Cellulose acetate and acrylamide gel electrophoresis of serum lipoproteins

Pathology ◽  
1972 ◽  
Vol 4 (2) ◽  
pp. 156
Author(s):  
J. Green
Keyword(s):  
Cellulose Acetate ◽  
Gel Electrophoresis ◽  
Serum Lipoproteins

scholarly journals A Simple Method for Separating Serum Lipoproteins by Electrophoresis on Cellulose Acetate

1970 ◽  
Vol 16 (5) ◽  
pp. 362-365 ◽  
Author(s):  
Margaret J Fletcher ◽  
Maria H Styliou
Keyword(s):  
Lipid Metabolism ◽  
Cellulose Acetate ◽  
Rapid Method ◽  
Serum Lipoproteins ◽  
Simple Method

Abstract A simple, rapid method is described for separating the serum lipoproteins into clear, discrete, and reproducible bands by electrophoresis on cellulose acetate. Sera of patients with primary or secondary hyperlipidemias show patterns reflecting changes in lipid metabolism. The method would be appropriate for use in screening surveys.

scholarly journals Clonal variation within a mucosal isolate derived from a patient with Leishmania (Viannia) braziliensis infection

1991 ◽  
Vol 33 (5) ◽  
pp. 343-350 ◽  
Author(s):  
César Augusto Cuba-Cuba ◽  
David Evans ◽  
Ana de Cassia Rosa ◽  
Philip Davis Marsden
Keyword(s):  
Monoclonal Antibodies ◽  
Cellulose Acetate ◽  
Phenotypic Variation ◽  
Gel Electrophoresis ◽  
Clonal Variation ◽  
Starch Gel Electrophoresis ◽  
Mucosal Leishmaniasis ◽  
Starch Gel

Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and scrodemes analysis. Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated clear differences on Cellulose Acetate (CA) and thin starch gel electrophoresis. Also a panel of specific monoclonal antibodies showed such differences. Our observations provide additional evidence that Leishmania (Viannia) braziliensis is composed by subpopulations of parasites with peculiar biochemical and antigenic characteristics.

Electrophoresis of Plasma Lipoproteins on Gelatinized Cellulose Acetate

1971 ◽  
Vol 17 (6) ◽  
pp. 551-552 ◽  
Author(s):  
C Vergani ◽  
G L Rho ◽  
M Vannotti ◽  
G Vecchi
Keyword(s):  
Cellulose Acetate ◽  
Plasma Lipoproteins ◽  
Electrophoretic Separation ◽  
Serum Lipoproteins ◽  
Critical Step ◽  
Clear Cut ◽  
Large Sheet

Abstract A method for electrophoretic separation of serum lipoproteins on gelatinized cellulose acetate is described, which does not involve any critical step, and allows repeatable results with a clear-cut separation of beta, pre-beta, alpha-lipoproteins, and chylomicrons. Many samples can be examined simultaneously on a large sheet and the electropherograms are ready to be examined or densitometrically scanned in 2 h.

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