Nitric oxide (NO) synthase mRNA expression and NO production via muscarinic acetylcholine receptor-mediated pathways in the CEM, human leukemic T-cell line

Life Sciences ◽  
2003 ◽  
Vol 72 (18-19) ◽  
pp. 2151-2154 ◽  
Author(s):  
Yuichiro Kamimura ◽  
Takeshi Fujii ◽  
Hirotatsu Kojima ◽  
Tetsuo Nagano ◽  
Koichiro Kawashima
Keyword(s):  
Nitric Oxide ◽  
T Cell ◽  
Mrna Expression ◽  
Cell Line ◽  
Acetylcholine Receptor ◽  
Muscarinic Acetylcholine Receptor ◽  
No Synthase ◽  
No Production ◽  
Muscarinic Acetylcholine

Inducible Activity of Ginger Rhizome (Zingiber Offifinale Rosc.) on the mRNA Expression of Macrophage-Inducible Nitric Oxide (NO) Synthase and NO Production in a Macrophage Cell Line, RAW264.7 Cells

2004 ◽  
Vol 32 (05) ◽  
pp. 727-735 ◽  
Author(s):  
Nobuko Imanishi ◽  
Naoki Mantani ◽  
Shinya Sakai ◽  
Miyuki Sato ◽  
Yuko Katada ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Kinetic Studies ◽  
No Synthase ◽  
Raw264.7 Cells ◽  
No Production ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Inducible Nitric Oxide

We have investigated the effect of Zingiber offifinale Rosc. (ZOR) on macrophage-inducible nitric oxide (NO) synthase (macNOS) mRNA expression and NO production in RAW264.7 cells, a murine macrophage cell line; 100 μg/ml ZOR can induce macNOS mRNA expression, but induction effects at a dose below 10 μg/ml were weak or negligible. Kinetic studies showed that macNOS mRNA can be detected from 4 hours to 24 hours after dosing, with a peak at 8 hours. In accordance with the induction of macNOS mRNA expression, NO concentrations increased from 3.4 μM at 2 hours to almost 150 μM at 24 hours, reflecting a longer period of macNOS mRNA expression. The activity of ZOR can be considered to contribute, at least in part, to the beneficial effects of ZOR through the macNOS-mediated activation of the biodefense mechanism.

Functional expression of a cloned Drosophila muscarinic acetylcholine receptor in a stable Drosophila cell line.

1995 ◽  
Vol 198 (9) ◽  
pp. 1843-1850
Author(s):  
N S Millar ◽  
H A Baylis ◽  
C Reaper ◽  
R Bunting ◽  
W T Mason ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Cell Line ◽  
Acetylcholine Receptor ◽  
Muscarinic Acetylcholine Receptor ◽  
Functional Expression ◽  
Apparent Molecular Mass ◽  
Drosophila Cell ◽  
Muscarinic Antagonist ◽  
Muscarinic Acetylcholine ◽  
Drosophila Cell Line

A cloned Drosophila muscarinic acetylcholine receptor (mAChR) has been stably expressed in a Drosophila cell line (S2) under the control of an inducible Drosophila metallothionein promoter. A clonal cell line (S2-Dm1-1) has been isolated which, after induction of mAChR expression with CuSO4, exhibits high-affinity, saturable, specific binding of the muscarinic antagonist N-methyl scopolamine (NMS). The apparent molecular mass of the expressed protein, calculated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), is in good agreement with the apparent molecular mass of mAChRs purified from Drosophila brain. Functional expression of the cloned mAChR in this stable cell line has been demonstrated by quantitative fluorescence ratio-imaging of Fura-2-loaded cells. We have observed transient, agonist-induced elevations in intracellular Ca2+ levels which can be completely blocked by atropine, whereas AFDX-116, a muscarinic antagonist which binds preferentially to the vertebrate mAChR M2 subtype, has little effect at 100 mumol l-1. The suitability of this stable Drosophila expression system for the characterization of neurotransmitter receptors is discussed.

Enhanced Expression of Inducible Nitric Oxide Synthase by Juzen-Taiho-To in LPS-Activated RAW264.7 Cells, a Murine Macrophage Cell Line

2000 ◽  
Vol 28 (02) ◽  
pp. 217-226 ◽  
Author(s):  
Hiroshi Kawamata ◽  
Hiroshi Ochiai ◽  
Naoki Mantani ◽  
Katsutoshi Terasawa
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Raw264.7 Cells ◽  
Inos Mrna ◽  
No Production ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

We have investigated the effect of Juzen-taiho-to (TJ-48) on inducible NO synthase (iNOS) expression and nitric oxide (NO) production in RAW264.7 cells, a murine macrophage cell line. TJ-48-lipopolysaccharide (LPS) combination induced iNOS mRNA expression earlier, stronger and remained longer that paralleled but with a higher NO production compared to LPS stimulation. TJ-48 itself showed no inducible effect either no NO production or iNOS mRNA expression. This phenomenon could be considered to contribute, at least in part, to the beneficial effects of TJ-48 through the iNOS-mediated activation of biodefense mechanism.

Muscarinic acetylcholine receptor subtype mRNA expression and ligand binding in the aged rat forebrain

1991 ◽  
Vol 12 (3) ◽  
pp. 193-199 ◽  
Author(s):  
Michael J. Blake ◽  
Nathan M. Appel ◽  
James A. Joseph ◽  
Carol A. Stagg ◽  
Mike Anson ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Mrna Expression ◽  
Acetylcholine Receptor ◽  
Muscarinic Acetylcholine Receptor ◽  
Receptor Subtype ◽  
Aged Rat ◽  
Muscarinic Acetylcholine ◽  
Rat Forebrain
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