The vibration characteristics of flexural vibration disk in ultrasonic vibration system are studied through experiments. The results show that the resonant frequency of flexural vibration disk reduces with the reducing of its thickness and the resonant frequency increases with the reducing of its diameter. When designing the ultrasonic vibration system, because the transformer horn and the flexural vibration disk are designedly integrated, the defects caused by the way of threaded connection can be avoided. It is found that flexural vibration is dominating in the disk with some reflected ultrasonic through experiments, at the same time, there exist amplifying characteristics in the vibration of the disk and the maximal amplitude can reach 18 μm. The corresponding results provide certain reference value for the structure design of ultrasonic vibration system and the application in ultrasonic vibration deep-hole honing.