Evidence for the presence of sex chromosomes in the Nile tilapia (Oreochromis niloticus) from synaptonemal complex analysis of XX, XY and YY genotypes

Some adaptations of the synaptonemal complex (SC) whole-mounting technique first used in plants permitted its application to meiotic studies in tilapia, Oreochromis niloticus. Direct observation of the chromosome pairing process and bivalent structure during the meiotic prophase of this fish species by light and electron microscopy permitted the analysis of SCs in autosomes and the possible identification of sex chromosomes. The analysis of SCs in spermatocytes of O. niloticus revealed that all 22 bivalent chromosomes completely paired, except for the occurrence of a size heteromorphism in the terminal region of the largest bivalent associated with the presence of an incompletely paired segment during the synapsis process, which may be the cytological visualization of an XX/XY sex chromosome system in this species.Key words: fish cytogenetics, synaptonemal complex, fish meiosis, sex chromosomes, nucleolus organizer regions.

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Synaptonemal Complex Analysis of Sex Chromosomes in Two Species of Sceloporus

Copeia ◽

10.2307/1446497 ◽

1990 ◽

Vol 1990 (4) ◽

pp. 1122 ◽

Cited By ~ 5

Author(s):

Kent M. Reed ◽

Philip D. Sudman ◽

Jack W. Sites ◽

Ira F. Greenbaum

Keyword(s):

Synaptonemal Complex ◽

Sex Chromosomes ◽

Complex Analysis ◽

Synaptonemal Complex Analysis

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Physical chromosome mapping of repetitive DNA sequences in Nile tilapia Oreochromis niloticus: Evidences for a differential distribution of repetitive elements in the sex chromosomes

Micron ◽

10.1016/j.micron.2007.02.010 ◽

2008 ◽

Vol 39 (4) ◽

pp. 411-418 ◽

Cited By ~ 51

Author(s):

Irani A. Ferreira ◽

Cesar Martins

Keyword(s):

Repetitive Dna ◽

Sex Chromosomes ◽

Dna Sequences ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Chromosome Mapping ◽

Repetitive Elements ◽

Repetitive Dna Sequences ◽

Differential Distribution ◽

Nile Tilapia Oreochromis Niloticus

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FISH and DAPI staining of the synaptonemal complex of the Nile tilapia (Oreochromis niloticus) allow orientation of the unpaired region of bivalent 1 observed during early pachytene

Chromosome Research ◽

10.1007/s10577-009-9071-9 ◽

2009 ◽

Vol 17 (6) ◽

pp. 773-782 ◽

Cited By ~ 15

Author(s):

Konrad Ocalewicz ◽

Jose C. Mota-Velasco ◽

Rafael Campos-Ramos ◽

David J. Penman

Keyword(s):

Synaptonemal Complex ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Dapi Staining ◽

Early Pachytene ◽

Nile Tilapia Oreochromis Niloticus ◽

Unpaired Region

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Characterization of Francisella noatunensis subsp. orientalis isolated from Nile tilapia Oreochromis niloticus farmed in Lake Yojoa, Honduras

Diseases of Aquatic Organisms ◽

10.3354/dao03333 ◽

2019 ◽

Vol 133 (2) ◽

pp. 141-145 ◽

Cited By ~ 2

Author(s):

E Soto ◽

K Shahin ◽

JJ Talhami ◽

MJ Griffin ◽

A Adams ◽

...

Keyword(s):

Oreochromis Niloticus ◽

Nile Tilapia ◽

Nile Tilapia Oreochromis Niloticus

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The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2014.7302 ◽

2014 ◽

pp. 27

Author(s):

Arne A Ratulangi ◽

Reiny Tumbol ◽

Hengky Manoppo ◽

Henneke Pangkey

Keyword(s):

Survival Rate ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Bacterial Disease ◽

Immersion Method ◽

Nile Tilapia Oreochromis Niloticus ◽

Different Ages ◽

Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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