[23] Calcium-induced permeabilization of the outer membrane: A method for reconstitution of periplasmic binding protein-dependent transport systems in Escherichia coli and Salmonella typhimurium

1986 ◽  
pp. 289-302 ◽  
Author(s):  
Johann M. Brass
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Binding Protein ◽  
Transport Systems ◽  
Periplasmic Binding Protein ◽  
Dependent Transport

Periplasmic binding protein-dependent transport systems: the membrane-associated components

1990 ◽  
Vol 326 (1236) ◽  
pp. 353-365 ◽  
Keyword(s):  
Transport System ◽  
Atp Hydrolysis ◽  
Binding Protein ◽  
Primary Source ◽  
Energy Coupling ◽  
Transport Systems ◽  
Common Mechanism ◽  
Atp Binding ◽  
Periplasmic Binding Protein ◽  
Dependent Transport

Periplasmic binding protein-dependent transport systems are multicomponent, consisting of several inner membrane-associated proteins and a periplasmic component. The membrane-associated components of different systems are related in organization and function suggesting that, despite different substrate specificities, each transport system functions by a common mechanism. Current understanding of these components is reviewed. The nature of energy coupling to periplasmic transport systems has long been debated. Recent data now demonstrate that ATP hydrolysis is the primary source of energy for transport. The ATP-binding transport components are the best characterized of a family of closely related ATP-binding proteins believed to couple ATP hydrolysis to a variety of different biological processes. Intriguingly, systems closely related to periplasmic binding protein-dependent transport systems have recently been identified in several Gram-positive organisms (which lack a periplasm) and in eukaryotic cells. This class of transport system appears to be widespread in nature, serving a variety of important and diverse functions.

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