The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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Infection prevention of Aeromonas hydrophila bacteria in Nile tilapia (Oreochromis niloticus) by providing ethyl acetate extract of temu ireng (Curcuma aeruginosa) rhizome

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f110201 ◽

2013 ◽

Vol 11 (2) ◽

pp. 31-35

Author(s):

DINA SELVIA SARI ◽

ARTINI PANGASTUTI ◽

ELISA HERAWATI

Keyword(s):

Ethyl Acetate ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Pathogenic Bacteria ◽

Infection Prevention ◽

Ethyl Acetate Extract ◽

Immersion Method ◽

Nile Tilapia Oreochromis Niloticus ◽

The One

Sari DS, Pangastuti A, Elisa Herawati E. 2013. Infection prevention of Aeromonas hydrophila bacteria in Nile tilapia (Oreochromis niloticus) by providing ethyl acetate extract of temu ireng (Curcuma aeruginosa) rhizome. Biofarmasi 11: 31-35. In tilapia aquaculture, some diseases can disturb the growth and production of fish. Aeromonas hydrophila is one of the pathogenic bacteria that can cause a disease in tilapia. Aeromonas hydrophila uses a quorum sensing system and the virulence of organisms as a controller to other organisms. The one of infection prevention effort of A. hydrophila that efficient enough is to use a compound of natural ingredients, i.e. Curcuma aeruginosa rhizome. The purpose of this study was to determine the optimal concentration of ethyl acetate extract of Curcuma aeruginosa rhizome that needed to prevent the infection of A. hydrophila bacterial in tilapia. The method used in this study was an immersion method. Tilapia was soaked in water mixed with A. hydrophila and the ethyl acetate extract of C. aeruginosa rhizome with the concentrations of 0 mL/L, 10 mL/L, 20 mL/L, 30 mL/L, 40 mL/L, 50 mL/L and control for 90 minutes. At the end of the study, it was observed for the fish behavior after immersion, the reaction of fish, the type and morphology of fish, and the number of bacteria in the water conservancy. The results showed that the A. hydrophila infection could be prevented by using the ethyl acetate extract of the C. aeruginosa rhizome with the concentration of 40 mL/L. During immersion, tilapia was get an experience stress, often to the surface of water, and then quietly at the bottom of aquarium. The response to eat of tilapia decreased by 50% after soaking, but after 2-3 days of immersion time, the fish feeding was normally again.

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