550: Time Course of Nicotinamide Adenine Dinucleotide Diaphorase Staining Following Renal Radiofrequency Ablation Influences Viability Assessment

2006 ◽  
Vol 175 (4S) ◽  
pp. 178-179
Author(s):  
J. Kyle Anderson ◽  
Meredith R. Baker ◽  
Omar Jaffer ◽  
Margaret S. Pearle ◽  
Jeffrey A. Cadeddu
2007 ◽  
Vol 21 (2) ◽  
pp. 223-227 ◽  
Author(s):  
J. Kyle Anderson ◽  
Meredith Baker ◽  
Omar Jaffers ◽  
Margaret S. Pearle ◽  
Guy L. Lindberg ◽  
...  

1967 ◽  
Vol 20 (2) ◽  
pp. 319 ◽  
Author(s):  
D Graham ◽  
Judith E Cooper

Changes in levels of nicotinamide adenine nucleotides were measured during a short (30 min) period of illumination following a dark period. Two phases in the time course were found. In the first phase, during the first minute of illumination, a rapid decline in oxidized nicotinamide adenine dinucleotide occurred which represented a net loss of nicotinamide adenine nucleotide. In the subsequent, second phase during illumination, a slower decline in oxidized nicotinamide adenine dinucleotide was found which was coincident with increases in nicotinamide adenine dinucleotide phosphate(s). The changes in the reduced nicotinamide adenine nucleo� tides were relatively small during illumination.


Author(s):  
M. Arif Hayat

Although it is recognized that niacin (pyridine-3-carboxylic acid), incorporated as the amide in nicotinamide adenine dinucleotide (NAD) or in nicotinamide adenine dinucleotide phosphate (NADP), is a cofactor in hydrogen transfer in numerous enzyme reactions in all organisms studied, virtually no information is available on the effect of this vitamin on a cell at the submicroscopic level. Since mitochondria act as sites for many hydrogen transfer processes, the possible response of mitochondria to niacin treatment is, therefore, of critical interest.Onion bulbs were placed on vials filled with double distilled water in the dark at 25°C. After two days the bulbs and newly developed root system were transferred to vials containing 0.1% niacin. Root tips were collected at ¼, ½, 1, 2, 4, and 8 hr. intervals after treatment. The tissues were fixed in glutaraldehyde-OsO4 as well as in 2% KMnO4 according to standard procedures. In both cases, the tissues were dehydrated in an acetone series and embedded in Reynolds' lead citrate for 3-10 minutes.


1967 ◽  
Vol 28 (2) ◽  
pp. 213-224 ◽  
Author(s):  
E. Majchrowicz ◽  
B. L. Bercaw ◽  
W. M. Cole ◽  
D. H. Gregory

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