P III A.10 Influence of nuclear DNA polymerases and MSH3 gene on adaptive mutations in yeast Saccharomyces cerevisiae

1997 ◽  
Vol 379 (1) ◽  
pp. S20
Author(s):  
Agnieszka Halas ◽  
Hanna Baranowska ◽  
Zofia Policinska ◽  
Witold Jachymczyk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Dna ◽  
Dna Polymerases ◽  
Yeast Saccharomyces Cerevisiae ◽  
Adaptive Mutations

scholarly journals The essential DNA polymerases delta and epsilon are involved in repair of UV-damaged DNA in the yeast Saccharomyces cerevisiae.

1999 ◽  
Vol 46 (2) ◽  
pp. 289-298 ◽  
Author(s):  
A Hałas ◽  
Z Policińska ◽  
H Baranowska ◽  
W J Jachymczyk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Uv Irradiation ◽  
Dna Polymerases ◽  
Relative Molecular Mass ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Mutant Strains ◽  
Cellular Dna

We have studied the ability of yeast DNA polymerases to carry out repair of lesions caused by UV irradiation in Saccharomyces cerevisiae. By the analysis of postirradiation relative molecular mass changes in cellular DNA of different DNA polymerases mutant strains, it was established that mutations in DNA polymerases delta and epsilon showed accumulation of single-strand breaks indicating defective repair. Mutations in other DNA polymerase genes exhibited no defects in DNA repair. Thus, the data obtained suggest that DNA polymerases delta and epsilon are both necessary for DNA replication and for repair of lesions caused by UV irradiation. The results are discussed in the light of current concepts concerning the specificity of DNA polymerases in DNA repair.

Protection of nuclear DNA by lifespan-extending compounds in the yeast Saccharomyces cerevisiae

2021 ◽  
Vol 822 ◽  
pp. 111738
Author(s):  
Wei-Hsuan Su ◽  
Christelle E.T. Chan ◽  
Ting Lian ◽  
Mareena Biju ◽  
Ayaka Miura ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Dna ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals The dynamics of chromosome movement in the budding yeast Saccharomyces cerevisiae.

1989 ◽  
Vol 109 (6) ◽  
pp. 3355-3366 ◽  
Author(s):  
R E Palmer ◽  
M Koval ◽  
D Koshland
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Dna ◽  
Live Cells ◽  
Chromosome Movement ◽  
Wild Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chromosome Separation ◽  
Higher Eukaryotes ◽  
Spindle Position ◽  
Rate Of Movement

Nuclear DNA movement in the yeast, Saccharomyces cerevisiae, was analyzed in live cells using digital imaging microscopy and corroborated by the analysis of nuclear DNA position in fixed cells. During anaphase, the replicated nuclear genomes initially separated at a rate of 1 micron/min. As the genomes separated, the rate of movement became discontinuous. In addition, the axis defined by the segregating genomes rotated relative to the cell surface. The similarity between these results and those previously obtained in higher eukaryotes suggest that the mechanism of anaphase movement may be highly conserved. Before chromosome separation, novel nuclear DNA movements were observed in cdc13, cdc16, and cdc23 cells but not in wild-type or cdc20 cells. These novel nuclear DNA movements correlated with variability in spindle position and length in cdc16 cells. Models for the mechanism of these movements and their induction by certain cdc mutants are discussed.

scholarly journals ADAPTIVE MUTAGENESIS IN THE YEAST SACCHAROMYCES CEREVISIAE

2006 ◽  
Vol 4 (3) ◽  
pp. 20-28 ◽  
Author(s):  
Nora Babudri ◽  
Angela Lucaccioni ◽  
Alessandro Achilli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Mechanisms ◽  
Random Mutagenesis ◽  
Selective Advantage ◽  
Proliferating Cells ◽  
Yeast Saccharomyces Cerevisiae ◽  
Adaptive Mutagenesis ◽  
Adaptive Mutations ◽  
Experimental Systems ◽  
Adverse Environmental Conditions

The nature of mutation in microorganisms has been debated for a long time. Two theories have been at odds: random spontaneous mutagenesis vs. adaptive mutagenesis. "random mutagenesis" means that mutations occur in proliferating cells before they encountered the selective agent. "adaptive mutagenesis" means that advantageous mutations form in the environment where they have been selected, in non-replicating or poorly replicating cells even though other, non-selected, mutations occur at the same time. In the last 20 years it has been definitely shown that random as well as adaptive mutagenesis occur in bacteria and yeast. microorganisms in nature do not divide or divide poorly because of adverse environmental conditions; therefore adaptive mutations could provide cells with a selective advantage and allow evolution of populations. Here we will focus on some fundamental aspects of adaptive mutagenesis in the yeast Saccharomyces cerevisiae. We begin with a historical overview on the nature of mutation. We then focus on experimental systems aimed at proving or disproving adaptive mutagenesis. We have briefly summarized the results obtained in this field, with particular attention to genetic and molecular mechanisms.

scholarly journals Participation of translesion synthesis DNA polymerases in the maintenance of chromosome integrity in yeast Saccharomyces cerevisiae

2011 ◽  
Vol 76 (1) ◽  
pp. 49-60
Author(s):  
O. V. Kochenova ◽  
J. V. Soshkina ◽  
E. I. Stepchenkova ◽  
S. G. Inge-Vechtomov ◽  
P. V. Shcherbakova
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chromosome Integrity
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