Cultural Studies on Nitrate Reductase Deficient Nicotiana tabacum Mutant Protoplasts

1982 ◽  
Vol 105 (3) ◽  
pp. 219-227 ◽  
Author(s):  
D. Pental ◽  
S. Cooper-Bland ◽  
K. Harding ◽  
E.C. Cocking ◽  
A.J. Müller
Keyword(s):  
Nicotiana Tabacum ◽  
Cultural Studies ◽  
Nitrate Reductase

scholarly journals Characteristics of Nicotiana tabacum nitrate reductase protein produced in Saccharomyces cerevisiae

1991 ◽  
Vol 278 (2) ◽  
pp. 393-397 ◽  
Author(s):  
H N Truong ◽  
C Meyer ◽  
F Daniel-Vedele
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nicotiana Tabacum ◽  
Cytochrome C ◽  
Nitrate Reductase ◽  
Higher Plants ◽  
Reductase Activity ◽  
Molybdenum Cofactor ◽  
Biochemical Data ◽  
Native Form ◽  
Nr Activity

Tobacco nitrate reductase (NR) produced in yeast retains cytochrome c reductase activity, but not NR activity. Biochemical data suggest that the haem and FAD domains are functional, and that the molybdenum cofactor (MoCo) domain is inactive owing to the absence of MoCo in yeast. The native form of the produced NR is dimeric. Thus MoCo is not involved in NR dimerization in higher plants, contrary to current assumptions.

Somatic Hybridization of Nitrate Reductase Deficient Mutants of Nicotiana tabacum by Protoplast Fusion

1978 ◽  
Vol 44 (3) ◽  
pp. 273-277 ◽  
Author(s):  
KRISTINA GLIMELIUS ◽  
TAGE ERIKSSON ◽  
REINHARD GRAFE ◽  
ANDREAS J. MULLER
Keyword(s):  
Nicotiana Tabacum ◽  
Nitrate Reductase ◽  
Protoplast Fusion ◽  
Somatic Hybridization

Immunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplast fusion

1986 ◽  
Vol 204 (2) ◽  
pp. 296-301 ◽  
Author(s):  
D. A. Somers ◽  
K. R. Narayanan ◽  
A. Kleinhofs ◽  
S. Cooper-Bland ◽  
E. C. Cocking
Keyword(s):  
Nicotiana Tabacum ◽  
Nitrate Reductase ◽  
Protoplast Fusion ◽  
Structural Gene
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