Low molecular weight carbohydrates and water-soluble polysaccharide metabolized by the cladophorales

1971 ◽  
Vol 10 (4) ◽  
pp. 807-812 ◽  
Author(s):  
Elizabeth Percival ◽  
Margaret Young
2013 ◽  
Vol 815 ◽  
pp. 293-298
Author(s):  
Li Jing Min ◽  
Jing Fen Li

LMW Branched Polyethylenimine-graft-lentinan was synthesized by performing crosslinker CDI in the presence of water-soluble Polysaccharide (Mw 70,000) and polyethylenimine (PEI, Mw 600). The chemistry of the PEI-g-lentinan obtained were characterized. The results indicated that the amines of lentinan were grafted with PEI. Gel electrophoresis showed that DNA migration was retarded completely at a N/P ratio of 10/1, indicating good DNA condensation capability of PEI-g-lentinan. The cytotoxicity of PEI-g-lentinan was evaluated, and the results reflected that PEI-g-lentinan had a lower cytotoxicity than PEI (25 K). Gene transfection efficiency of PEI-g-chitosan in cos-7 cells was determined. Remarkably,PEI-g-lentinan showed a higher transfection efficiency than that of PEI (25 K) in vitro.


2013 ◽  
Vol 13 (3) ◽  
Author(s):  
Netty Widyastuti ◽  
Teguh Baruji ◽  
Henky Isnawan ◽  
Priyo Wahyudi ◽  
Donowati Donowati

Beta glucan is a polysaccharide compound, generally not soluble inwater and resistant to acid. Beta glucan is used as an immunomodulator (enhancing the immune system) in mammals is usually a beta-glucan soluble in water, easily absorbed and has a low molecular weight. Several example of beta-glucan such as cellulose (β-1 ,4-glucan), lentinan (β-1 0.6-glucan) and (β-1 ,3-glucan), pleuran (β-1, 6 and β-1 ,3-glucan) are isolated from species of fungi Basidiomycota include mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes).The purpose of thisresearch activity is to obtain beta-glucan compound that can be dissolved in water and in alkali derived from fungi Basidiomycota, i.e, Oyster mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes). The result of beta-glucan compared to characterize the resulting beta glucan that is molecular structure . The difference of beta glucan extraction is based on the differences in solubility of beta-glucan. Beta glucan could be water soluble and insoluble water.


Cellulose ◽  
2011 ◽  
Vol 18 (4) ◽  
pp. 929-936 ◽  
Author(s):  
Yukiko Enomoto-Rogers ◽  
Hiroshi Kamitakahara ◽  
Arata Yoshinaga ◽  
Toshiyuki Takano

2013 ◽  
Vol 46 (6) ◽  
pp. 654-659 ◽  
Author(s):  
E. V. Shamrikova ◽  
I. V. Gruzdev ◽  
V. V. Punegov ◽  
F. M. Khabibullina ◽  
O. S. Kubik

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Hongtao Bi ◽  
Han Han ◽  
Zonghong Li ◽  
Weihua Ni ◽  
Yan Chen ◽  
...  

A water-soluble polysaccharide (BIWS-4b) was purified from the fruit bodies ofBulgaria inquinans(Fries). It is composed of mannose (27.2%), glucose (15.5%) and galactose (57.3%). Its molecular weight was estimated to be 7.4 kDa (polydispersity index, Mw/Mn: 1.35). Structural analyses indicated that BIWS-4b mainly contains (1→6)-linked, (1→5)-linked and (1→5,6)-linkedβ-Galfunits; (1→4)-linked and non-reducing terminalβ-Glcpunits; and (1→2)-linked, (1→6)-linked, (1→2,6)-linked and non-reducing terminalα-Manpunits. When examined by the 4-day method and in a prophylactic assay in mice, BIWS-4b exhibited markedly suppressive activity against malaria while enhancing the activity of artesunate. Immunological tests indicated that BIWS-4b significantly enhanced macrophage phagocytosis and splenic lymphocyte proliferation in malaria-bearing mice and normal mice. The anti-malarial activity of BIWS-4b might be intermediated by enhancing immune competence and restoring artesunate-suppressed immune function. Thus, BIWS-4b is a potential adjuvant of anti-malaria drugs.


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