REAL-TIME TISSUE RECOGNITION OF PERIPROSTATIC TISSUE ARCHITECTURE AND ITS RELEVANCE T O NERVE SPARING RADICAL PROSTATECTOMY: A PILOT STUDY USING MULTIPHOTON MICROSCOPY AND SECOND HARMONIC GENERATION

2009 ◽  
Vol 181 (4) ◽  
pp. 670-671
Author(s):  
Rajiv Yadav ◽  
Michael Herman ◽  
Sushmita Mukherjee ◽  
Philip J Dorsey ◽  
Gerald Y Tan ◽  
...  
Keyword(s):  
Pilot Study ◽  
Radical Prostatectomy ◽  
Second Harmonic Generation ◽  
Real Time ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Nerve Sparing ◽  
Tissue Architecture

scholarly journals MULTIPHOTON MICROSCOPIC IMAGING OF MOUSE INTESTINAL MUCOSA BASED ON TWO-PHOTON EXCITED FLUORESCENCE AND SECOND HARMONIC GENERATION

2013 ◽  
Vol 06 (01) ◽  
pp. 1350004 ◽  
Author(s):  
REN'AN XU ◽  
XIAOQIN ZHU ◽  
NING HE ◽  
SHUANGMU ZHUO ◽  
JIAN XU ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Tissue Architecture ◽  
Intestinal Tissue ◽  
Two Photon ◽  
Main Components ◽  
Columnar Cells

Multiphoton microscopy (MPM), based on two-photon excited fluorescence and second harmonic generation, enables direct noninvasive visualization of tissue architecture and cell morphology in live tissues without the administration of exogenous contrast agents. In this paper, we used MPM to image the microstructures of the mucosa in fresh, unfixed, and unstained intestinal tissue of mouse. The morphology and distribution of the main components in mucosa layer such as columnar cells, goblet cells, intestinal glands, and a little collagen fibers were clearly observed in MPM images, and then compared with standard H&E images from paired specimens. Our results indicate that MPM combined with endoscopy and miniaturization probes has the potential application in the clinical diagnosis and in vivo monitoring of early intestinal cancer.

scholarly journals Strong second harmonic generation in SiC, ZnO, GaN two-dimensional hexagonal crystals from first-principles many-body calculations

2015 ◽  
Vol 17 (14) ◽  
pp. 9533-9540 ◽  
Author(s):  
C. Attaccalite ◽  
A. Nguer ◽  
E. Cannuccia ◽  
M. Grüning
Keyword(s):  
Second Harmonic Generation ◽  
Real Time ◽  
Harmonic Generation ◽  
First Principles ◽  
Function Theory ◽  
Second Harmonic ◽  
Two Dimensional ◽  
Many Body ◽  
Ti:Sapphire Laser ◽  
Hexagonal Crystals

By using a real-time approach based on Green's function theory we predict a strong second-harmonic generation (SHG) for frequencies at which Ti:sapphire laser operates and for which the materials are transparent.

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