Follicle Selection in Cyclic Guinea Pigs with Active Immunization Against Inhibin α-Subunit

Life Sciences ◽  
2000 ◽  
Vol 66 (21) ◽  
pp. 2489-2497 ◽  
Author(s):  
F Shi
Keyword(s):  
Guinea Pigs ◽  
Active Immunization ◽  
Α Subunit ◽  
Follicle Selection

Follicle selection in cyclic guinea pigs with active immunization against inhibin α-subunit

Life Sciences ◽  
2000 ◽  
Vol 66 (25) ◽  
pp. 2489-2497 ◽  
Author(s):  
FangXiong Shi ◽  
Keiji Mochida ◽  
Atsuo Ogura ◽  
Junichiro Matsuda ◽  
Osamu Suzuki ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Active Immunization ◽  
Α Subunit ◽  
Follicle Selection

scholarly journals STUDIES ON TYPHUS FEVER

1931 ◽  
Vol 53 (4) ◽  
pp. 493-497 ◽  
Author(s):  
Hans Zinsser ◽  
M. Ruiz Castaneda
Keyword(s):  
Guinea Pigs ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Active Immunization ◽  
Rocky Mountain Spotted Fever ◽  
Typhus Fever

Guinea pigs can be immunized against Mexican typhus virus by peritoneal injections of formalinized Rickettsia material, provided sufficient amounts of the organisms are used. Our results in this respect are analogous to those of Spencer and Parker with carbolized virus of Rocky Mountain spotted fever. The Rickettsia suspensions appear to possess considerable toxicity. We do not wish to be misunderstood as implying that the results in guinea pigs offer anything more than a demonstration of the principle of active immunization with killed Rickettsiae. Application to man will have to be worked out, and preliminary to this, we are now attempting to apply the methods to a limited number of monkeys.

Active Immunization of Mares Against the Recombinant Human Inhibin α-subunit

1997 ◽  
Vol 32 (5) ◽  
pp. 243-250 ◽  
Author(s):  
H-J Terhaar ◽  
S Schlote ◽  
H-O Hoppen ◽  
M Hennies ◽  
W Holtz ◽  
...  
Keyword(s):  
Active Immunization ◽  
Α Subunit

scholarly journals Active immunization against GnRH in pre-pubertal domestic mammals: testicular morphometry, histopathology and endocrine responses in rabbits, guinea pigs and ram lambs

animal ◽  
2018 ◽  
Vol 12 (4) ◽  
pp. 784-793 ◽  
Author(s):  
P.M. Aponte ◽  
M.A. Gutierrez-Reinoso ◽  
E.G. Sanchez-Cepeda ◽  
M. Garcia-Herreros
Keyword(s):  
Guinea Pigs ◽  
Active Immunization ◽  
Ram Lambs

Effect of active immunization of heifers against inhibin on plasma FSH concentrations, ovarian follicular development and ovulation rate

1992 ◽  
Vol 134 (1) ◽  
pp. 11-18 ◽  
Author(s):  
R. G. Glencross ◽  
E. C. L. Bleach ◽  
B. J. McLeod ◽  
A. J. Beard ◽  
P. G. Knight
Keyword(s):  
Synthetic Peptide ◽  
Ovarian Function ◽  
Statistical Significance ◽  
Follicular Development ◽  
Ovarian Response ◽  
Active Immunization ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Α Subunit ◽  
Ovarian Follicular Development

ABSTRACT To study the effects of immunoneutralization of endogenous inhibin on gonadotrophin secretion and ovarian function, prepubertal heifers (n = 6) were actively immunized against a synthetic peptide replica of the N-terminal sequence of bovine inhibin α subunit bIα(1–29)Tyr30) coupled to ovalbumin. In contrast to ovalbumin-immunized controls (n=6), bIα(1–29)Tyr30-immunized heifers had detectable inhibin antibody titres (% binding to 125I-labelled bovine inhibin at 1:2000 dilution of plasma) of 17 ± 3% (s.e.m.) at puberty, rising to 31 ± 5% by the end of the study period 7 months later. Neither age (immunized: 295 ± 8 days; controls: 300 ± 5 days) nor body weight (immunized: 254 ± 13 kg; controls 251 ± 9 kg) at onset of puberty differed between groups. Although the difference did not reach statistical significance, mean plasma FSH concentrations recorded in inhibin-immunized heifers remained 35–40% higher than in controls throughout the 12-week period leading up to puberty (P = 0·14) and during nine successive oestrous cycles studied after puberty (P=0·10). Plasma LH concentrations did not differ between groups at any time during the study. Inhibin immunization had no effect on oestrous cycle length (immunized: 19·8±0·5 days; controls: 19·9±0·5 days). However, in comparison with controls, inhibinimmunized heifers had more medium sized (≥0·5 to <1 cm diameter) follicles during both the preovulatory (95%, P<0·001) and post-ovulatory (110%, P < 0·05 waves of follicular growth and more large (>1 cm diameter) follicles during the preovulatory wave (49%, P<0·05). In addition, the number of corpora lutea observed during the post-ovulatory phase of each cycle was significantly greater in the inhibin-immunized group (43%, P<0·01), as was the recorded incidence of cycles with multiple ovulations (19/56 in the inhibin-immunized group compared with 0/54 in controls; P<0·001). All six inhibinimmunized heifers had at least one cycle with multiple ovulation whereas none of the control heifers did so. These results support the conclusion that immunoneutralization of endogenous inhibin using a synthetic peptide-based vaccine can enhance ovarian follicular development and ovulation rate in heifers. Whether this ovarian response is dependent upon the expected increase in secretion of FSH remains to be established. Journal of Endocrinology (1992) 134, 11–18

Molecular basis of species-specific expression of repolarizing K+ currents in the heart

2003 ◽  
Vol 285 (4) ◽  
pp. H1641-H1649 ◽  
Author(s):  
Stephen Zicha ◽  
Isaac Moss ◽  
Bruce Allen ◽  
Andras Varro ◽  
Julius Papp ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Protein Expression ◽  
Guinea Pigs ◽  
Delayed Rectifier ◽  
Important Species ◽  
Α Subunit ◽  
Delayed Rectifier Current ◽  
Mrna Concentration ◽  
Subunit Expression ◽  
Species Specific

There are important species-specific differences in K+ current profiles and arrhythmia susceptibility, but interspecies comparisons of K+ channel subunit expression are lacking. We quantified voltage-gated K+ channel (Kv) subunit mRNA and protein in rabbits, guinea pigs, and humans. Kv1.4, Kv4.2, and Kv4.3 mRNA was present in rabbits but undetectable in guinea pigs. MinK mRNA concentration in guinea pigs was almost threefold greater versus humans and 20-fold versus rabbits. MinK protein expression in guinea pigs was almost twofold that in humans and sixfold that in rabbits. KvLQT1 mRNA concentration was greatest in humans, and protein expression in humans was increased by ∼2- and ∼7-fold compared with values in rabbits and guinea pigs, respectively. The ether-a-go-go-related gene (ERG1) mRNA was more concentrated in humans, but ERG1 protein expression could not be compared across species because of epitope sequence differences. We conclude that important interspecies differences in cardiac K+ channel subunit expression exist and may contribute to the following: 1) lack of a transient outward current in the guinea pig (α-subunit transcription absent in the guinea pig heart); 2) small slow delayed rectifier current and torsades de pointes susceptibility in the rabbit (low-level minK expression); and 3) large slow component of the delayed rectifier current in the guinea pig (strong minK expression).

scholarly journals The Active Immunization of Guinea-Pigs Passively Immunized with Homologous Antitoxic Serum

1955 ◽  
Vol 53 (2) ◽  
pp. 172-179 ◽  
Author(s):  
J. H. Mason ◽  
Mary Robinson ◽  
P. Agerholm Christensen
Keyword(s):  
Guinea Pigs ◽  
Diphtheria Toxin ◽  
Active Immunization ◽  
The Other ◽  
Control Groups ◽  
Large Measure ◽  
Primary Stimulus ◽  
Serum Titre ◽  
Secondary Stimulus

SummaryGroups of guinea-pigs were passively immunized against diphtheria toxin with homologous antitoxic serum so that their sera contained, at the start of the experiment, 1·0, 0·1, 0·01 or 0·001 unit/ml, respectively. They were then actively immunized with one, or two spaced, injections of 0·1 Lf of A.D.F. Two control groups were included, one passively immunized only and the other actively immunized only.Passively produced serum titres of 0·01 and 0·001 unit/ml. did not interfere with active immunization in any demonstrable way.A titre of 0·1 unit/ml. did interfere with active immunization, markedly 4 weeks after the primary, slightly 2 weeks after the secondary, and markedly 14 weeks after the secondary, stimulus.A titre of 1·0 unit/ml. interfered with active immunization, markedly 4 weeks after the primary, and 2 and 14 weeks after the secondary, stimulus. This titre, however, did not completely annul the effect of the primary stimulus. The highest observed serum titre was obtained at the 32nd, instead of at the 4th week, as in the guinea-pigs actively immunized only.In large measure the results confirm those of Barr and her colleagues who found that, in human babies, an initial ‘passive’ titre of 0·04 unit/ml. serum did not interfere with active immunization, whereas a titre of 0·1 unit/ml, led to unsatisfactory immunization.

scholarly journals ACTIVE IMMUNIZATION OF GUINEA PIGS WITH THE VIRUS OF EQUINE ENCEPHALOMYELITIS

1936 ◽  
Vol 63 (5) ◽  
pp. 745-765 ◽  
Author(s):  
Herald R. Cox ◽  
Peter K. Olitsky
Keyword(s):  
Induced Resistance ◽  
Guinea Pigs ◽  
Quantitative Methods ◽  
Active Immunization ◽  
Active Virus ◽  
High Degree

From a study by quantitative methods, the conclusion is reached that a resistance of high degree may be induced in guinea pigs and mice against experimental equine encephalomyelitis by means of formolized vaccines in which no active virus can be demonstrated. The induced resistance is not due to residual traces of active virus which might possibly have escaped detection in the formolized tissue preparations.

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