4689-4690 ◽  
Author(s):  
W.C. Kenney ◽  
T.P. Singer ◽  
M. Fukuyama ◽  
Y. Miyake

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3368 ◽  
Author(s):  
Kefilwe Mokwebo ◽  
Oluwatobi Oluwafemi ◽  
Omotayo Arotiba

We report the preparation of poly (propylene imine) dendrimer (PPI) and CdTe/CdSe/ZnSe quantum dots (QDs) as a suitable platform for the development of an enzyme-based electrochemical cholesterol biosensor with enhanced analytical performance. The mercaptopropionic acid (MPA)-capped CdTe/CdSe/ZnSe QDs was synthesized in an aqueous phase and characterized using photoluminescence (PL) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), X-ray power diffraction (XRD), energy dispersive X-ray (EDX) spectroscopy. The absorption and emission maxima of the QDs red shifted as the reaction time and shell growth increased, indicating the formation of CdTe/CdSe/ZnSe QDs. PPI was electrodeposited on a glassy carbon electrode followed by the deposition (by deep coating) attachment of the QDs onto the PPI dendrimer modified electrode using 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) as a coupling agent. The biosensor was prepared by incubating the PPI/QDs modified electrode into a solution of cholesterol oxidase (ChOx) for 6 h. The modified electrodes were characterized by voltammetry and impedance spectroscopy. Since efficient electron transfer process between the enzyme cholesterol oxidase (ChOx) and the PPI/QDs-modified electrode was achieved, the cholesterol biosensor (GCE/PPI/QDs/ChOx) was able to detect cholesterol in the range 0.1–10 mM with a detection limit (LOD) of 0.075 mM and sensitivity of 111.16 μA mM−1 cm−2. The biosensor was stable for over a month and had greater selectivity towards the cholesterol molecule.


1979 ◽  
Vol 12 (14) ◽  
pp. 1477-1488 ◽  
Author(s):  
Catherine Bertrand ◽  
Pierre R. Coulet ◽  
Danièle C. Gautheron

2021 ◽  
Vol 15 (7) ◽  
pp. 1497-1499
Author(s):  
Rao. S Aziz ◽  
Usman Saeed ◽  
Liaqat Ali ◽  
Muhammad Arshad ◽  
Roman Abbas ◽  
...  

Background: Valproic acid (VA) serve as the antimigraine , anti-mental disturbances agent and antiepileptic medicine. After using va, metabolic rearrangements seen in patients that include alteration in lipoproteins levels; Aim: To discuss the effects of VA after using for long duration on total levels of cholesterol in adult. Methods: About Eighty candidates participated and they were divided into two groups namely, case group (40 candidates) and control groups (40 candidates). All the candidates were asked for collection of venous blood sample in order to determine total cholesterol serum level among them via aid of enzymatic cholesterol oxidase phenol 4-aminoantipyrine peroxidase. Results: By the aid of the logistic regression analysis, the relationship of the long-term VA treatment and the level of total cholesterol was obtained. With respect to our analysis, there is a co relation between total levels of cholestrol and long term usage of VA (P=0.003). Conclusions: In a net shell, by using VA for long duration, the total level of cholesterol in adults reduces. Keywords: Lipid profile, side effects, total cholesterol, valproic acid


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