A comparative study on the virulence of entomopathogenic fungi against Trogoderma granarium (Everts) (Coleoptera: Dermestidae) in stored grains rice

Entomopathogenic fungi (EPF) now a possible safer microbial control measure that could be considered as a substitute for chemical control of insect pests. Three EPF viz., Metarihizium anisopliae, Isaria furnosoroseus and Beauveria bassiana were evaluated for their virulence against the grubs of Khapra beetle, Trogoderma granarium (Everts) under laboratory conditions. The isolates were applied by two methods viz., diet incorporation and an immersion method with 3rd instar 20 grubs of T. granarium for each. The virulence of EPF was determined using percent mortality. Significantly higher mortality was observed in M. anisopliae applied through immersion (98.33%) and diet incorporation (93.33%) methods followed by B. bassiana (90.83 and 85.83%, respectively). The mortality caused by I. furnosoroseus was statistically lower in immersion and diet incorporation methods i.e. 81.67 and 73.33%, respectively. Based on the immersion method, all EPF were studied for multiple conidial concentration i.e., 1×104, 1×105, 1×106, 1×107 and 1×108 under the same in-vitro conditions. All the isolates were pathogenic to grub of T. granarium at the highest conidial concentration. M. anisopliae was proved the most effective virulent resulting in 98.33% mortality of the pest with LT50 4.61 days at 1 × 108 conidial concentration followed by 90.83 and 81.67 percent mortality with 5.07 and 8.01 days LT50, in the application of B. bassiana and I. furnosoroseus, respectively. M. anisopliae showed higher efficacy and could be considered as promising EPF for the development of myco-insecticides against effective biocontrol of T. granarium.

Improving The Efficiency of Photovoltaic Cells by Using the Distilled Water Immersion Method

Photovoltaic panels can convert solar irradiance into (electrical and thermal) energy. The (PV / T) system was developed, created, and its performance tested in this experimental analysis. The main objective of this study was to design, manufacture and evaluate the work of the PV/T system as a thermal collector to enhance heat transfer, by using distilled water as a working fluid used to cool (PV/T) system. The experiment was performed with flow rate of water from (1 L / min to 5 L / min) on the PV / T collector channel. A theoretical and practical study was conducted on the effect of cooling the panels by immersing (PV) from (upper and lower) in a distilled water parallel flow forced circulation. Numerical result obtained by using Comsol Multiphysics program have been used as a computational fluid dynamic (CFD). The numerical study was conducted to determine the optimal depth of immersion of the panel to experiment with it, simulation results showed that the optimum depth of immersion is (5mm). The experimental results were conducted at the Technical Engineering College of Najaf with indoor test conditions that were controlled, Tin=20 °C, h=5mm. The results have been shown that the electrical efficiency of traditional photovolatic panel without cooling varied between (10.5-11.6) %, while the electrical efficiency of PV/T system varied between (14.6-14.7) %.

Potensi Ekstrak Daun Pepaya sebagai Inhibitor Korosi dalam Media Asam Klorida pada Baja ST37

Corrosion is a decrease in the quality of a metal material caused by a reaction with the environment. Reducing the corrosion rate on steel can be done by adding an inhibitor. Papaya leaf extract (Carica papaya.L) can be used as a corrosion inhibitor by immersion method. The effect of inhibitor on corrosion rate had been studied using weight loss method, potentiodynamic polarization method, and observation of steel surface using photooptic. It was found that corrosion rate decrease with increase concentration of papaya leaves extract. The highest inhibiton efficiency is 87.8% in 0.2 M hydrochloric acid with 2% concentration of papaya leaves extract. Potentiodynamic polarization method showed that papaya leaves extract decrease corrotion current. Inhibiton efficiency reached 81.58% in 2% extract concentration in which corrosion current decrease from 0.0266 mA/cm2 to 0.0049 mA/cm2. Analysis of photooptic showed that there is difference on steel surface corroded in hydrochloric acid with inhibitor and without it.

Antioxidant Activity Test of Effervescent Granules Morinda citrifolia L Leaf Extract with DPPH Free Radical Absorption Method

Morinda citrifolia. L leaf is a plant that has the potential as an antioxidant due to the presence of compounds in the form of flavonoids and other phenolic compounds that can function as natural antioxidants. In connection with this, research was carried out to Morinda citrifolia. L formulate leaf extract in the form of effervescent granules. Morinda citrifolia leaf extract was macerated with ethanol 96% as solvent. Phytochemical screening showsthat the extract contains flavonoids, tannins, saponins and alkaloids. The extract was formulated with various concentrations of F1 (20%), F2 (25%), F3 (4%). Determination of antioxidant activity using the DPPH free radical immersion method. The results showed that the concentration in the granules influenced antioxidant activity with IC50 values of 109.05 ppm, 101.33 ppm and 73.28 ppm, respectively

Cultural enrichment in Indonesian language for foreign speaker (BIPA) learning with Trihita Karana concept

Indonesian Language for foreign speaker (BIPA) students are very interested in cultural values, the provision of cultural values ​​is very important for BIPA students to enrich their language skills. Even cultural errors can be more serious than language errors. Trihita Karana is a philosophical value of living in harmony with God, nature, and fellow human beings. To teach BIPA students, it is necessary to have a genuine learning method so that BIPA students can absorb the culture significantly. The twenty BIPA learners in the State Polytechnic of Bali were conducted in this research. Questionnaire and observation methods were used in data collection. The data obtained were then analysed using a qualitative descriptive method. Based on the results of BIPA students' responses to several methods being tested, it can be concluded that 96.7% of respondents argued that the Trihita Karana cultural learning method was very feasible to support the BIPA learning process. There were several methods appreciated by BIPA students, including guessing pictures, running while dictation, observing, video documentation, immersion, and debate. Among these methods, the immersion method was the most favoured by BIPA students because they directly experienced it in daily life.

Reproductive toxicological changes in avian embryos due to a pesticide and an environmental contaminant

Single and simultaneous toxic effects of glyphosate (Amega Up, 360 g L−1, 4%) and copper sulphate (0.01%) were studied in avian embryos treated either with injection directly into the air chamber or by immersion application for 30 min on day 0 of incubation. Alterations of the chicken embryos were evaluated during necropsy performed on day 19 of incubation, together with mortality, body weight and the type of developmental abnormalities. Based on the results, the injection application appeared to be more toxic than the immersion method, as it induced increased mortality and reduced the average body weight, and resulted in a higher incidence of congenital anomalies. Supposedly, a toxicodynamic interaction occurs between copper sulphate and glyphosate, which may reduce the vitality of embryos and thus decrease the number of offspring in wild birds.

Material Properties of Strontium Doped Bioactive Glasses/Hydroxyapatite Composite and Its Mechanism of Promoting Bone Repair

Based on bioactive glasses (BG) of 58S, sol–gel method is used to prepare strontium oxide substituted bioactive glasses (SrO-BG) with different strontium content. SrO-BG and nano hydroxyapatite (HAp) composite materials were synthesized using precipitation method. The phase composition and morphologies of the prepared materials were examined by x-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques. The dissolution and bio-mineralization of SrO-BG and SrO-BG/HAp composites in SBF are investigated by immersion method. The effects of secretion components of macrophages regulated by strontium doped SrO-BG/HAp composites on the osteogenic differentiation (OD) of bone marrow mesenchymal stem cells (BMSCs) are analyzed. The results demonstrate that the SrO-BG can inhibit the dissolution of BG. Different proportions of SrO-BG/HAp composites show good ability to induce HAp in SBF. The bio-mineralization ability of SrO-BG/HAp composites increases with the increase of SrO-BG content. The results of dissolution behavior and bio-mineralization of SrO-BG/HAp composite show that the dissolution rate of each ion can be controlled by adjusting the content of SrO-BG in the composite, and then the degradation rate can effectively be controlled. The results of in vitro experiments show that SrO-BG/HAp composites with 2%, 5% and 8% strontium content are more effective in promoting M2 polarization of macrophages than SrO-BG/HAp composites with 0% strontium content. Among them, 5% strontium doped SrO-BG/HAp has the strongest effect on M2 polarization of macrophages, and the secretion of macrophages regulated by 5% strontium doped SrO-BG/HAp composite is more conducive to bone repair.

Sustainable Production of Roofing Tiles Using Glycerine Pitch as An Alternative Binder

A large quantity of glycerine pitch (GP) generated from the oleo-chemical industry has led to significant environmental issues when it is disposed of without proper treatment. This study investigated the possibility of utilizing glycerine pitch as an alternative binder in the production of roofing tile, namely GP-RT. The percentage of GP incorporated by weight of tile ranges from 3 – 9 %. A transverse strength test was performed on the tiles in dry and wet conditions to investigate their durability in harsh conditions. A water absorption test was performed to determine the water uptake of the tiles produced. The specimen with the greatest flexural strength was achieved when 7% of GP was utilized. The mechanical properties of the tile can be enhanced when GP was mixed with a similar amount of used cooking oil (UCO), which achieved the greatest strength (4389 N and 9.48 MPa) when 5% of GP and 5% of UcO were used for the tile’s production. The water resistivity of the tiles can be enhanced by the coating process. The lowest percentage of water absorption (2.82%) and the greatest wet transverse strength (2746 N) were achieved when the tile was coated with a layer of UCO via the immersion method. Conclusively, the full replacement of cement with GP as an alternative binder is possible. The incorporation of multiple waste materials (GP, UCO, and fly ash) in tile production is a feasible attempt to reduce the disposal of these waste materials to landfills, thus preserves a greener environment for future generations.

Evaluation of entomopathogenic fungi by larval and adult immersion method v1

The search for commercially viable entomopathogenic fungi for use in integrated pest management programs involves several steps. Fungal species must first be obtained from diseased insects or the environment and identified. Then, they must be evaluated under laboratory conditions to identify the most promising candidates. Because of that, bioassays must be repeatable and reliable to determine accurate pathogenicity or virulence. Variability in results may be caused by the variation in the components of an assay. However, the availability of a standardized bioassay is limited. Few reports detail the methods used to develop bioassays for a specific purpose and, without these details, it is difficult to develop bioassay methodologies suitable to evaluate the fungus-host relationship. We described a protocol based on the immersion method to evaluate entomopathogenic fungi (larval and adult stages), that can be reproduced to reduce variability. This protocol can be used in several stages of biopesticide development: selection of the biological control agent, characterization of the microorganism, formulation compatibility, and in vitro evaluation of efficacy.